GILSON MASAHIRO MURATA
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Clínica Médica, Faculdade de Medicina
LIM/29 - Laboratório de Nefrologia Celular, Genética e Molecular, Hospital das Clínicas, Faculdade de Medicina
LIM/29 - Laboratório de Nefrologia Celular, Genética e Molecular, Hospital das Clínicas, Faculdade de Medicina
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- Effects of Resistance Exercise on Slow-Twitch Soleus Muscle of Infarcted Rats(2023) SOUZA, Lidiane Moreira; GOMES, Mariana Janini; BRANDAO, Bruna Brasil; PAGAN, Luana Urbano; GATTO, Mariana; DAMATTO, Felipe Cesar; RODRIGUES, Eder Anderson; PONTES, Thierres Hernani Dias; BORIM, Patricia Aparecida; FERNANDES, Ana Angelica Henrique; MURATA, Gilson Masahiro; ZORNOFF, Leonardo Antonio Mamede; AZEVEDO, Paula Schmidt; OKOSHI, Katashi; OKOSHI, Marina PolitiAlthough current guidelines recommend resistance exercise in combination with aerobic training to increase muscle strength and prevent skeletal muscle loss during cardiac remodeling, its effects are not clear. In this study, we evaluated the effects of resistance training on cardiac remodeling and the soleus muscle in long-term myocardial infarction (MI) rats. Methods: Three months after MI induction, male Wistar rats were assigned to Sham (n = 14), MI (n = 9), and resistance exercised MI (R-MI, n = 13) groups. The rats trained three times a week for 12 weeks on a climbing ladder. An echocardiogram was performed before and after training. Protein expression of the insulin-like growth factor (IGF)-1/protein kinase B (Akt)/rapamycin target complex (mTOR) pathway was analyzed by Western blot. Results: Mortality rate was higher in MI than Sham; in the R-MI group, mortality rate was between that in MI and Sham and did not differ significantly from either group. Exercise increased maximal load capacity without changing cardiac structure and left ventricular function in infarcted rats. Infarction size did not differ between infarcted groups. Catalase activity was lower in MI than Sham and glutathione peroxidase lower in MI than Sham and R-MI. Protein expression of p70S6K was lower in MI than Sham and p-FoxO3 was lower in MI than Sham and R-MI. Energy metabolism did not differ between groups, except for higher phosphofrutokinase activity in R-MI than MI. Conclusion: Resistance exercise is safe and increases muscle strength regardless structural and functional cardiac changes in myocardial-infarcted rats. This exercise modality attenuates soleus glycolytic metabolism changes and improves the expression of proteins required for protein turnover and antioxidant response.
- The Symbiotic Effect of a New Nutraceutical with Yeast beta-Glucan, Prebiotics, Minerals, and Silybum marianum (Silymarin) for Recovering Metabolic Homeostasis via Pgc-1 alpha, Il-6, and Il-10 Gene Expression in a Type-2 Diabetes Obesity Model(2022) SANTAMARINA, Aline Boveto; MORAES, Ruan Carlos Macedo; NEHMI FILHO, Victor; MURATA, Gilson Masahiro; FREITAS, Jessica Alves de; MIRANDA, Danielle Araujo de; CERQUEIRA, Anderson Romerio Azevedo; COSTA, Soraia Katia Pereira; FERREIRA, Ana Flavia Fernandes; BRITTO, Luiz Roberto; CAMARGO, Juliana Alves de; OLIVEIRA, Daniela Rodrigues de; JESUS, Flavia Neto de; OTOCH, Jose Pinhata; PESSOA, Ana Flavia MarcalThe use of natural products and derivatives for the prevention and control of non-communicable chronic diseases, such as type-2 diabetes (T2D), obesity, and hepatic steatosis is a way to achieve homeostasis through different metabolic pathways. Thus, male C57BL/6 mice were divided into the following groups: high-fat diet (HFD) vehicle, HFD + Supplemented, HFD + Supplemented_S, and isolated compounds. The vehicle and experimental formulations were administered orally by gavage once a day over the four weeks of the diet (28 consecutive days). We evaluated the energy homeostasis, cytokines, and mitochondrial gene expression in these groups of mice. After four weeks of supplementation, only the new nutraceutical group (HFD + Supplemented) experienced reduced fasting glycemia, insulin, HOMA index, HOMA-beta, dyslipidemia, ectopic fat deposition, and hepatic fibrosis levels. Additionally, the PPAR gamma coactivator 1 alpha (Pgc-1 alpha), interleukin-6 (Il-6), and interleukin-10 (Il-10) gene expression were augmented, while hepatic steatosis decreased and liver parenchyma was recovered. The glutathione-S-transferase activity status was found to be modulated by the supplement. We discovered that the new nutraceutical was able to improve insulin resistance and hepatic steatosis mainly by regulating IL 6, IL 10, and Pgc-1 alpha. gene expression.
- Influence of Isolated Resistance Exercise on Cardiac Remodeling, Myocardial Oxidative Stress, and Metabolism in Infarcted Rats(2023) RODRIGUES, Eder Anderson; LIMA, Aline Regina Ruiz; GOMES, Mariana Janini; SOUZA, Lidiane Moreira; PONTES, Thierres Hernani Dias; PAGAN, Luana Urbano; MURATA, Gilson Masahiro; DAMATTO, Felipe Cesar; DEPRA, Igor Carvalho; REGO, Amanda Bergamo Goncalves Castro; REYES, David Rafael Abreu; ZORNOFF, Leonardo Antonio Mamede; OKOSHI, Katashi; OKOSHI, Marina PolitiIntroduction: Exercise is an important therapeutic strategy for preventing and treating myocardial infarction (MI)-induced cardiac remodeling and heart failure. However, the myocardial effects of resistance exercise on infarcted hearts are not completely established. In this study, we investigated the effects of resistance exercise on structural, functional, and molecular cardiac alterations in infarcted rats. Methods: Three months after MI induction or simulated surgery, Wistar rats were assigned into three groups: Sham (n = 14); MI (n = 9); and exercised MI (MI-Ex, n = 13). Exercised rats performed, 3 times a week for 12 weeks, four climbs on a ladder with progressive loads. Cardiac structure and left ventricle (LV) function were analyzed by echocardiogram. Myocyte diameters were evaluated in hematoxylin- and eosin-stained histological sections as the smallest distance between borders drawn across the nucleus. Myocardial energy metabolism, lipid hydroperoxide, malondialdehyde, protein carbonylation, and antioxidant enzyme activities were evaluated by spectrophotometry. Gene expressions of NADPH oxidase subunits were evaluated by RT-PCR. Statistical analyses were performed using ANOVA and Tukey or Kruskal-Wallis and Dunn's test. Results: Mortality did not differ between the MI-Ex and MI groups. MI had dilated left atrium and LV, with LV systolic dysfunction. Exercise increased the maximum load-carrying capacity, with no changes in cardiac structure or LV function. Myocyte diameters were lower in MI than in Sham and MI-Ex. Lactate dehydrogenase and creatine kinase activity were lower in MI than in Sham. Citrate synthase and catalase activity were lower in MI and MI-Ex than in Sham. Lipid hydroperoxide concentration was lower in MI-Ex than in MI. Nox2 and p22phox gene expressions were higher in MI-Ex than in Sham. Gene expression of Nox4 was higher in MI and MI-Ex than in Sham, and p47phox was lower in MI than in Sham. Conclusion: Late resistance exercise was safe in infarcted rats. Resistance exercise improved maximum load-carrying capacity, reduced myocardial oxidative stress, and preserved myocardial metabolism, with no changes in cardiac structure or left ventricle function in infarcted rats.