JOSE PINHATA OTOCH
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Cirurgia, Faculdade de Medicina - Docente
DVCLCIR-62, Hospital Universitário
LIM/26 - Laboratório de Pesquisa em Cirurgia Experimental, Hospital das Clínicas, Faculdade de Medicina - Líder
DVCLCIR-62, Hospital Universitário
LIM/26 - Laboratório de Pesquisa em Cirurgia Experimental, Hospital das Clínicas, Faculdade de Medicina - Líder
114 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 114
- Pain inhibition through transplantation of fetal neuronal progenitors into the injured spinal cord in rats(2019) BATISTA, Chary M.; MARIANO, Eric D.; DALE, Camila S.; CRISTANTE, Alexandre F.; BRITTO, Luiz R.; OTOCH, Jose P.; TEIXEIRA, Manoel J.; MORGALLA, Matthias; LEPSKI, GuilhermeNeuropathic pain after spinal cord injury (SCI) is a complex condition that responds poorly to usual treatments. Cell transplantation represents a promising therapy; nevertheless, the ideal cell type in terms of neurogenic potential and effectiveness against pain remains largely controversial. Here, we evaluated the ability of fetal neural stem cells (fNSC) to relieve chronic pain and, secondarily, their effects on motor recovery. Adult Wistar rats with traumatic SCI were treated, 10 days after injury, with intra-spinal injections of culture medium (sham) or fNSCs extracted from telencephalic vesicles (TV group) or the ventral medulla (VM group) of E/14 embryos. Sensory (von Frey filaments and hot plate) and motor (the Basso, Beattie, Bresnahan locomotor rating scale and inclined plane test) assessments were performed during 8 weeks. Thereafter, spinal cords were processed for immunofluorescence and transplanted cells were quantified by stereology. The results showed improvement of thermal hyperalgesia in the TV and VM groups at 4 and 5 weeks after transplantation, respectively. Moreover, mechanical allodynia improved in both the TV and VM groups at 8 weeks. No significant motor recovery was observed in the TV or VM groups compared with sham. Stereological analyses showed that similar to 70% of TV and VM cells differentiated into NeuN(+) neurons, with a high proportion of enkephalinergic and GABAergic cells in the TV group and enkephalinergic and serotoninergic cells in the VM group. Our study suggests that neuronal precursors from TV and VM, once implanted into the injured spinal cord, maturate into different neuronal subtypes, mainly GABAergic, serotoninergic, and enkephalinergic, and all subtypes alleviate pain, despite no significant motor recovery. The study was approved by the Animal Ethics Committee of the Medical School of the University of Sao Paulo (protocol number 033/14) on March 4, 2016.
- Robotic endoscopy. A review of the literature(2020) VISCONTI, Thiago Arantes de Carvalho; OTOCH, Jose Pinhata; ARTIFON, Everson Luiz de AlmeidaPurpose: To present new endoscopic robotic devices in the context of minimally invasive procedures with high precision and automation. Methods: Review of the literature by December 2018 on robotic endoscopy. Results: We present the studies and investments for robotic implementation and flexible endoscopy evolution. We divided them into forceps manipulation platforms, active endoscopy and endoscopic capsule. They try to improve forceps handling and stability and to promote active movement. Conclusion: The implementation and propagation of robotic models depend on doing what the endoscopist is unable to. The new devices are moving forward in this direction.
- Potential of colonoscopy as a treatment for intussusception in children(2017) TAFNER, Edmar; TAFNER, Philipe; MITTLEDORF, Cornelius; PINHATA, Jose; SILVA, Ana Luisa; PILLI, Simone; SILVA, Jose Guilherme da; HASSEGAWA, Renato T.; MARUTA, Luis; CHRISTIANO, Celso; ANDRADA, LincolnBackground and study aims Invagination, which can occur in any part of the intestine, usually affects children and is the second most common cause of intestinal obstruction after pyloric stenosis. The cause of these intussusceptions in children is usually unknown and they typically occur within the ileocolic region. Management of pediatric patients with the condition often consists of surgical intervention. However, this retrospective study from the University Hospital of the University of Sao Paulo, Brazil, reports a series of cases of intussusception in children in whom a colonoscopy was used to reverse the intussusception. Patients and methods From April 2010 to January 2015, 30 pediatric patients underwent a colonoscopy as an noninvasive method for treatment of children's intestinal intussusception. Results Overall, treatment with colonoscopy was successful in reversing invagination in 66.7% of the patients. However, 33.3% of patients required surgery to resolve the intussusception. Conclusion Noninvasive colonoscopy is a potential treatment for intussusception in children.
bookPart Endoscopia de Alta Tecnologia de Imagem(2015) CASTAñO, Rodrigo; CABARCAS, Edilberto Nuñez; FAVARO, Gabriel; OTOCH, José Pinhata; ARTIFON, Everson L. A.- Virtual reality simulator versus box-trainer to teach minimally invasive procedures: A meta-analysis(2019) GUEDES, Hugo Goncalo; FERREIRA, Zemia Maria Camara Costa; LEAO, Layra Ribeiro de Sousa; MONTERO, Edna Frasson Souza; OTOCH, Jose Pinhata; ARTIFON, Everson Luiz de AlmeidaBackground: To evaluate the effectiveness of virtual reality simulator (VRS) training compared to box-trainer training (BT) for learning outcomes in minimally invasive surgery (MIS) techniques. Materials and Methods: A systematic review of the literature was performed using CENTRAL, MEDLINE, EMBASE, Scopus, CINAHL, LILACS. The primary outcomes were time to perform MIS and performance score in MIS. After being selected, the articles were evaluated for methodological quality and risk of bias. The results were evaluated for quality of evidence and meta-analysis was performed. Results: 20 randomized clinical trials were included in the qualitative analysis and 14 were used in the meta-analysis. VRS training was more efficient than BT training (P < 0.00001, 95% CI: 35.08 to -25.01) when evaluating participant time needed to complete the peg task. In descriptive analysis, VRS training was better than BT training in participant performance score to perform MIS. There was no statistical difference in the meta-analysis in the time needed to perform surgery, time to complete basic or advanced tasks and performance score for basic or advanced tasks. Conclusions: VRS training was better than BT training in participant performance scores when performing MIS and in the time needed to complete the basic task of peg transfer. In all other outcomes, regardless of the student's level of experience or type of activity, the two forms of training were equivalent.
