CLARISSE MARTINS MACHADO
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Moléstias Infecciosas e Parasitárias, Faculdade de Medicina
LIM/52 - Laboratório de Virologia, Hospital das Clínicas, Faculdade de Medicina
LIM/52 - Laboratório de Virologia, Hospital das Clínicas, Faculdade de Medicina
3 resultados
Resultados de Busca
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- Poor response to hepatitis A vaccination in hematopoietic stem cell transplant recipients(2020) ADATI, Elen Monteiro; SILVA, Paula Moreira da; SUMITA, Laura Massami; RODRIGUES, Marina de Oliveira; ZANETTI, Lilian P.; SANTOS, Ana Claudia F. dos; SOUZA, Mair P. de; COLTURATO, Vergilio R.; MACHADO, Clarisse M.Background Hepatitis A virus (HAV) infection is highly prevalent in developing countries. In countries experiencing a shift from intermediate/high endemicity to low endemicity, the World Health Organization recommends the incorporation of HAV vaccine into the national vaccination calendar for children aged >= 1 year. Since HAV antibodies wane over time, most HSCT revaccination guidelines advise vaccination as optional, following the country recommendation. However, no study has evaluated the serological response to HAV vaccine in allogeneic HSCT recipients. Methods We conducted a prospective study in 46 HSCT recipients who received two doses of inactivated HAV vaccine. Blood samples were taken before vaccination to determine HAV prevalence rates, and before and 4-6 weeks after the second dose. Specific anti-HAV antibodies were detected by a competitive commercial enzyme immune assay. Results Patients received the first dose of vaccine at a median of 332.5 (120-4134) days after HSCT. Median absolute lymphocyte count at vaccination was 1947 (696-12 500)/mm(3). The seroprevalence rate was 93.5% at inclusion. Although safe and well tolerated, the serological response to HAV vaccine in susceptible patients was poor (33%), and no boost effect was observed in seropositive patients. Conclusions In areas with intermediate/high seroprevalence of HAV, serology should be recommended prior to referral to vaccination. The mechanisms of antibody interference and how to overcome T-cell function deficiency need to be better understood in transplant populations receiving HAV vaccine. Alternative schedules of HAV vaccination should be evaluated in prospective trials.
conferenceObject The Cumulative Incidence of Multiple DNA Virus Reactivation is not Different in Non-depleted Haploidentical Stem Cell Transplantation and Unrelated Allogeneic Stem Cell Transplantation(2020) KERBAUY, Mariana Nassif; RIBEIRO, Andreza Alice Feitosa; KERBAUY, Lucila Nassif; SILVA, Cinthya Correa da; HAMERSCHLAK, Nelson; MACHADO, Clarisse Martins- Influenza A and B in a cohort of outpatient children and adolescent with influenza like-illness during two consecutive influenza seasons(2020) MACHADO, Clarisse M.; SOUZA, Ana Carolina Mamana Fernandes de; ROMANO, Camila Malta; FREIRE, Wilton dos Santos; COSTA, Angela Aparecida; FIGUEIREDO, Walter Manso; PANNUTI, Claudio S.; LUNA, Expedito J. A.Introduction: Influenza is an important cause of morbimortality worldwide. Although people at the extremes of age have a greater risk of complications, influenza has been more frequently investigated in the elderly than in children, and inpatients than outpatients. Yearly vaccination with trivalent or quadrivalent vaccines is the main strategy to control influenza. Objectives: Determine the clinical and molecular characteristics of influenza A and B infections in children and adolescents with influenza-like illness (ILI). Methods: A cohort of outpatient children and adolescents with ILI was followed for 20 months. Influenza was diagnosed with commercial multiplex PCR platforms. Results: 179 patients had 277 episodes of ILI, being 79 episodes of influenza A and 20 episodes of influenza B. Influenza A and B cases were mild and had similar presentation. Phylogenetic tree of influenza B viruses showed that 91.6% belonged to the B/Yamagata lineage, which is not included in trivalent vaccines. Conclusions: Influenza A and B are often detected in children and adolescents with ILI episodes, with similar and mild presentation in outpatients. The mismatch between the circulating influenza viruses and the trivalent vaccine offered in Brazil may have contributed to the high frequency of influenza A and B in this population. (C) 2020 Sociedade Brasileira de Infectologia.