JULIO MORAIS BESTEIRO
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Cirurgia, Faculdade de Medicina - Docente
4 resultados
Resultados de Busca
Agora exibindo 1 - 4 de 4
- Light-emitting diode stimulates radiodermatitis recovery(2021) CAMARGO, Cristina Pires; CARVALHO, Heloisa Andrade; MALUF, Feres Camargo; SOUSA, Alexandre Agostinho da Cruz; PERIN, Paulo Otavio Maluf; PERIN, Marcela Maluf; MORAIS-BESTEIRO, Julio; GEMPERLI, RolfPurpose: To evaluate the effect of light-emitting diode (LED) in an experimental mod& of radiodermatitis. Methods: Ten male Wistar rats weighing 200-250 g were analyzed. Radiation was delivered in a single dose (20 Gy with Strontium-90 dermatological plaques), two areas per animal. After 15 days, they were divided into two groups: control group (n =5) and LED group (n =5), which was treated during 21 days later (LED 660 nm, 10 min in alternate days). The endpoints were radiodermatitis scale, histological analysis HE, Picrius Sirius and the gene expression of interleukin-10 (IL-10) and matrix metalloproteinase-9 (MMP-9). Results: The LED group showed a higher number of dermal appendages (p = 0.04) and angiogenesis (p = 0.007), a tendency towards higher IL-10 (p = 0.06) and an increase in MMP-9 (p = 0.004) when compared to the control group. Conclusion: This study suggested that the use of LED for radiodermatitis increased skin regeneration.
- Hyaluronic acid in tobacco-exposed rats. Inflammatory reaction, and duration of effects(2019) CAMARGO, Cristina Pires; FRASSEI, Renan Dias; SILVA, Daniel Imbassahy de Sa Bittencourt Camara e; PFANN, Robert Zawadzki; SILVA, Luiza de Campos Moreira da; MORAIS-BESTEIRO, Julio; GEMPERLI, RolfPurpose: To evaluate the hyaluronic acid (HA) inflammatory reaction, fibroblasts, fibrosis and duration of effect in the dorsal region of tobacco-exposed rats. Methods: Ten Wistar rats were divided into two groups: tobacco-exposed-group (TEG;n=5) and air-control-group (CG;n=5). The TEG animals were tobacco-exposed twice a day, 30-minutes/session, during 60 days. After this period, all animals received 0.1 mL HA subcutaneous injection in the dorsal area. The volume of HA was measured immediately after HA injection and weekly using a hand-caliper in nine weeks. After this period, all the animals were euthanized, and a specimen of was collected to evaluate inflammatory cells, fibroblasts, and fibrosis by HE. Results: This study showed a higher inflammatory reaction in TEG than CG: inflammatory cell-count (CG: 1.07 +/- 0.9; TEG: 8.61 +/- 0.36, p<0.001); fibroblast count (CG: 2.92 +/- 0.17; TEG: 19.14 +/- 0.62, p<0.001), and fibrosis quantification (CG: 2.0; TEG: 3.75, p<0.001). The analysis of the HA volume in nine weeks in the dorsal region did not show a difference between groups (p=0.39). Conclusions: This study suggested that the HA injection in the TEG caused an increase in inflammatory cell count, fibroblast, and fibrosis quantification when compared to the CG. There was no difference in the duration of effect of HA between the groups.
- The influence of adipocyte-derived stem cells (ASCs) on the ischemic epigastric flap survival in diabetic rats(2021) CAMARGO, Cristina Pires; KUBRUSLY, Marcia Saldanha; MORAIS-BESTEIRO, Julio; HARMSEN, Martim Conrad; GEMPERLI, RolfPurpose: To assess the effects of adipocyte-derived stem cell (ASC)-injection on the survival of surgical flaps under ischemia in diabetic rats. Methods: Diabetes was induced in 30 male Wistar rats using streptozotocin (55 mg/kg). After eight weeks, epigastric flap (EF) surgery was performed. The animals were divided into control (CG), medium-solution (MG), and ASC groups. The outcomes were: the survival area (SA), the survival/total area rate (S/TR), and expression levels (EL) of genes: C5ar1, Icam1, Nos2, Vegf-a. Results: In the ASC group, compared to CG, we observed improved flap SA (CG-420 mm(2) vs. ASC-720 mm(2); p=0.003) was observed. The S/TR analysis was larger in the ASC group (78%) than the CG (45%). This study showed an increase in the Vegf-a EL in the ASC group (2.3) vs. CG (0.93, p=0.0008). The Nos2 EL increased four-fold in the ASC group compared to CG, and C5ar1 EL decreased almost two-fold in the ASC group vs. the CG (p=0.02). There was no difference among the groups regarding Icam1 EL. Compared to the MG, the ASC group had a bigger flap SA (720 mm(2) vs. 301 mm(2), respectively), a bigger S/TR (78% vs. 32%, p=0.06, respectively) and increased EL of Vegf-a (2.3 vs. 1.3, respectively). No difference between ASC-group and MG was seen regarding Nos2 (p=0.08) and C5ar1 (p=0.05). Conclusion: This study suggests that ASCs increase the survival of EF under IR in diabetic rats.
- The positive effect of Botulinum toxin type A on the viability of random flap in tobacco exposed in rats(2016) CAMARGO, Cristina Pires; FERNANDES, Felipe Alexandre; LEE, Michel Hyo Mi; SILVA, Luiza Campos Moreira; BESTEIRO, Julio Morais; GEMPERLI, RolfPURPOSE: To evaluate the effect of Botulinum Toxin A in different time of tobacco exposure. METHODS: 60 male, Wistar rats were divided into two tobacco exposure groups: a 2-month or a 4-month regimen. After this period, these two groups were subdivided as two: saline solution(SS) or botulinum toxin A(Bonta), at the time of the surgery. Seven days before the SS or Bonta injection, the animals were submitted to a random flap (3x10cm). On the seventh postoperative day, all animals were assessed for total flap area, viable area, and the viable/total area ratio. RESULTS: This study showed a difference between groups 2-month saline vs. BontA injection (p=0.04); groups 4-month saline vs. BontA injection (p=0.001); groups 2-month saline vs. 4-month BontA (p=0.003), and, between groups 2-month BontA vs. 4-month saline(p=0.03). CONCLUSIONS: Botulinum Toxin A increased random flap viability in tobacco-exposed rats. Two months of tobacco exposure had the same effect as exposure for four months.