MARIA CRISTINA CARVALHO DO ESPIRITO SANTO
Projetos de Pesquisa
Unidades Organizacionais
LIM/06 - Laboratório de Imunopatologia da Esquistossomose e outras Parasitoses, Hospital das Clínicas, Faculdade de Medicina
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conferenceObject DETECTION OF SCHISTOSOMA MANSONI SPOROCYST STAGE IN BIOMPHALARIA GLABRATA MOLLUSK IN EXPERIMENTAL CONDITIONS(2018) CASOTTI, Marcia; TUAN, Roseli; GOMES, Michele; LUNA, Expedito Albuquerque; DIAS-NETO, Emmanuel; PAULA, Fabiana; PINHO, Joao Rebello; CARRILHO, Flair; GRYSCHEK, Ronaldo Borges; ESPIRITO-SANTO, MariaconferenceObject MOLECULAR CHARACTERIZATION OF THE LARVAL PHASE OF SCHISTOSOMA MANSONI IN BIOMPHALARIA GLABRATA MOLLUSKS UNDER EXPERIMENTAL CONDITIONS(2017) CASOTTI, Marcia Oliveira; TUAN, Roseli Tuan; GOMES, Michele; DIAS-NETO, Emmanuel; PINHO, Joao Renato Rebello; PAULA, Fabiana Martins; CARRILHO, Flair Jose Carrilho Jose; LUNA, Expedito Jose Albuquerque; GRYSCHEK, Ronaldo Cesar Borges Borges; ESPIRITO-SANTO, Maria Cristina- TWO SEQUENTIAL PCR AMPLIFICATIONS FOR DETECTION OF Schistosoma mansoni IN STOOL SAMPLES WITH LOW PARASITE LOAD(2012) ESPIRITO-SANTO, Maria Cristina Carvalho do; ALVARADO-MORA, Monica Viviana; PINTO, Pedro Luiz Silva; CARRILHO, Flair Jose; PINHO, Joao Renato Rebello; GRYSCHEK, Ronaldo Cesar BorgesSchistosomiasis constitutes a major public health problem, with an estimated 200 million individuals infected worldwide and 700 million people living in risk areas. In Brazil there are areas of high, medium and low endemicity. Studies have shown that in endemic areas with a low prevalence of Schistosoma infection the sensitivity of parasitological methods is clearly reduced. Consequently diagnosis is often impeded due to the presence of false-negative results. The aim of this study is to present the PCR reamplification (Re-PCR) protocol for the detection of Schistosoma mansoni in samples with low parasite load (with less than 100 eggs per gram (epg) of feces). Three methods were used for the lysis of the envelopes of the S. mansoni eggs and two techniques of DNA extraction were carried out. Extracted DNA was quantified, and the results suggested that the extraction technique, which mixed glass beads with a guanidine isothiocyanate/phenol/chloroform (GT) solution, produced good results. PCR reamplification was conducted and detection sensitivity was found to be five eggs per 500 mg of artificially marked feces. The results achieved using these methods suggest that they are potentially viable for the detection of Schistosoma infection with low parasite load.
conferenceObject REVALENCE OF SCHISTOSOMA MANSONI INFECTION AND OTHER PARASITIC DISEASES IN PERIPHERAL AREAS OF BARRA MANSA, RIO DE JANEIRO, BRAZIL(2017) ESPIRITO-SANTO, Maria Cristina C.; CHIEFFI, Pedro Paulo; PAULA, Fabiana Martins de; CASTILHO, Vera Lucia Pagliusi; GONCALVES, Elenice Messias do Nascimento; ORBAN, Magali; PINHO, Joao Renato Rebello; LUNA, Expedito Jose de Albuquerque; GRYSCHEK, Ronaldo Cesar BorgesconferenceObject GENETIC DIVERSITY OF BLASTOCYSTIS SUBTYPES IN PATIENTS WITH CHRONIC URTICARIA(2017) PAULA, Fabiana M.; MELO, Gessica B.; MALTA, Fernanda M.; MARUTA, Celina W.; CRIADO, Paulo R.; CASTILHO, Vera Lucia P.; GONCALVES, Elenice M. N.; SANTO, Maria Cristina Espirito; GRYSCHEK, Ronaldo CesarconferenceObject CLINICAL, LABORATORY AND EPIDEMIOLOGICAL PROFILE AND TREATMENT OF SEVERE SCHISTOSOMIASIS MANSONI IN A TEACHING HOSPITAL IN SAO PAULO CITY, BRAZIL(2015) MAGALHAES, Maira Reina; ESPIRITO-SANTO, Maria Cristina Carvalho; MORTARI, Naima; LUNA, Expedito Jose; GRYSCHEK, Ronaldo CesarconferenceObject DIFFERENTIAL EXPRESSION OF MEMBRANE AND MEMBRANE-BOUND PROTEINS FROM FILARIFORM LARVAE