RICARDO DOS SANTOS SIMOES
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
SCGINEC-62, Hospital Universitário
LIM/58 - Laboratório de Ginecologia Estrutural e Molecular, Hospital das Clínicas, Faculdade de Medicina
SCGINEC-62, Hospital Universitário
LIM/58 - Laboratório de Ginecologia Estrutural e Molecular, Hospital das Clínicas, Faculdade de Medicina
171 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 171
conferenceObject The physical activity in postmenopause women and performance at Virtual Reality(2018) RAIMUNDO, Juliana Z.; RAIMUNDO, Rodrigo D.; ABREU, Luiz C. de; SOARES JUNIOR, Jose M.; SIMOES, Ricardo D.; NORBERTO, Alex R.; PEREIRA, Valdelias X.; SORPRESO, Isabel C.; BARACAT, Edmund C.bookPart Análogos do hormônio liberador de gonadotrofina(2014) JúNIOR, José Maria Soares; AMARAL, Vínicius Cestari do; BARACAT, Maria Cândida Pinheiro; SIMõES, Ricardo dos Santos; BARACAT, Edmund Chada- Isoflavones in gynecology(2018) CARBONEL, Adriana Aparecida Ferraz; SIMOES, Ricardo Santos; GIRAO, Joao Henrique Castello; SASSO, Gisela Rodrigues da Silva; BERTONCINI, Clelia Rejane Antonio; SORPRESO, Isabel Cristina Esposito; SOARES JUNIOR, Jose Maria; SIMOES, Manuel Jesus; BARACAT, Edmund Chada
- Glycosaminoglycan profiles in the uterus of adult ovariectomized rats treated with estrogen and progestagen(2012) SIMOES, Ricardo Santos; OLIVEIRA-FILHO, Ricardo Martins; NADER, Helena Bonciani; BARACAT, Edmund ChadaObjectives: To evaluate the effects of conjugated equine estrogens (CE) alone or in combination with medroxyprogesterone acetate (MPA) on glycosaminoglycans (GAGs) in the cervix and horns of the rat uterus. Study design: Thirty days after ovariectomy, adult rats were randomly divided into four groups: Cl, control (treated with drug vehicle); GII CE (50 mu g/kg per day); GIII, MPA (0.2 mg/kg per day), and GIV, CE + MPA (doses as in GII and Gill). Drugs and vehicle were given by gavage during 28 days. Afterwards the animals were anesthetized, the cervix and uterine horns were dissected out and the middle portion fixed in 10% formaldehyde solution; other portions were fixed in acetone for histological examination and glycosaminoglycan quantification, respectively. Agarose gel electrophoresis was used for sulfated GAG analyses, and hyaluronic acid was assayed with an ELISA-like method. Statistical analysis was done by the Student's t test and the Tukey-Kramer test (P < 0.05). Results: The cervix and uterine horn structures presented signs of atrophy in the control group (GI). The other groups, mainly groups III and IV, had histological aspects of proliferation. In all groups the concentration of sulfated GAGs (especially dermatan sulfate) was higher than that of non-sulfated GAGs, both in cervix and in uterine horns. Estrogens increased sulfated GAG concentration at the cervix and the horn, whereas in uterine horns the amounts of sulfated GAGs were decreased after estrogens plus MPA treatment. The concentration of hyaluronic acid in uterine horns was higher than in cervices. Conclusions: The profiling and amounts of glycosaminoglycans in the two portions of the rat uterus are uneven. Dermatan sulfate occurs in higher concentrations in both cervix and uterine horns. Sulfated GAGs in rat cervix were increased by estrogens plus MPA, but were decreased by MPA alone in uterine horns.
- Combined effects of ovariectomy and streptozotocin-induced diabetes in the articular cartilage of rats(2018) FERNANDES, A. Saeki; FONSECA, C. C. N.; SASSO, G. Rodrigues da Silva; CEZAR, L. Carvalho; SANTOS, M. Aparecida dos; SIMOES, M. J.; SIMOES, R. S.; FLORENCIO-SILVA, R.Aim: To evaluate the combined effects of streptozotocin-induced diabetes (Di) and ovariectomy in the articular cartilage of rats. Methods: Forty adult female Wistar rats were ovariectomized (OVX) or sham-operated. After recovery from surgery, the animals were assigned randomly into four groups: OVX control (OVX-C); OVX treated with 10 mg/kg/day of 17 beta-estradiol (OVX-E); sham-operated subjected to Di (Sham-Di); and OVX subjected to Di (OVX-Di). After 60 days of treatment, the animals were euthanized and the distal femurs with articular cartilage were processed for paraffin-embedding. Sections were stained with hematoxylin and eosin for histomorphometry, Picro-Sirius Red for collagen, or Alcian Blue for glycosaminoglycan (GAG) content. To detect apoptosis, sections were stained with an antibody to cleaved caspase-3 (casp-3). Results: Articular cartilage thickness and GAG content were significantly lower (p<0.05) in the OVX-Di group, which also showed a higher number of casp-3-positive chondrocytes than the other groups. Interestingly, the higher percentage (p<0.05) of mature collagen fibers was seen in the OVX-Di group, may be as a result of a reduced extracellular matrix remodeling of the articular cartilage. Conclusion: Our results indicate that the combination of ovariectomy and streptozotocin-induced diabetes produces more deleterious effects in articular cartilage of rats than either condition alone.
