GILSON MASAHIRO MURATA
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Clínica Médica, Faculdade de Medicina
LIM/29 - Laboratório de Nefrologia Celular, Genética e Molecular, Hospital das Clínicas, Faculdade de Medicina
LIM/29 - Laboratório de Nefrologia Celular, Genética e Molecular, Hospital das Clínicas, Faculdade de Medicina
6 resultados
Resultados de Busca
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- Endoplasmic Reticulum Stress and Autophagy Markers in Soleus Muscle Disuse-Induced Atrophy of Rats Treated with Fish Oil(2021) MARZUCA-NASSR, Gabriel Nasri; KUWABARA, Wilson Mitsuo Tatagiba; VITZEL, Kaio Fernando; MURATA, Gilson Masahiro; TORRES, Rosangela Pavan; MANCINI-FILHO, Jorge; ALBA-LOUREIRO, Tatiana Carolina; CURI, RuiEndoplasmic reticulum stress (ERS) and autophagy pathways are implicated in disuse muscle atrophy. The effects of high eicosapentaenoic (EPA) or high docosahexaenoic (DHA) fish oils on soleus muscle ERS and autophagy markers were investigated in a rat hindlimb suspension (HS) atrophy model. Adult Wistar male rats received daily by gavage supplementation (0.3 mL per 100 g b.w.) of mineral oil or high EPA or high DHA fish oils (FOs) for two weeks. Afterward, the rats were subjected to HS and the respective treatments concomitantly for an additional two-week period. After four weeks, we evaluated ERS and autophagy markers in the soleus muscle. Results were analyzed using two-way analysis of variance (ANOVA) and Bonferroni post hoc test. Gastrocnemius muscle omega-6/omega-3 fatty acids (FAs) ratio was decreased by both FOs indicating the tissue incorporation of omega-3 fatty acids. HS altered (p < 0.05) the protein content (decreasing total p38 and BiP and increasing p-JNK2/total JNK2 ratio, and caspase 3) and gene expressions (decreasing BiP and increasing IRE1 and PERK) of ERS and autophagy (decreasing Beclin and increasing LC3 and ATG14) markers in soleus. Both FOs attenuated (p < 0.05) the increase in PERK and ATG14 expressions induced by HS. Thus, both FOs could potentially attenuate ERS and autophagy in skeletal muscles undergoing atrophy.
- Recreational Dance Practice Modulates Lymphocyte Profile and Function in Diabetic Women(2021) PASSOS, Maria Elizabeth Pereira; BORGES, Leandro; SANTOS-OLIVEIRA, Laiane Cristina dos; ALECRIM-ZEZA, Amanda Lins; LOBATO, Tiago Bertola; OLIVEIRA, Heloisa Helena de; SANTOS, Cesar Miguel Momesso; DINIZ, Vinicius Leonardo Sousa; ISER-BEM, Patricia Nancy; MANOEL, Richelieau; MURATA, Gilson Masahiro; HIRABARA, Sandro Massao; CURI, Rui; PITHON-CURI, Tania Cristina; HATANAKA, Elaine; GORJAO, RenataThis study aimed to investigate the impact of a 16-week dance-based aerobic exercise program on lymphocyte function in healthy and type 2 diabetes mellitus (T2DM) women. We enrolled 23 women: 11 with T2DM and 12 non-diabetic controls. Initially, we performed anthropometry and body composition measurements, afterwards, plasma levels of C-reactive protein, lipids, and glucose were determined. We used flow cytometry to measure the CD25 and CD28 expression in circulating lymphocytes, T-regulatory (Treg) cell percentage, lymphocyte proliferation, and cytokines released by cultured lymphocytes. The T2DM group had a lower proportion of CD28+ cells and a higher percentage of Treg lymphocytes and proliferative capacity at the baseline compared with the control group. After 16 weeks of the program, differences in lymphocytes between the T2DM and the control groups disappeared. The dance program promoted IL-10 increase in both groups. We found decreased IL-4, IL-2, and IL-6 secretion in lymphocytes from the control group and increased IL-17 secretion and IL-10/IL-17 ratio in the T2DM group after the program. The program promoted marked changes in lymphocytes in diabetic women, leading to a balance between the different profiles.
