ARIANA CAMPOS YANG
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina - Médico
LIM/60 - Laboratório de Imunologia Clínica e Alergia, Hospital das Clínicas, Faculdade de Medicina
LIM/60 - Laboratório de Imunologia Clínica e Alergia, Hospital das Clínicas, Faculdade de Medicina
7 resultados
Resultados de Busca
Agora exibindo 1 - 7 de 7
- Novel allergens from ancient foods: Man e 5 from manioc (Manihot esculenta Crantz) cross reacts with Hev b 5 from latex(2013) SANTOS, Keity Souza; GADERMAIER, Gabriele; VEJVAR, Eva; ARCURI, Helen Andrade; GALVAO, Clovis Eduardo; YANG, Ariana Campos; RESENDE, Virginia Maria Ferreira; MARTINS, Carlo de Oliveira; HIMLY, Martin; MARI, Adriano; LISO, Marina; POMPONI, Debora; BREITENEDER, Heimo; WAGNER, Stefan; KALIL, Jorge; FERREIRA, Fatima; CASTRO, Fabio Fernandes MoratoScope Manioc (Manihot esculenta) is a tuber mainly consumed in the Southern Hemisphere and used worldwide by food and chemistry industry. We aimed to recombinantly produce and characterize the first manioc allergen and evaluate its IgE reactivity in sera of Brazilian and Italian patients. Methods and results The molecule, termed Man e5, was expressed in E. coli, characterized by amino acid analysis, mass spectrometry, circular dichroism, HPLC, and dynamic light scattering. A tertiary structural model of the protein was produced using bioinformatics and susceptibility to pepsin digestion was analyzed in vitro. Based on its high content of charged residues, heat stability, flexibility and lack of secondary structure elements, the allergen was determined a member of the intrinsically disordered protein family. Brazilian patients were selected based on manioc allergy and Italians based on latex allergy and sensitization to Hev b 5.71% of Brazilians and 40% of Italians were in vitro IgE positive to Man e5. Cross-inhibition assays suggest a possible involvement of this allergen in the latex-fruit syndrome. Conclusion Man e5, the first purified allergen from manioc demonstrates IgE cross-reactivity with Hev b 5. Data suggest Hev b 5 might act as primary sensitizer and could therefore lead to allergic manifestations upon manioc consumption without prior exposition.
- Reactions to Shrimp Including Severe Anaphylaxis in Mite- and Cockroach-Allergic Patients Who Have Never Eaten Shrimp: Clinical Significance of IgE Cross-Reactivity to Tropomyosins From Different Sources(2019) MARTINS, T. F.; MENDONCA, T. N.; MELO, J. M. L.; MORENO, A. S.; JANUARIO, Y. C.; DASILVA, L. L. P.; DIAS, M. M.; MEIRELES, P. R.; SANTOS, K. S.; YANG, A. C.; ARRUDA, L. K.
- Allergic reactions to manioc (Manihot esculenta Crantz): Identification of novel allergens with potential involvement in latex-fruit syndrome(2011) SANTOS, Keity Souza; GALVAO, Clovis Eduardo; GADERMAIER, Gabriele; RESENDE, Virginia Maria Ferreira; MARTINS, Carlo de Oliveira; MISUMI, Denise Shimbo; YANG, Ariana Campos; FERREIRA, Fatima; PALMA, Mario Sergio; KALIL, Jorge; CASTRO, Fabio Fernandes Morato
conferenceObject Cross-Reactivity Among Cereal Grains(2016) MENDONCA, Juliana Guimaraes; CASTRO, Roberta Almeida; CORDOVA, Pablo Torres; MEIRELES, Paula Rezende; FIGO, Daniele Danella; SANTOS, Keity Souza; KALIL, Jorge; CASTRO, Fabio Fernandes Morato; YANG, Ariana C.conferenceObject EoE, OIT and Anaphylaxis: an unsolved puzzle.(2019) MARTINS, Catherine Sonaly Ferreira; TORRES, Pablo; ZANANDREA, Andressa; PENIDO, Antonio; MOUCO, Claudia Castilho; SILVA, Leticia Pereira da; ANAGUSKO, Claudia Leiko Yonekura; LOPES, Mariele Morandin; ASSIS, Joao Paulo de; MENDONCA, Juliana; SALLES-CUNHA, Patricia; SANTOS, Keity Souza; CASTRO, Fabio M.; YANG, Ariana C.conferenceObject Is IgA a piece of the EoE puzzle?(2020) MARTINS, C. S. F.; SUPRUN, M.; CUNHA, P. S.; SANTOS, K. S.; GUIMARAES, J. M.; CASTRO, F. F. M.; GRISHINA, G.; YANG, A. C.; SAMPSON, H. A.- Fructose biphosphate aldolase: A new cassava allergen(2023) VENTURA, Anne K. R. M.; ALVES, Safiri de P.; CASTRO, Roberta A.; ROSSINI, Bruno C.; DELAZARI, Lucilense S.; OLIVEIRA, Amanda M. de; MORETTI, Ana I. S.; CASTRO, Fabio F. M.; KALIL, Jorge; YANG, Ariana C.; SANTOS, Keity S.Background: Food allergy has considerably increased in recent years and this situation has been aggravated mainly by the consumption of more processed and complex foods, since minor or potentially allergenic foods are not required to be labeled. Manihot esculenta (cassava) is a widely consumed food in South America, Africa, and Asia and can be used in the production of flour and starch, as well as several other products. This root can cause allergic reactions with symptoms ranging from mild to severe. Methods: Thus, the aim of this study was the characterization of the immunogenic cassava proteins responsible for sensitizing patients allergic to it. Using a 2D-SDS-PAGE based proteomic approach, six proteins were identified, including Fructose Bisphosphate Aldolase (FBA). Recom-binant FBA was produced in Expi293 cells and evaluated by immunoblotting with the serum of 10 individual study subjects. Results: Our results showed six cassava IgE-reactive proteins. From those, recombinant fructose bisphosphate aldolase (FBA) showed a positivity of 80% among tested sera, proving to be a highly sensitizing protein. Conclusion: The recombinant FBA molecule obtained in this study can be important for in vivo diagnostic assays, by producing more accurate results, and for desensitization protocols, in which the use of the isolated molecule produces more precise results by avoiding secondary sensitization. Trial registration: All patients signed a consent form approved by the internal ethics committee CAPPesq, Comissao de etica para Analise de Projetos de Pesquisa do HC FMUSP (CAAE: 10420619.6.0000.0068).