GILSON MASAHIRO MURATA
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Clínica Médica, Faculdade de Medicina
LIM/29 - Laboratório de Nefrologia Celular, Genética e Molecular, Hospital das Clínicas, Faculdade de Medicina
LIM/29 - Laboratório de Nefrologia Celular, Genética e Molecular, Hospital das Clínicas, Faculdade de Medicina
4 resultados
Resultados de Busca
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- Recreational Dance Practice Modulates Lymphocyte Profile and Function in Diabetic Women(2021) PASSOS, Maria Elizabeth Pereira; BORGES, Leandro; SANTOS-OLIVEIRA, Laiane Cristina dos; ALECRIM-ZEZA, Amanda Lins; LOBATO, Tiago Bertola; OLIVEIRA, Heloisa Helena de; SANTOS, Cesar Miguel Momesso; DINIZ, Vinicius Leonardo Sousa; ISER-BEM, Patricia Nancy; MANOEL, Richelieau; MURATA, Gilson Masahiro; HIRABARA, Sandro Massao; CURI, Rui; PITHON-CURI, Tania Cristina; HATANAKA, Elaine; GORJAO, RenataThis study aimed to investigate the impact of a 16-week dance-based aerobic exercise program on lymphocyte function in healthy and type 2 diabetes mellitus (T2DM) women. We enrolled 23 women: 11 with T2DM and 12 non-diabetic controls. Initially, we performed anthropometry and body composition measurements, afterwards, plasma levels of C-reactive protein, lipids, and glucose were determined. We used flow cytometry to measure the CD25 and CD28 expression in circulating lymphocytes, T-regulatory (Treg) cell percentage, lymphocyte proliferation, and cytokines released by cultured lymphocytes. The T2DM group had a lower proportion of CD28+ cells and a higher percentage of Treg lymphocytes and proliferative capacity at the baseline compared with the control group. After 16 weeks of the program, differences in lymphocytes between the T2DM and the control groups disappeared. The dance program promoted IL-10 increase in both groups. We found decreased IL-4, IL-2, and IL-6 secretion in lymphocytes from the control group and increased IL-17 secretion and IL-10/IL-17 ratio in the T2DM group after the program. The program promoted marked changes in lymphocytes in diabetic women, leading to a balance between the different profiles.
- The Symbiotic Effect of a New Nutraceutical with Yeast beta-Glucan, Prebiotics, Minerals, and Silybum marianum (Silymarin) for Recovering Metabolic Homeostasis via Pgc-1 alpha, Il-6, and Il-10 Gene Expression in a Type-2 Diabetes Obesity Model(2022) SANTAMARINA, Aline Boveto; MORAES, Ruan Carlos Macedo; NEHMI FILHO, Victor; MURATA, Gilson Masahiro; FREITAS, Jessica Alves de; MIRANDA, Danielle Araujo de; CERQUEIRA, Anderson Romerio Azevedo; COSTA, Soraia Katia Pereira; FERREIRA, Ana Flavia Fernandes; BRITTO, Luiz Roberto; CAMARGO, Juliana Alves de; OLIVEIRA, Daniela Rodrigues de; JESUS, Flavia Neto de; OTOCH, Jose Pinhata; PESSOA, Ana Flavia MarcalThe use of natural products and derivatives for the prevention and control of non-communicable chronic diseases, such as type-2 diabetes (T2D), obesity, and hepatic steatosis is a way to achieve homeostasis through different metabolic pathways. Thus, male C57BL/6 mice were divided into the following groups: high-fat diet (HFD) vehicle, HFD + Supplemented, HFD + Supplemented_S, and isolated compounds. The vehicle and experimental formulations were administered orally by gavage once a day over the four weeks of the diet (28 consecutive days). We evaluated the energy homeostasis, cytokines, and mitochondrial gene expression in these groups of mice. After four weeks of supplementation, only the new nutraceutical group (HFD + Supplemented) experienced reduced fasting glycemia, insulin, HOMA index, HOMA-beta, dyslipidemia, ectopic fat deposition, and hepatic fibrosis levels. Additionally, the PPAR gamma coactivator 1 alpha (Pgc-1 alpha), interleukin-6 (Il-6), and interleukin-10 (Il-10) gene expression were augmented, while hepatic steatosis decreased and liver parenchyma was recovered. The glutathione-S-transferase activity status was found to be modulated by the supplement. We discovered that the new nutraceutical was able to improve insulin resistance and hepatic steatosis mainly by regulating IL 6, IL 10, and Pgc-1 alpha. gene expression.