AND ADULT FEMALE OF STRONGYLOIDES VENEZUELENSIS(2017) PAULA, Fabiana M.; CORRAL, Marcelo A.; MARQUES, Priscilla D.; MEISEL, Dirce Mary C.; COSTA-CRUZ, Julia Maria; SANTO, Maria Cristina Espirito; CAMPOS, Jonatan M.; MATTEI, Bruno; BORGES, William Castro; GRYSCHEK, Ronaldo Cesar- Molecular detection of prepatent Schistosoma mansoni infection in Biomphalaria glabrata snail vectors(2020) CASOTTI, Marcia Oliveira; GRYSCHEK, Ronaldo Cesar Borges; PAULA, Fabiana Martins de; GOMES-GOUVEA, Michele; PINHO, Joao Renato Rebello; TUAN, Roseli; DIAS-NETO, Emmanuel; LUNA, Expedito Jose de Albuquerque; ESPIRITO-SANTO, Maria Cristina Carvalho doApproximately 240 million people worldwide are infected by Schistosoma. In Brazil, one of the main intermediate hosts of this parasite is Biomphalaria glabrata snails. The early detection of larval stages in intermediate hosts is an important challenge to public health. but it also represents an opportunity as a new alternative to indicate earlier natural infections before cercariae differentiation and emergence. In this context, we demonstrated that PCR amplification of a 28S gene fragment from the parasite does demonstrate S. mansoni infection in snails 14 days post infection. This conventional polymerase chain reaction amplified clear bands and was able to detect parasitic infection in the intermediate host B. glabrata under experimental conditions. However, we reinforce that this approach requires deeper investigations and further comparisons to confirm its specificity and sensitivity in earlier time points after miracidia infection. This approach has relevant potential as an effective molecular-based strategy for the monitoring of schistosomiasis transmission.
- Access to drinking water and sewage treatment in Brazil: a challenge for the control of waterborne infectious diseases(2020) GOMES, Filumena Maria da Silva; SANTO, Maria Cristina Carvalho do Espirito; GRYSCHEK, Ronaldo Cesar Borges; BERTOLOZZI, Maria Rita; FRANCA, Francisco Oscar de Siqueira
- THE FIRST CASE OF Angiostrongylus cantonensis EOSINOPHILIC MENINGITIS DIAGNOSED IN THE CITY OF SAO PAULO, BRAZIL(2013) ESPIRITO-SANTO, Maria Cristina Carvalho do; PINTO, Pedro Luiz Silva; MOTA, Dan Jesse Goncalves da; GRYSCHEK, Ronaldo Cesar BorgesIntroduction: Angiostrongylus cantonensis is a natural parasite found in lung arteries of rats, which in humans may cause eosinophilic meningitis. Objective: To report the first case of eosinophilic meningitis caused by Angiostrongylus cantonensis in the city of Sao Paulo, Brazil. Case report: A male patient, 11 years old, living in the southern area of Sao Paulo, was admitted to the Pediatric Emergency Department with ongoing headaches for three days, but no fever or any other complaint. The presence of snails and rodents was reported in the peridomicile. The child was awake, lucid, oriented; muscular strength preserved, isochoric, photo reagent pupils and terminal nuchal rigidity - Glasgow Coma Scale (GCS) = 15. The laboratory tests showed a mild leukocytosis with 1736 eosinophils/mm(3) and the CSF analysis disclosed 160 leukocytes/mm(3) with 36% of eosinophils. The bacterial culture was negative. Computed Cerebral Tomography showed no alterations. The RT-PCR assay for detecting Angiostrongylus cantonensis larvae and DNA was negative. ELISA antibodies for IgG anti-A. cantonensis was negative in serum and undetermined in CSF and samples collected five days after the onset of symptoms. Seroconversion was observed in the sample collected 135 days later. Conclusion: the epidemiological and clinical data, the CSF alterations with eosinophilia and the seroconversion strongly suggest Angiostrongylus cantonensis eosinophilic meningitis.