ARIANA CAMPOS YANG
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina - Médico
LIM/60 - Laboratório de Imunologia Clínica e Alergia, Hospital das Clínicas, Faculdade de Medicina
LIM/60 - Laboratório de Imunologia Clínica e Alergia, Hospital das Clínicas, Faculdade de Medicina
14 resultados
Resultados de Busca
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conferenceObject Clinical evolution of Kaposi's varicelliform eruption using Immunosuppressive drugs as treatment for atopic dermatitis(2020) PACHANI, M. A. D. S.; ZANETTI, F. A.; BIALOWAS, D.; NUNES, S. A.; LOPES, M. M.; MARTINS, C. R. S.; CASTRO, F. F. M.; KALIL, J.; YANG, A. C.- Eosinophilic Esophagitis: Latent Disease in Patients with Anaphylactic Reaction to Cow's Milk(2018) BARBOSA, Adriana C.; CASTRO, Fabio Morato; MEIRELES, Paula Rezende; ARRUDA, L. Karla; CARDOSO, Silvia Regina; KALIL, Jorge; YANG, Ariana C.BACKGROUND: Food allergy and eosinophilic esophagitis are a substantial and evolving public health issue. Clinicians should know the relationship between these diseases and how one may predispose to the other. This can help minimize misdiagnosis. OBJECTIVE: The objective of this study was to assess esophageal eosinophilia and eosinophilic esophagitis frequency in patients with persistent cow's milk allergy and anaphylaxis manifestations. METHODS: Patients with persistent cow's milk allergy with anaphylaxis manifestations were enrolled from 2012 through 2016 at the Sao Paulo University Hospital, Brazil. All of them were submitted to endoscopy despite the presence or absence of gastrointestinal symptoms. Demographics data, atopic comorbidities, medication use, endoscopic findings, and esophageal eosinophilia frequency were evaluated. RESULTS: Eighty-nine patients were selected. The median age was 8 years. It was observed that 34 of 89 patients (38.2%; 95% confidence interval [CI]: 28.14%-49.16%) presented with esophageal eosinophilia. Five patients (7.1%) presented proton pump inhibitor-responsive esophageal eosinophilia, and 10 patients (14.2%) presented eosinophilic esophagitis. We found that 29.4% were asymptomatic patients, 23.5% had nonspecific symptoms, 23.5% had persistent typical symptoms, and 23.5% had intermittent typical symptoms. There was an association with inflammatory endoscopy findings in 21 patients (61.7%). CONCLUSIONS: This description demands scientific attention because it is the highest frequency of esophageal eosinophilia yet described in a group of patients with cow's milk allergy presenting with anaphylaxis. Eosinophilic esophagitis is a condition that can coexist ""silently"" with an IgE-mediated food allergy and is most often underestimated and under-diagnosed. (C) 2017 American Academy of Allergy, Asthma & Immunology
- Novel allergens from ancient foods: Man e 5 from manioc (Manihot esculenta Crantz) cross reacts with Hev b 5 from latex(2013) SANTOS, Keity Souza; GADERMAIER, Gabriele; VEJVAR, Eva; ARCURI, Helen Andrade; GALVAO, Clovis Eduardo; YANG, Ariana Campos; RESENDE, Virginia Maria Ferreira; MARTINS, Carlo de Oliveira; HIMLY, Martin; MARI, Adriano; LISO, Marina; POMPONI, Debora; BREITENEDER, Heimo; WAGNER, Stefan; KALIL, Jorge; FERREIRA, Fatima; CASTRO, Fabio Fernandes MoratoScope Manioc (Manihot esculenta) is a tuber mainly consumed in the Southern Hemisphere and used worldwide by food and chemistry industry. We aimed to recombinantly produce and characterize the first manioc allergen and evaluate its IgE reactivity in sera of Brazilian and Italian patients. Methods and results The molecule, termed Man e5, was expressed in E. coli, characterized by amino acid analysis, mass spectrometry, circular dichroism, HPLC, and dynamic light scattering. A tertiary structural model of the protein was produced using bioinformatics and susceptibility to pepsin digestion was analyzed in vitro. Based on its high content of charged residues, heat stability, flexibility and lack of secondary structure elements, the allergen was determined a member of the intrinsically disordered protein family. Brazilian patients were selected based on manioc allergy and Italians based on latex allergy and sensitization to Hev b 5.71% of Brazilians and 40% of Italians were in vitro IgE positive to Man e5. Cross-inhibition assays suggest a possible involvement of this allergen in the latex-fruit syndrome. Conclusion Man e5, the first purified allergen from manioc demonstrates IgE cross-reactivity with Hev b 5. Data suggest Hev b 5 might act as primary sensitizer and could therefore lead to allergic manifestations upon manioc consumption without prior exposition.
