LUIZ AUGUSTO CARNEIRO D ALBUQUERQUE
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Gastroenterologia, Faculdade de Medicina - Docente
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
LIM/37 - Laboratório de Transplante e Cirurgia de Fígado, Hospital das Clínicas, Faculdade de Medicina - Líder
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
LIM/37 - Laboratório de Transplante e Cirurgia de Fígado, Hospital das Clínicas, Faculdade de Medicina - Líder
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- Ischemic Preconditioning-Like Effect of Polyunsaturated Fatty Acid-Rich Diet on Hepatic Ischemia/Reperfusion Injury(2011) COELHO, Ana Maria Mendonca; MACHADO, Marcel Cerqueira Cesar; TAKAHASHI, Hilton Kenji; SAMPIETRE, Sandra N.; STEFANO, Jose Tadeu; LEITE, Andre Zonetti A.; CURI, Rui; D'ALBUQUERQUE, Luiz A. CarneiroAim The aim of this study was to investigate a possible preconditioning effect of oral diet enriched with polyunsaturated fatty acids (PUFAs) on liver ischemia/reperfusion (I/R) injuries. Methods Wistar male rats were fed a standard diet or polyunsaturated fatty acid-rich diet (PRD) enriched with (GII) or without (GIII) omega-3 PUFA. Rats were submitted to partial liver ischemia during 1 h and evaluated in pre- and post-I/R conditions. In pre-I/R condition, livers were collected for determination of fatty acid composition, liver mitochondrial function, malondialdehyde (MDA) content, and histological analysis. Four hours after liver reperfusion serum activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT), serum levels of tumor necrosis factor-alpha, interleukin-6, interleukin-10, and prostaglandin-E2, liver mitochondrial function, MDA content, and histology were evaluated. Results In the pre-I/R condition, GII and GIII groups had an increase on PUFA content and exhibited slight increased macrosteatosis and microsteatosis in the liver. After 4 h of reperfusion, PRD-fed rats showed a marked decrease on steatosis, diminished necrosis, an increase in MDA formation, and mitochondrial uncoupling. We also observed a marked decrease in plasma levels of cytokines and ALT and AST activities in post-I/R condition in PRD groups. Conclusion In this experimental model in the rat, PRD has a preconditioning effect protecting the liver from I/R injury and should be object of future clinical studies.
- The best approach for splenectomy in portal hypertension(2011) ANDRAUS, Wellington; PINHEIRO, Rafael S.; HADDAD, Luciana B.; HERMAN, Paulo; D'ALBUQUERQUE, Luiz Augusto C.
article 2 Citação(ões) na Scopus Critical analysis of the use of statistical tests in Brazilian publications related to digestive tract surgery(2011) ORSO, Ivan Roberto Bonotto; PEREIRA, Julio C. R.; D'ALBUQUERQUE, Luiz A. C.; CECCONELLO, Ivan; JUKEMURA, JoseObjective: To evaluate the evolution of scientific papers published on digestive tract surgery in Brazilian journals in 20 years and examine whether the level of evidence in the studies has improved, as well as the incorporation of statistical procedures and their proper application. Methods: We selected all original articles related to digestive tract surgery published in the years of 1987 and 2007 in 4 leading Brazilian surgical journals. Studies were divided according to the level of evidence (I: prospective, controlled and randomized, II: prospective without control or randomization and III: retrospective) and compared them to assess whether there was an improvement in the level of evidence between these two years surveyed. We also assessed whether there was increased use of analytical statistics and correct application of statistical procedures. Results: Comparing the articles published in 1987 with those of 2007, we observed no improvement with respect to the level of evidence, with more than half of the articles published being case series and retrospective studies (56.14%). There has been a significant increase in the use of analytical statistics (70.4% in 2007 vs. 40% in 1987) in the 20 years, however 16.7% of the studies published in 2007 did not correctly apply or adequately describe the statistical analyses used. Conclusion: In this study, we observed no improvement in the level of evidence presented in publications on digestive tract surgery in the last 20 years. There was an increased use of statistical analysis, but there is a need to correctly apply statistical procedures.- Graft-versus-host disease after liver transplantation(2011) CHAIB, Eleazar; SILVA, Felipe D.; FIGUEIRA, Estela R. R.; LIMA, Fabiana R.; ANDRAUS, Wellington; D'ALBUQUERQUE, Luiz Augusto C.