- Hyperprolactinemia modifies extracellular matrix components associated with collagen fibrillogenesis in harderian glands of non- and pregnant female mice(2023) ARAUJO, Ariadne S. L.; SIMOES, Manuel de J.; JR, Osvaldo P. Araujo; SIMOES, Ricardo S.; BARACAT, Edmund C.; NADER, Helena B.; JR, Jose M. Soares; GOMES, Regina C. T.The harderian gland (HG) is a gland located at the base of the nictating membrane and fills the inferomedial aspect of the orbit in rodents. It is under the influence of the hypothalamic-pituitary-gonadal axis and, because of its hormone receptors, it is a target tissue for prolactin (PRL) and sex steroid hormones (estrogen and progesterone). In humans and murine, the anterior surface of the eyes is protected by a tear film synthesized by glands associated with the eye. In order to understand the endocrine changes caused by hyperprolactinemia in the glands responsible for the formation of the tear film, we used an animal model with metoclopramide-induced hyperprolactinemia (HPRL). Given the evidences that HPRL can lead to a process of cell death and tissue fibrosis, the protein expression of small leucine-rich proteoglycans (SLRPs) was analyzed through immunohistochemistry in the HG of the non-and the pregnant female mice with hyperprolactinemia. The SRLPs are related to collagen fibrillogenesis and they participate in pro-apoptotic signals. Our data revealed that high prolactin levels and changes in steroid hormones (estrogen and progesterone) can lead to an alteration in the amount of collagen, and in the structure of type I and III collagen fibers through changes in the amounts of lumican and decorin, which are responsible for collagen fibrillogenesis. This fact can lead to the impaired functioning of the HG by excessive apoptosis in the HG of the non-and the pregnant female mice with HPRL and especially in the HG of pregnancy-associated hyperprolactinemia.
conferenceObject Expression of vascular endothelial growth factor (VEGF) in the endometrium of women with polycystic ovary syndrome (PCOS)(2013) SIMOES, Ricardo S.; SASSO, Gisela R.; FLORENCIO-SILVA, Rinaldo; MAGANHIN, Carla C.; SOARES- JR., Jose M.; BARACAT, Maria Candida P.; BARACAT, Edmund C.conferenceObject Melatonin may influence CYP19 immunoexpression in ovary of female rats in proestrous phase(2013) SASSO, Gisela R.; MAGANHIN, Carla C.; FUCHS, Luizf.; FRANCO, Paulo Celso; FLORENCIO-SILVA, Rinaldo; SIMOES, Ricardo S.; BARACAT, Edmund C.; SOARES- JR., Jose M.- Evidence that Melatonin Increases Inhibin Beta-A and Follistatin Gene Expression in Ovaries of Pinealectomized Rats(2020) MAGANHIN, Carla C.; BARACAT, Maria Candida P.; CARVALHO, Katia C.; SEGANFREDO, Isadora Braga; LUQUETTI, Camilla Maganhin; SIMOES, Ricardo Dos Santos; CARBONEL, Adriana Aparecida Ferraz; SIMOES, Manuel de Jesus; CIPOLLA-NETO, Jose; GIRAO, Manoel Joao Batista Castello; BARACAT, Edmund C.; SOARES-JR, Jose M.Melatonin plays an important role in the regulation of ovarian function including oocyte maturation in different mammalian species. Many studies indicate that melatonin has an impact on the ovarian function of a variety of ovarian cells. However, the information on the exact mechanism and involved hormones is low. To evaluate inhibin beta-A (INHBA) and follistatin (FST) expression in the ovaries of pinealectomized rats treated with melatonin, thirty adult female Wistar rats were randomized into three groups of ten animals each: group 1 (GSh), sham-operated controls receiving vehicle; group 2 (GPx), pinealectomized animals receiving vehicle; and group 3 (GPxMe), pinealectomized animals receiving replacement melatonin (1.0 mg/kg body weight. It was assumed that each animal drank 6.5 +/- 1.2 ml per night and weighs approximately 300 g.) for 60 consecutive days. The ovaries were collected for mRNA abundance and protein of INHBA and FST by qRT-PCR and immunohistochemical analyses, respectively. Treatment with melatonin resulted in the upregulation of INHBA and FST genes in the ovarian tissue of the melatonin-treated animals (GPxMe), when compared with GPx. These findings were then confirmed by analyzing the expression of protein by immunohistochemical analyses, which revealed higher immunoreactivity of INHBA and FST in GPxMe animals in the follicular cells compared with GSh and GPx rats. Melatonin increases the expression of INHBA and FST in the ovaries of pinealectomized female rats.
- Hormone therapy effect on menopausal systemic lupus erythematosus patients: a systematic review(2022) SOARES-JR, J. M.; SORPRESO, I. C. Esposito; CURADO, J. F. Nunes; FERREIRA FILHO, E. S.; SIMOES, R. dos Santos; BONFA, E.; SILVA, C. A.; BARACAT, E. C.Systemic lupus erythematosus (SLE) primarily affects women, who may need hormone therapy (HT) in menopause. There is, however, some concern as to its efficacy and safety. This systematic review aimed to determine the effect of HT on the activity of SLE and its safety. The study was a qualitative systematic review. Research was conducted with data retrieved from Embase, MEDLINE and Cochrane databases using MESH terms up to April 2021, with no bar on date or language. Sixteen studies were selected for analysis. Most of them showed HT to be effective in the treatment of menopausal symptoms with no impact in SLE activity, but one randomized clinical trial showed an increase in the number of thrombotic events. The present systematic review demonstrated the efficacy of HT for treating the menopausal symptoms of SLE patients. The risk of flare and thrombosis seems to be very low.