BEATRIZ MANGUEIRA SARAIVA RAMANHOLO
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
LIM/20 - Laboratório de Terapêutica Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/20 - Laboratório de Terapêutica Experimental, Hospital das Clínicas, Faculdade de Medicina
3 resultados
Resultados de Busca
Agora exibindo 1 - 3 de 3
- Low-dose chlorine exposure impairs lung function, inflammation and oxidative stress in mice(2021) GENARO, Isabella Santos de; ALMEIDA, Francine Maria de; LOPES, Fernanda Degobbi Tenorio Quirino dos Santos; KUNZLER, Deborah De Camargo Hizume; TRIPODE, Bruna Gabryela Busoletto; KURDEJAK, Adriana; CORDEIRO, Bruna Nakamura; PANDOLPHO, Renata; MACCHIONE, Mariangela; BRUGGEMANN, Thayse Regina; VIEIRA, Rodolfo Paula; MARTINS, Milton Arruda; TIBERIO, Iolanda de Fatima Lopes Calvo; SARAIVA-ROMANHOLO, Beatriz MangueiraAim: To explore the different consequences of acute and chronic exposure to chlorine gas (Cl-2) on the functional and histological parameters of health mice. Main methods: Firstly, male BALB/c mice were acute exposed to 3.3 or 33.3 or 70.5 mg/m(3) Cl-2. We analyzed the lung function, the inflammatory cells in the bronchoalveolar lavage, cell influx in the peribrochoalveolar space and mucus production. In a second phase, mice were chronic exposed to 70.5 mg/m(3) Cl-2. Besides the first phase analyses, we also evaluated the epithelial cells thickness, collagen deposition in the airways, immunohisto-chemistry stain for IL-1 beta, iNOS, IL-17 and ROCK-2 and the levels of IL-5, IL-13, IL-17, IL-1 beta and TNF-alpha in lung homogenate. Key findings: Acute exposure to chlorine impaired the lung function, increased the number of inflammatory cells in the BALF and in the airways, also increased the mucus production. Furthermore, when chlorine was exposed chronically, increased the airway remodeling with collagen deposition and epithelial cells thickness, positive cells for IL-1 beta, iNOS, IL-17 in the airways and in the alveolar walls and ROCK-2 in the alveolar walls, lung inflammation with increased levels of IL-5, IL-13, IL-1 beta and TNF-alpha in the lung homogenate, and also, induced the acid mucus production by the nasal epithelium. Significance: Acute and chronic exposure to low dose of chlorine gas worsens lung function, induces oxidative stress activation and mucus production and contributes to augmenting inflammation in health mice.
- Comparison of cardiac autonomic modulation of athletes and non-athletes individuals with spinal cord injury at rest and during a non-immersive virtual reality task(2021) DIAS, Eduardo Dati; MENEZES, Lilian Del Ciello de; SILVA, Talita Dias da; SILVA, Nadja Moreira da; VIDAL, Pamela Reis; BRONDANE, Bruno Renan; PADULA, Natalia; GASPAR, Roberta Caveiro; SANTOS, Sileno; AURICCHIO, Jose Ricardo; MONTEIRO, Carlos Bandeira de Mello; DOMINGO, Antoinette; OLIVEIRA, Camila Quel de; MACEDO, Jacqueline Carla de; ROMANHOLO, Beatriz Mangueira Saraiva; BARNABE, VivianiStudy design Cross-sectional study. Objectives To compare cardiac autonomic modulation of individuals with spinal cord injury (SCI) that practice different amounts of moderate to vigorous physical activity (PA) and able-bodied controls at rest and during a non-immersive Virtual Reality task. Setting Athletes with SCI of wheelchair basketball, wheelchair tennis, wheelchair handball, WCMX (wheelchair motocross), and para-swimming were assessed at the Faca na Cadeira Institute, ICEL and Clube Esperia in Sao Paulo, Brazil; non-athletes with SCI and able-bodied controls were assessed at the Acreditando Centro de Recuperacao Neuromotora, Sao Paulo, Brazil. Methods One-hundred forty-five individuals were assessed: 36 athletes with traumatic SCI (41.1 +/- 16.8 years old), 52 non-athletes with traumatic SCI (40.2 +/- 14.1 years old), and 57 able-bodied individuals (39.4 +/- 12.5 years old). Cardiac autonomic modulation was assessed through heart rate variability (HRV) measured in the sitting position at rest and during a VR game activity. Results We found significantly more favourable HRV for athletes with SCI when compared to non-athletes with SCI, but no differences between athletes with SCI and able-bodied controls. In addition, athletes and able-bodied controls showed adequate autonomic nervous system (ANS) adaptation (rest versus physical activity in VR), i.e., they experienced parasympathetic withdrawal during VR physical activity, which was not found in non-athletes with SCI. Conclusion The practice of moderate to vigorous physical activity is associated with healthier cardiac autonomic modulation in adults with SCI, which may lead to more favourable health outcomes.
- Cigarette smoke dissociates inflammation and lung remodeling in OVA-sensitized and challenged mice(2012) HIZUME, Deborah C.; TOLEDO, Alessandra C.; MORIYA, Henrique T.; SARAIVA-ROMANHOLO, Beatriz M.; ALMEIDA, Francine M.; ARANTES-COSTA, Fernanda M.; VIEIRA, Rodolfo P.; DOLHNIKOFF, Marisa; KASAHARA, David Itiro; MARTINS, Milton A.We evaluated the effects of cigarette smoke (CS) on lung inflammation and remodeling in a model of ovalbumin (OVA)-sensitized and OVA-challenged mice. Male BALB/c mice were divided into 4 groups: non-sensitized and air-exposed (control); non-sensitized and exposed to cigarette smoke (CS), sensitized and air-exposed (OVA) (50 mu g + OVA 1% 3 times/week for 3 weeks) and sensitized and cigarette smoke exposed mice (OVA + CS). IgE levels were not affected by CS exposure. The increases in total bronchoalveolar fluid cells in the OVA group were attenuated by co-exposure to CS, as were the changes in IL-4, IL-5, and eotaxin levels as well as tissue elastance (p < 0.05). In contrast, only the OVA + CS group showed a significant increase in the protein expression of IFN-gamma, VEGF, GM-CSF and collagen fiber content (p < 0.05). In our study, exposure to cigarette smoke in OVA-challenged mice resulted in an attenuation of pulmonary inflammation but led to an increase in pulmonary remodeling and resulted in the dissociation of airway inflammation from lung remodeling.