ADOLFO WENJAW LIAO
Projetos de Pesquisa
Unidades Organizacionais
LIM/57 - Laboratório de Fisiologia Obstétrica, Hospital das Clínicas, Faculdade de Medicina
1 resultados
Resultados de Busca
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- Determination of Fetal RHD Genotype Including the RHD Pseudogene in Maternal Plasma(2017) ZIZA, Karen Chinoca; LIAO, Adolfo Wenjaw; DEZAN, Marcia; DINARDO, Carla Luana; JENS, Eduardo; FRANCISCO, Rossana Pulcineli Vieira; MENDRONE JUNIOR, Alfredo; ZUGAIB, Marcelo; LEVI, Jose EduardoObjective: To examine the accuracy of fetal RHD genotype and RHD pseudogene determination in a multiethnical population. Methods: Prospective study involving D-negative pregnant women. Cell-free DNA was extracted from 1 ml of maternal plasma by an automated system (MagNA Pure Compact, Roche) and real-time PCR was performed in triplicate targeting the RHD gene exons 5 and 7. Inconclusive samples underwent RHD pseudogene testing by real-time PCR analysis employing novel primers and probe. Results: A positive result was observed in 128/185 (69.2%) samples and negative in 50 (27.0%). Umbilical cord blood phenotype confirmed all cases with a positive or negative PCR result. Seven (3.8%) cases were found inconclusive (exon 7 amplification only) and RHD pseudogene testing with both conventional and real-time PCR demonstrated a positive result in five of them, while two samples were also RHD pseudogene negative. Conclusion: Real-time PCR targeting RHD exons 5 and 7 simultaneously in maternal plasma is an accurate method for the diagnosis of fetal D genotype in our population. The RHD pseudogene real-time PCR assay is feasible and is particularly useful in populations with a high prevalence of this allele. (C) 2016 Wiley Periodicals, Inc.