- Characterization of traumatic spinal cord injury model in relation to neuropathic pain in the rat(2019) BATISTA, Chary Marquez; MARIANO, Eric Domingos; ONUCHIC, Fernando; DALE, Camila Squarzoni; SANTOS, Gustavo Bispo dos; CRISTANTE, Alexandre Fogaca; OTOCH, Jose Pinhata; TEIXEIR, Manoel Jacobsen; MORGALLA, Matthias; LEPSKI, GuilhermePurpose/aim: Neuropathic pain following spinal cord injury (SCI) has a tremendous impact on patient's quality of life, and frequently is the most limiting aspect of the disease. In view of the severity of this condition and the absence of effective treatments, the establishment of a reliable animal model that reproduces neuropathic pain after injury is crucial for a better understanding of the pathophysiology and for the development of new therapeutic strategies. Thus, the objective of the present study was to standardize the traumatic SCI model in relation to neuropathic pain.Materials and methods: Wistar rats were submitted to SCI of mild intensity (pendulum height 12.5mm) or moderate intensity (pendulum height 25mm) using the New York University Impactor equipment. Behavioural assessment was performed during 8weeks. Thereafter, spinal cords were processed for immunohistochemistry.Results: The animals of the moderate injury group in comparison with mild injury had a greater motor function deficit, worse mechanical allodynia, and latter bladder recovery; moreover, histological analysis revealed more extensive lesions with lower neuronal population.Conclusions: Our study suggests that moderate SCI causes a progressive and long-lasting painful condition (at least 8weeks), in addition to motor impairment, and thus represents a reliable animal model for the study of chronic neuropathic pain after SCI.
conferenceObject Can Antisense Oligonucleotides Specific to K-Ras Gene Inhibit the Tumor Growth, Invasion and MMP-2 and MMP-9 Expression in Hamster Pancreatic Cancer Model In Vitro and In Vivo?(2013) MORIOKA, C. Y.; OTOCH, J. P.; SAITO, S.; MACHADO, M. C. C.; WATANABE, A.; HUANG, C. C.- Cancer as a Proinflammatory Environment: Metastasis and Cachexia(2015) PINTO, Nelson Inacio; CARNIER, June; OYAMA, Lila M.; OTOCH, Jose Pinhata; ALCANTARA, Paulo Sergio; TOKESHI, Flavio; NASCIMENTO, Claudia M.The development of the syndrome of cancer cachexia and that of metastasis are related with a poor prognostic for cancer patients. They are considered multifactorial processes associated with a proinflammatory environment, to which tumour microenvironment and other tissues from the tumour bearing individuals contribute. The aim of the present review is to address the role of ghrelin, myostatin, leptin, HIF, IL-6, TNF-alpha, and ANGPTL-4 in the regulation of energy balance, tumour development, and tumoural cell invasion. Hypoxia induced factor plays a prominent role in tumour macro-and microenvironment, by modulating the release of proinflammatory cytokines.
bookPart Sutura de lesões intestinais(2012) OTOCH, Jose Pinhata- A simplified experimental model of large-for-size liver transplantation in pigs(2013) LEAL, Antonio Jose Goncalves; TANNURI, Ana Cristina Aoun; BELON, Alessandro Rodrigo; GUIMARAES, Raimundo Renato Nunes; COELHO, Maria Cecilia Mendonca; GONCALVES, Josiane de Oliveira; SOKOL, Suellen Serafini; MELO, Evandro Sobroza De; OTOCH, Jose Pinhata; TANNURI, UenisOBJECTIVE: The ideal ratio between liver graft mass and recipient body weight for liver transplantation in small infants is unknown; however, if this ratio is over 4%, a condition called large-for-size may occur. Experimental models of large-for-size liver transplants have not been described in the literature. In addition, orthotopic liver transplantation is marked by high morbidity and mortality rates in animals due to the clamping of the venous splanchnic system. Therefore, the objective of this study was to create a porcine model of large-for-size liver transplantation with clamping of the supraceliac aorta during the anhepatic phase as an alternative to venovenous bypass. METHOD: Fourteen pigs underwent liver transplantation with whole-liver grafts without venovenous bypass and were divided into two experimental groups: the control group, in which the weights of the donors were similar to the weights of the recipients; and the large-for-size group, in which the weights of the donors were nearly 2 times the weights of the recipients. Hemodynamic data, the results of serum biochemical analyses and histological examination of the transplanted livers were collected. RESULTS: The mortality rate in both groups was 16.5% (1/7). The animals in the large-for-size group had increased serum levels of potassium, sodium, aspartate aminotransferase and alanine aminotransferase after graft reperfusion. The histological analyses revealed that there were no significant differences between the groups. CONCLUSION: This transplant method is a feasible experimental model of large-for-size liver transplantation.