FABIANO INACIO DE SOUZA
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LIM/41 - Laboratório de Investigação Médica do Sistema Músculoesquelético, Hospital das Clínicas, Faculdade de Medicina
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article 11 Citação(ões) na Scopus SPINAL CORD INJURY EXPERIMENTAL MODEL AND MOTION EVALUATION PROTOCOL IN WISTAR RATS(2011) SANTOS, Gustavo Bispo dos; CRISTANTE, Alexandre Fogaca; MARCON, Raphael Martus; SOUZA, Fabiano Inacio de; BARROS FILHO, Tarcisio Eloy Pessoa de; DAMASCENO, Marcelo LoquetteObjectives: To determine a standardized spinal cord contusion model and a method for motor assessment in rats with paraplegia. Methods: This study used 20 Wistar rats divided into 4 groups according to level of severity of spinal cord injury; standardized intermediate lesions were made through system MASCIS IMPACTOR (Multicenter Animal Spinal Cord Injury Study): group 1, 12.5mm (mild injury); group 2,25mm (moderate injury); group 3,50mm (severe injury; in the group 4 the animals suffered no injury (control group). Motor function was assessed after 48 hours, using the scale proposed by Basso, Beattie and Bresnahan. Results: Using the model, we observed that the mild contusions (12.5mm height) were effective, and the animals presented acute urinary tract infection one week after the injury. Moderate contusions (25mm height) were effective, and the animals presented urinary infection until 2 weeks after injury. The severe contusions (50mm height) were effective, and the animals presented urinary infection for 3 to 4 weeks and autophagy. Conclusion: The model of spinal cord injury using the system MASCIS IMPACTOR and the functional assessment proposed by Basso, Beattie and Bresnahan is reproducible and can be used, enabling information exchange among different researchers.