- Small intestine remodeling in male Goto-Kakizaki rats(2021) PEREIRA, Joice Naiara Bertaglia; MURATA, Gilson Masahiro; SATO, Fabio Takeo; MAROSTI, Aline Rosa; CARVALHO, Carla Roberta de Oliveira; CURI, RuiBackground: Obesity is associated with the development of insulin resistance (IR) and type-2 diabetes mellitus (T2DM); however, not all patients with T2DM are obese. The Goto-Kakizaki (GK) rat is an experimental model of spontaneous and non-obese T2DM. There is evidence that the intestine contributes to IR development in GK animals. This information prompted us to investigate small intestine remodeling in this animal model. Methods: Four-month-old male Wistar (control) and GK rats were utilized for the present study. After removing the small intestine, the duodenum, proximal jejunum, and distal ileum were separated. We then measured villi and muscular and mucosa layer histomorphometry, goblet cells abundance, total myenteric and submucosal neuron populations, and inflammatory marker expression in the small intestinal segments and intestinal transit of both groups of animals. Key Results: We found that the GK rats exhibited decreased intestinal area (p < 0.0001), decreased crypt depth in the duodenum (p = 0.01) and ileum (p < 0.0001), increased crypt depth in the jejunum (p < 0.0001), longer villi in the jejunum and ileum (p < 0.0001), thicker villi in the duodenum (p < 0.01) and ileum (p < 0.0001), thicker muscular layers in the duodenum, jejunum, and ileum (p < 0.0001), increased IL-1 beta concentrations in the duodenum and jejunum (p < 0.05), and increased concentrations of NF-kappa B p65 in the duodenum (p < 0.01), jejunum and ileum (p < 0.05). We observed high IL-1 beta reactivity in the muscle layer, myenteric neurons, and glial cells of the experimental group. GK rats also exhibited a significant reduction in submucosal neuron density in the jejunum and ileum, ganglionic hypertrophy in all intestinal segments studied (p < 0.0001), and a slower intestinal transit (about 25%) compared to controls. Conclusions: The development of IR and T2DM in GK rats is associated with small intestine remodeling that includes marked alterations in small intestine morphology, local inflammation, and reduced intestinal transit.
- A probiotic has differential effects on allergic airway inflammation in A/J and C57BL/6 mice and is correlated with the gut microbiome (vol 9, 134, 2021)(2021) CASARO, Mateus B.; THOMAS, Andrew M.; MENDES, Eduardo; FUKUMORI, Claudio; RIBEIRO, Willian R.; OLIVEIRA, Fernando A.; CRISMA, Amanda R.; MURATA, Gilson M.; BIZZARRO, Bruna; SA-NUNES, Anderson; SETUBAL, Joao C.; MAYER, Marcia P. A.; MARTINS, Flaviano S.; VIEIRA, Angelica T.; ANTIORIO, Ana T. F. B.; TAVARES-DE-LIMA, Wothan; CAMARA, Niels O. S.; CURI, Rui; DIAS-NETO, Emmanuel; FERREIRA, Caroline M.