- The best approach for diagnosing primary sclerosing cholangitis(2011) ANDRAUS, Wellington; HADDAD, Luciana; NACIF, Lucas Souto; SILVA, Felipe D.; BLASBALG, Roberto; AUGUSTO, Luiz; D'ALBUQUERQUE, Carneiro
- Place of Liver Transplantation in the Treatment of Hepatocellular Carcinoma in the Normal Liver(2011) LERUT, Jan; MERGENTAL, Hynek; KAHN, Delawir; ALBUQUERQUE, Luiz; MARRERO, Juan; VAUTHEY, Jean Nicolas; PORTE, Robert J.
- Total Pancreatectomy: Porcine Model for Inducing Diabetes - Anatomical Assessment and Surgical Aspects(2011) CHAIB, E.; GALVAO, F. H. F.; ROCHA-FILHO, J. A.; SILVEIRA, B. L.; CHEN, L.; CARVALHO, M. P. D. de C.; PARIZ, C. E.; ALMEIDA, F. S. de; WAISBERG, D. R.; SOUZA, Y. E. D. M. de; MACHADO, M. C. C.; D'ALBUQUERQUE, L. A. C.Background: The swine is an essential model for carrying out preclinical research and for teaching complex surgical procedures. There is a lack of experimental models describing anatomical and surgical aspects of total pancreatectomy in the pig. Materials and Methods: The experiments were performed on 10 white male swine weighing 27-33 kg. The animals were premedicated with midazolam (0.4 mg/kg, i.m.) and ketamine (4 mg/kg, i.m.). Anesthesia was induced with propofol (1-2 mg/kg, i.v.) and was maintained with propofol and fentanyl (0.3 mg and 0.1 mu g/kg/min, respectively, i.v.). The surgical period ranged from 44 to 77 min. The pancreas anatomy, and the main arterial, venous and pancreatic duct anatomy were assessed. Results: The pancreas anatomy was composed of 3 lobes, the 'splenic', 'duodenal' and 'connecting' lobe which is attached to the anterior portion of the portal vein. The splenic artery and the junction of the splenic vein and portal vein were divided. The left gastric artery was dissected and separated from its origin at the splenic artery. The head of the pancreas is disposed in a C shape. The pancreas was dissected and liberated from the right portion of the portal vein and the infrahepatic vena cava. The pancreas was separated from the duodenum preserving the pancreaticoduodenal artery, then we performed the total pancreatectomy preserving the duodenum, common bile duct and spleen. Conclusion: Total pancreatectomy with duodenum, bile duct and spleen preservation in the pig is feasible and an important instrument for research purposes and teaching surgical technique.
article 2 Citação(ões) na Scopus Small bowel transplantation in outbred rats(2011) WAISBERG, Daniel Reis; LEE, Andre Dong Wong; SANTOS, Rafael Miyashiro Nunes dos; MORY, Eduardo Kenji; COSTA, Anderson Lino; MONTERO, Edna Frasson de Souza; CHAIB, Eleazar; D'ALBUQUERQUE, Luis Augusto Carneiro; GALVAO, Flavio Henrique FerreiraPURPOSE: To investigate the clinical evolution of orthotopic small bowel transplantation in outbred rats. METHODS: Seventy-two outbred Wistar rats weighting from 250 to 300g were used as donor and recipient in 36 consecutives ortothopic small intestine transplantation without immunosuppression. The graft was transplanted into the recipient using end-to-side aortic and portacaval microvascular anastomosis. Procedure duration, animal clinical course and survival were evaluated. Survival shorter than four days was considered technical failure. Recipients were sacrificed with signs of severe graft rejection or survival longer than 120 days. Necropsies were performed in all recipients to access histopathological changes in the graft. RESULTS: Median time for the procedure was 107 minutes. Six recipients (16.7%) presented technical failure. Twenty-seven recipients were sacrificed due to rejection, being nineteen (52.7%) between 7(th) and 15(th) postoperative day and eight (22.2%) between 34(th) and 47(th) postoperative day. Graft histology confirmed severe acute cellular rejection in those recipients. Uneventful evolution and survival longer than 120 days without rejection were observed in three recipients (8.3%). CONCLUSION: Intestinal transplantation in outbred rats without immunosuppressant regiment accomplishes variable clinical evolution.- INTESTINAL TRANSPLANTATION USING CUFF-GLUE SUTURELESS TECHNIQUE FOR MICROANASTOMOSIS IN RATS(2011) WAISBERG, Daniel Reis; GALVAO, Flavio Henrique Ferreira; GALVAO, Raoni De Castro; CHAIB, Eleazar; D'ALBUQUERQUE, Luiz Augusto Carneiro