conferenceObject Safety to Extensively Heated Cow's Milk Challenge in Children with Severe Cow's Milk Allergy(2017) CORDOVA, Pablo Torres; PEDROSO, Natalia Falci; MELLO, Marcella Fonseca; SALLES-CUNHA, Patricia; KALIL, Jorge; CASTRO, Fabio M.; YANG, Ariana Campos- Allergic reactions to manioc (Manihot esculenta Crantz): Identification of novel allergens with potential involvement in latex-fruit syndrome(2011) SANTOS, Keity Souza; GALVAO, Clovis Eduardo; GADERMAIER, Gabriele; RESENDE, Virginia Maria Ferreira; MARTINS, Carlo de Oliveira; MISUMI, Denise Shimbo; YANG, Ariana Campos; FERREIRA, Fatima; PALMA, Mario Sergio; KALIL, Jorge; CASTRO, Fabio Fernandes Morato
conferenceObject Food consumption and nutritional status evaluation of children with atopic dermatitis(2012) PENTERICH, V; YANG, A.; PEREIRA, R.; TAKAYAMA, L.; KALIL, J.; CASTRO, F.Background: Atopic dermatitis (AD) is an inflammatory allergic skin disease that often requires glucocorticosteroid therapy. Due to recurrent association with food allergy, dietary intake of these children might be an important concern. The aim of this study was to evaluate food ingestion and nutritional status in children with moderate to severe AD compared with a control group. Method: We evaluated 60 children with atopic dermatitis and 54 healthy controls (HC), aged from 4 to 12 years old. Food ingestion was evaluated within 3 days 24-h food recalls. The dietary intake was evaluated according to the standards set by the American Institute of Medicine (2010), Dietary References Intake – DRIs, for calcium and vitamin D, and by the National Academy of Sciences Research Council (1989), named Recommended Dietary Allowance – RDA, for proteins and calories. The nutritional status was evaluated according to height to age z-score, weight for age z-score and body mass index (BMI) z-score, folowing the World Health Organization child growth standards (2006). Result: Results are described in Table 1. Dietary intake evaluation showed a tendency of less consumption of calories in the AD group ( P = 0.061), although both groups had an adequate consumption of calories. It was interesting to notice that in both groups there was an exceeding amount of proteins consumed compared to the standard recommendations, even though there was no statistical difference between groups. There was a statistically significant difference considering fat consumption (P< 0.05), children with AD consumed more fat than HC. Most of the AD patients consumed <80% of the dietary recommendations for calcium and vitamin D. In the subgroup of children who had also cow’s milk allergy the consumption of calcium was lower than 27%. Children with AD were smaller than HC children, according to the height for age z- score for nutritional status evaluation. Conclusion: Children with AD had a high ingestion of proteins and a low consumption of calcium and vitamin D. The AD children had a decrease in the Z-score of height for age.conferenceObject Endoscopic patterns and complications of eosinophilic esophagitis(2020) GOMES, L. S.; CORDOVA, P. T.; MAMEDE, L. Q.; PEREIRA, G. F.; SOUZA, A. B.; KALIL, J.; CASTRO, F. F. M.; YANG, A. C.- Underlying IPEX syndrome in a patient with idiopathic juvenile arthritis and vitiligo(2022) MENDONCA, Leonardo Oliveira; CHUSTER, Adriana Pitchon dos Reis; DORNA, Mayra Barros; BARROS, Samar Freschi; ALVES, Janaina Baptista; GONCALVES, Victor Lucas; YANG, Ariana Campos; KALIL, Jorge; TOLEDO-BARROS, Myrthes Anna Maragna; KOKRON, Cristina MariaBackground: IPEX syndrome is an X-linked inborn error of immunity clinically characterized by the triad of: enteropathy, polyendocrinopathy and eczema. However many other clinical presentations lacking the triad above described have been reported what underpin the need of careful clinical suspicion, immunological evaluation and genetic sequencing. Case presentation: Here we report a case of a Brazilian boy with severe eczema as the first and only presentation requiring cyclosporin therapy. Progressive and cumulative symptoms of arthritis and enteropathy lead to the suspicion of an inborn error of immunity. Peripheral FOXP3 expression was normal (CD127-/CD4+/CD25+/FOXP3+-396 cells-63%) and a pathogenic mutation in FOXP3 gene (c.1150G > A; p.Ala384Thr), confirmed the diagnosis of IPEX syndrome. Conclusions: IPEX syndrome should be suspected in patients presenting with severe eczema associated or not with other autoimmune/hyper inflammatory diseases in life. Our study also reinforces that FOXP3 expression by flowcytometry seems not to be a good screening method, and genetic sequencing is mandatory even in those with high suspicion and normal peripheral FOXP3 expression.
conferenceObject Cross-Reactivity Among Cereal Grains(2016) MENDONCA, Juliana Guimaraes; CASTRO, Roberta Almeida; CORDOVA, Pablo Torres; MEIRELES, Paula Rezende; FIGO, Daniele Danella; SANTOS, Keity Souza; KALIL, Jorge; CASTRO, Fabio Fernandes Morato; YANG, Ariana C.conferenceObject Severity profile of cow milk protein allergy IgE mediated(2017) ANAGUSKO, Claudia Leiko Yonekura; LOPES, Mariele Morandin; CAU, Larissa Prando; MARQUES, Paula Quadros; SALLES, Pamella Diogo; CASTRO, Fabio M.; KALIL, Jorge; YANG, Ariana Campos