ANDERSON VICENTE DE PAULA

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Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina
LIM/52 - Laboratório de Virologia, Hospital das Clínicas, Faculdade de Medicina

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  • article 0 Citação(ões) na Scopus
    An international, interlaboratory ring trial confirms the feasibility of an extraction-less ""direct"" RT-qPCR method for reliable detection of SARS-CoV-2 RNA in clinical samples
    (2022) MILLS, Margaret G.; BRUCE, Emily; HUANG, Meei-Li; CROTHERS, Jessica W.; HYRIEN, Ollivier; OURA, Christopher A. L.; BLAKE, Lemar; JORDAN, Arianne Brown; HESTER, Susan; WEHMAS, Leah; MARI, Bernard; BARBY, Pascal; LACOUX, Caroline; FASSY, Julien; VIAL, Pablo; VIAL, Cecilia; MARTINEZ, Jose R. W.; OLADIPO, Olusola Olalekan; INUWA, Bitrus; SHITTU, Ismaila; MESEKO, Clement A.; CHAMMAS, Roger; SANTOS, Carlos Ferreira; DIONISIO, Thiago Jose; GARBIERI, Thais Francini; PARISI, Viviane Aparecida; MENDES-CORREA, Maria Cassia; PAULA, Anderson V. de; ROMANO, Camila M.; GOES, Luiz Gustavo Bentim; MINOPRIO, Paola; CAMPOS, Angelica C.; CUNHA, Marielton P.; VILELA, Ana Paula P.; NYIRENDA, Tonney; MKAKOSYA, Rajhab Sawasawa; MUULA, Adamson S.; DUMM, Rebekah E.; HARRIS, Rebecca M.; MITCHELL, Constance A.; PETTIT, Syril; BOTTEN, Jason; JEROME, Keith R.
    Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is used worldwide to test and trace the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). ""Extraction-less"" or ""direct"" real time-reverse transcription polymerase chain reaction (RT-PCR) is a transparent and accessible qualitative method for SARS-CoV-2 detection from nasopharyngeal or oral pharyngeal samples with the potential to generate actionable data more quickly, at a lower cost, and with fewer experimental resources than full RT-qPCR. This study engaged 10 global testing sites, including laboratories currently experiencing testing limitations due to reagent or equipment shortages, in an international interlaboratory ring trial. Participating laboratories were provided a common protocol, common reagents, aliquots of identical pooled clinical samples, and purified nucleic acids and used their existing in-house equipment. We observed 100% concordance across laboratories in the correct identification of all positive and negative samples, with highly similar cycle threshold values. The test also performed well when applied to locally collected patient nasopharyngeal samples, provided the viral transport media did not contain charcoal or guanidine, both of which appeared to potently inhibit the RT-PCR reaction. Our results suggest that direct RT-PCR assay methods can be clearly translated across sites utilizing readily available equipment and expertise and are thus a feasible option for more efficient COVID-19 coronavirus disease testing as demanded by the continuing pandemic.
  • article 32 Citação(ões) na Scopus
    Performance of at-home self-collected saliva and nasal-oropharyngeal swabs in the surveillance of COVID-19
    (2021) BRAZ-SILVA, Paulo H.; MAMANA, Ana C.; ROMANO, Camila M.; FELIX, Alvina C.; V, Anderson de Paula; FEREIRA, Noeli E.; BUSS, Lewis F.; TOZETTO-MENDOZA, Tania R.; V, Rafael A. Caixeta; LEAL, Fabio E.; GRESPAN, Regina M. Z.; BIZARIO, Joao C. S.; FERRAZ, Andrea B. C.; SAPKOTA, Dipak; GIANNECCHINI, Simone; TO, Kelvin K.; DOGLIO, Alain; MENDES-CORREA, Maria C.
    Background: SARS-CoV-2 quickly spreads in the worldwide population, imposing social restrictions to control the infection, being the massive testing another essential strategy to break the chain of transmission. Aim: To compare the performance of at-home self-collected samples - saliva and combined nasal-oropharyngeal swabs (NOP) - for SARS-CoV-2 detection in a telemedicine platform for COVID-19 surveillance. Material and methods: We analyzed 201 patients who met the criteria of suspected COVID-19. NOP sampling was combined (nostrils and oropharynx) and saliva collected using a cotton pad device. Detection of SARS-COV-2 was performed by using the Altona RealStar (R) SARS-CoV-2 RT-PCR Kit 1.0. Results: There was an overall significant agreement (kappa coefficient value of 0.58) between saliva and NOP. Considering results in either sample, 70 patients positive for SARS-CoV-2 were identified, with 52/70 being positive in NOP and 55/70 in saliva. This corresponds to sensitivities of 74.2% (95% CI; 63.7% to 83.1%) for NOP and 78.6% (95% CI; 67.6% to 86.6%) for saliva. Conclusion: Our data show the feasibility of using at-home self-collected samples (especially saliva), as an adequate alternative for SARS-CoV-2 detection. This new approach of testing can be useful to develop strategies for COVID-19 surveillance and for guiding public health decisions.
  • article 12 Citação(ões) na Scopus
    Zika virus infection among symptomatic patients from two healthcare centers in Sao Paulo State, Brazil: prevalence, clinical characteristics, viral detection in body fluids and serodynamics
    (2019) TOZETTO-MENDOZA, Tania Regina; AVELINO-SILVA, Vivian Iida; FONSECA, Silvia; CLARO, Ingra Morales; PAULA, Anderson Vicente de; LEVIN, Anna Sara; SABINO, Ester Cerdeira; MENDES-CORREA, Maria Cassia; FIGUEIREDO, Walter Manso; FELIX, Alvina Clara; SOUZA, Nathalia C. Santiago; COSTA, Angela Aparecida; INENAMI, Maria; SILVA, Rosangela M. Gasparetto da; LEVI, Jose Eduardo; ROMANO, Camila Malta; PARANHOS-BACCALA, Glaucia; SEGURADO, Aluisio Cotrim; MAYAUD, Philippe
    Zika virus (ZIKV) clinical presentation and frequency/duration of shedding need further clarification. Symptomatic ZIKV-infected individuals identified in two hospitals in Sao Paulo State, Brazil, were investigated regarding clinical characteristics, shedding in body fluids, and serodynamics. Ninety-four of 235 symptomatic patients (Site A: 58%; Site B: 16%) had Real-Time PCR-confirmed ZIKV infection; fever, headache and gastrointestinal symptoms were less frequent, and rash was more frequent compared to ZIKV-negative patients. Real-Time PCR in serum had worse performance compared to plasma, while urine had the highest sensitivity. Shedding in genital fluids and saliva was rare. IgM positivity was the highest <14 days after the symptoms onset (86%), decreasing >28 days (24%); IgG positivity increased >14 days (96%) remaining positive in 94% of patients >28 days. ZIKV prevalence varied importantly in two neighboring cities during the same transmission season. Urine Real-Time PCR can improve diagnostic sensitivity; serum testing is less useful. Accurate serological tests are needed to improve diagnosis and surveillance.