- A probiotic has differential effects on allergic airway inflammation in A/J and C57BL/6 mice and is correlated with the gut microbiome(2021) CASARO, Mateus B.; THOMAS, Andrew M.; MENDES, Eduardo; FUKUMORI, Claudio; RIBEIRO, Willian R.; OLIVEIRA, Fernando A.; CRISMA, Amanda R.; MURATA, Gilson M.; BIZZARRO, Bruna; SA-NUNES, Anderson; SETUBAL, Joao C.; MAYER, Marcia P. A.; MARTINS, Flaviano S.; VIEIRA, Angelica T.; ANTIORIO, Ana T. F. B.; TAVARES-DE-LIMA, Wothan; CAMARA, Niels O. S.; CURI, Rui; DIAS-NETO, Emmanuel; FERREIRA, Caroline M.The phenotypes of allergic airway diseases are influenced by the interplay between host genetics and the gut microbiota, which may be modulated by probiotics. We investigated the probiotic effects on allergic inflammation in NJ and C57BU6 mice. C57BU6 mice had increased gut microbiota diversity compared to NJ mice at baseline. Acetate producer probiotics differentially modulated and altered the genus abundance of specific bacteria, such as Akkermansia and Allistipes, in mouse strains. We induced airway inflammation followed by probiotic treatment and found that only A/J mice exhibited decreased inflammation, and the beneficial effects of probiotics in NJ mice were partially due to acetate production. To understand the relevance of microbial composition colonization in the development of allergic diseases, we implanted female C57BU6 mice with NJ embryos to naturally modulate the microbial composition of NJ mice, which increased gut microbiota diversity and reduced eosinophilic inflammation in A/J. These data demonstrate the central importance of microbiota to allergic phenotype severity.
- Changes in Skeletal Muscle Protein Metabolism Signaling Induced by Glutamine Supplementation and Exercise(2023) JR, Carlos Flores Rodrigues; MURATA, Gilson Masahiro; GERLINGER-ROMERO, Frederico; NACHBAR, Renato Tadeu; MARZUCA-NASSR, Gabriel Nasri; GORJAO, Renata; VITZEL, Kaio Fernando; HIRABARA, Sandro Massao; PITHON-CURI, Tania Cristina; CURI, RuiAim: To evaluate the effects of resistance exercise training (RET) and/or glutamine supplementation (GS) on signaling protein synthesis in adult rat skeletal muscles. Methods: The following groups were studied: (1) control, no exercise (C); (2) exercise, hypertrophy resistance exercise training protocol (T); (3) no exercise, supplemented with glutamine (G); and (4) exercise and supplemented with glutamine (GT). The rats performed hypertrophic training, climbing a vertical ladder with a height of 1.1 m at an 80 degrees incline relative to the horizontal with extra weights tied to their tails. The RET was performed three days a week for five weeks. Each training session consisted of six ladder climbs. The extra weight load was progressively increased for each animal during each training session. The G groups received daily L-glutamine by gavage (one g per kilogram of body weight per day) for five weeks. The C group received the same volume of water during the same period. The rats were euthanized, and the extensor digitorum longus (EDL) muscles from both hind limbs were removed and immediately weighed. Glutamine and glutamate concentrations were measured, and histological, signaling protein contents, and mRNA expression analyses were performed. Results: Supplementation with free L-glutamine increased the glutamine concentration in the EDL muscle in the C group. The glutamate concentration was augmented in the EDL muscles from T rats. The EDL muscle mass did not change, but a significant rise was reported in the cross-sectional area (CSA) of the fibers in the three experimental groups. The levels of the phosphorylated proteins (pAkt/Akt, pp70S6K/p70S6K, p4E-BP1/4E-BP1, and pS6/S6 ratios) were significantly increased in EDL muscles of G rats, and the activation of p4E-BP1 was present in T rats. The fiber CSAs of the EDL muscles in T, G, and GT rats were increased compared to the C group. These changes were accompanied by a reduction in the 26 proteasome activity of EDL muscles from T rats. Conclusion: Five weeks of GS and/or RET induced muscle hypertrophy, as indicated by the increased CSAs of the EDL muscle fibers. The increase in CSA was mediated via the upregulated phosphorylation of Akt, 4E-BP1, p70S6k, and S6 in G animals and 4E-BP1 in T animals. In the EDL muscles from T animals, a decrease in proteasome activity, favoring a further increase in the CSA of the muscle fibers, was reported.