CAROLINE SILVERIO FARIA
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
LIM/03 - Laboratório de Medicina Laboratorial, Hospital das Clínicas, Faculdade de Medicina
LIM/03 - Laboratório de Medicina Laboratorial, Hospital das Clínicas, Faculdade de Medicina
16 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 16
- Impact of Three Methods of Ischemic Preconditioning on Ischemia-Reperfusion Injury in a Pig Model of Liver Transplantation(2022) BELON, Alessandro Rodrigo; TANNURI, Ana Cristina Aoun; MOREIRA, Daniel de Albuquerque Rangel; FIGUEIREDO, Jose Luiz; SILVA, Alessandra Matheus da; SERAFINI, Suellen; GUIMARAES, Raimundo Renato; FARIA, Caroline Silverio; ALEXANDRE, Alcione Sanches de; GONCALVES, Josiane Oliveira; PAES, Vitor Ribeiro; TANNURI, UenisBackground Ischemic preconditioning (IPC), either direct (DIPC) or remote (RIPC), is a procedure aimed at reducing the harmful effects of ischemia-reperfusion (I/R) injury. Objectives To assess the local and systemic effects of DIPC, RIPC, and both combined, in the pig liver transplant model. Materials and methods Twenty-four pigs underwent orthotopic liver transplantation and were divided into 4 groups: control, direct donor preconditioning, indirect preconditioning at the recipient, and direct donor with indirect recipient preconditioning. The recorded parameters were: donor and recipient weight, graft-to-recipient weight ratio (GRWR), surgery time, warm and cold ischemia time, and intraoperative hemodynamic values. Blood samples were collected before native liver removal (BL) and at 0 h, 1 h, 3 h, 6 h, 12 h, 18 h, and 24 h post-reperfusion for the biochemical tests: aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), creatinine, BUN (blood urea nitrogen), lactate, total and direct bilirubin. Histopathological examination of liver, gut, kidney, and lung fragments were performed, as well as molecular analyses for expression of the apoptosis-related BAX (pro-apoptotic) and Bcl-XL (anti-apoptotic) genes, eNOS (endothelial nitric oxide synthase) gene, and IL-6 gene related to inflammatory ischemia-reperfusion injury, using real-time polymerase chain reaction (RT-PCR). Results There were no differences between the groups regarding biochemical and histopathological parameters. We found a reduced ratio between the expression of the BAX gene and Bcl-XL in the livers of animals with IPC versus the control group. Conclusions DIPC, RIPC or a combination of both, produce beneficial effects at the molecular level without biochemical or histological changes.
conferenceObject Evaluation of a biomarker panel for the diagnosis of cavity effusions(2018) ANTONANGELO, L.; FARIA, C. Silverio; ASCENCIO, M. M. P.; ROSOLEN, D. Cristina Batista; DOI, D.; FARIA, D. Kanaan- Response to: Necessity of co-operation between pulmonologists and internists in tuberculous pleurisy diagnosis(2019) ANTONANGELO, Leila; FARIA, Caroline S.; SALES, Roberta K.
- Dynamics and Heterogeneity of the Lung Immunopathology in Severe COVID-19(2022) ERJEFALT, J.; COSTA, N. De Souza Xavier; JONSSON, J.; COZZOLINO, O.; DANTAS, K.; CLAUSSON, C.; SIDDHURAJ, P.; LINDO, C.; LOMBARDI, S. Ferreira Spina; MENDRONI JUNIOR, A.; ANTONANGELO, L.; FARIA, C. Silverio; DUARTE NETO, A. Nunes; MONTEIRO, R. De Almeida; PINHO, J. Rebello; GOMES-GOUVEA, M. Soares; PEREIRA, R. Verciano; MONTEIRO, J. Sirino; SETUBAL, J.; OLIVEIRA, E. Pierre De; THEODORO FILHO, J.; SANDEN, C.; ORENGO, J.; SLEEMAN, M.; SILVA, L. Ferraz Da; SALDIVA, P. Nascimento; DOLHNIKOFF, M.; MAUAD, T.
conferenceObject Performance of the UroVysion (R) FISH assay for the diagnosis of malignant effusions using two cutoff strategies(2018) ANTONANGELO, L.; FARIA, D. Kanaan; FARIA, C.; ROSOLEN, D. Cristina BatistaconferenceObject conferenceObject Genomic profile of primary non-small cell lung cancer and matched mediastinal lymph nodes by next-generation sequencing: a pilot study(2022) ANTONANGELO, L.; FARIA, C. S.; TERRA, R. M.; NASCIMENTO, E. C. T. do; MELLO, E. S. de; MANGONE, F. R. R.; NAGAI, M. A.; AGATI, M. E. M.; CAPELOZZI, V. L.conferenceObject Usefulness of type V collagen and alpha 2 beta 1-integrin in the cytological diagnosis of pleural liquid biopsy(2019) ANTONANGELO, L.; TEODORO, W. Rosolia; FARIA, C. Silverio; QUEIROZ, Z. A.; SILVEIRA, L. Ramos da; VELOSA, A. P.; CAPELOZZI, V.- Tuberculous pleural effusion: diagnosis & management(2019) ANTONANGELO, Leila; FARIA, Caroline S.; SALES, Roberta K.Background: Tuberculosis (TB) is the world's leading cause of death from infectious disease. The World Health Organization (WHO) recognized 6.3 million new TB cases in 2017, 16% corresponding to extrapulmonary forms; pleural tuberculosis (PT) is the most common extrapulmonary form in adults. PT diagnosis is often challenging because the scarcity of bacilli in pleural fluid (PF), sometimes requiring invasive procedures to obtain pleural tissue for histological, microbiological or molecular examination. In regions of medium and high disease prevalence, adenosine deaminase (ADA), interferon gamma (IFN-gamma) and interleukin 27 (IL-27) dosages are useful to establish presumptive diagnosis in patients with compatible clinical/radiological picture who present with lymphocytic pleural effusion. PT treatment is similar to the pulmonary TB treatment regimen recommended by WHO. Area covered: In this update, we present a PT review, including epidemiology, pathogenesis, clinical features, diagnosis, and therapy. Expert opinion: There is no PF test alone accurate for PT diagnosis, despite the evolution in clinical laboratory. ADA, IFN-gamma and IL-27 are valuable laboratory biomarkers; however, IFN-gamma and IL-27 are quite expensive. Molecular tests present low sensitivity in PF, being useful for diagnostic confirmation. Multidrug therapy remains the PT treatment choice. Advancing research in immunotherapy may bring benefits to PT patients.