- Intramuscular Injection of miR-1 Reduces Insulin Resistance in Obese Mice(2021) RODRIGUES, Alice C.; SPAGNOL, Alexandre R.; FRIAS, Flavia de Toledo; MENDONCA, Mariana de; ARAUJO, Hygor N.; GUIMARAES, Dimitrius; SILVA, William J.; BOLIN, Anaysa Paola; MURATA, Gilson Masahiro; SILVEIRA, LeonardoThe role of microRNAs in metabolic diseases has been recognized and modulation of them could be a promising strategy to treat obesity and obesity-related diseases. The major purpose of this study was to test the hypothesis that intramuscular miR-1 precursor replacement therapy could improve metabolic parameters of mice fed a high-fat diet. To this end, we first injected miR-1 precursor intramuscularly in high-fat diet-fed mice and evaluated glucose tolerance, insulin sensitivity, and adiposity. miR-1-treated mice did not lose weight but had improved insulin sensitivity measured by insulin tolerance test. Next, using an in vitro model of insulin resistance by treating C2C12 cells with palmitic acid (PA), we overexpressed miR-1 and measured p-Akt content and the transcription levels of a protein related to fatty acid oxidation. We found that miR-1 could not restore insulin sensitivity in C2C12 cells, as indicated by p-Akt levels and that miR-1 increased expression of Pgc1a and Cpt1b in PA-treated cells, suggesting a possible role of miR-1 in mitochondrial respiration. Finally, we analyzed mitochondrial oxygen consumption in primary skeletal muscle cells treated with PA and transfected with or without miR-1 mimic. PA-treated cells showed reduced basal respiration, oxygen consumption rate-linked ATP production, maximal and spare capacity, and miR-1 overexpression could prevent impairments in mitochondrial respiration. Our data suggest a role of miR-1 in systemic insulin sensitivity and a new function of miR-1 in regulating mitochondrial respiration in skeletal muscle.
- Novel Nutraceutical (silymarin, yeast & beta;-glucan, prebiotics, and minerals) shifts gut microbiota and restores large intestine histology of diet-induced metabolic syndrome mice(2023) NEHMI-FILHO, Victor; FREITAS, Jessica Alves de; FRANCO, Lucas Augusto Moyses; FONSECA, Joyce Vanessa da Silva; MARTINS, Roberta Cristina Ruedas; SANTAMARINA, Aline Boveto; MURATA, Gilson Masahiro; SABINO, Ester Cerdeira; SOUZA, Erica; FERREIRA, Matthew Thomas; OTOCH, Jose Pinhata; PESSOA, Ana Flavia MarcalGut dysbiosis contributes to chronic inflammation and oxidative stress associated with metabolic syndrome, and non-pharmacological, health-promoting supplements like nutraceuticals have been studied and used as a way to prevent or treat several illnesses. Thus, male C57BL/6 mice were divided into the following groups: control (CTL) _Vehicle and CTL_Nutraceutical; high-fat diet (HFD)_Vehicle, HFD_Nutraceutical, HFD_Nutraceutical_S, and isolated compounds. The vehicle and experimental formulations were administered by gavage once a day for four weeks with a high-fat diet simultaneously. In addition, we evaluated the composition of the fecal microbiota by partial 16S rRNA sequences directly amplified using a bacterial/archaeal primer set 515F/806R, and large intestine histomorphology was examined by H & E (Hematoxylin and Eosin), Masson's trichrome (MT), and PAS-AB (Periodic Acid Schiff-Alcian Blue) stains in these groups of mice. After four weeks of supplementation, only the Novel Nutraceutical supplement was able to increase & alpha; and & beta;-diversities, modulate the relative abundance in gut microbiota, decrease Firmicutes, and increase Bacteroidetes bacteria, meanwhile recovering the large intestine's (colon) histomorphology in CTL and HFD groups.