LEONARDO JENSEN SOCAS
Projetos de Pesquisa
Unidades Organizacionais
LIM/59 - Laboratório de Biologia Celular, Hospital das Clínicas, Faculdade de Medicina
8 resultados
Resultados de Busca
Agora exibindo 1 - 8 de 8
conferenceObject Methotrexate associated with a lipid core nanoparticle prevented the dilation and dissection of the aortic arch in mice with Marfan syndrome(2020) GUIDO, M. C.; LOPES, N. M.; I, C. Albuquerque; TAVARES, E. R.; JENSEN, L.; V, L. Pereira; KALIL-FILHO, R.; LAURINDO, F. R. M.; MARANHAO, R. C.- Pyridostigmine Bromide: Autonomic Nervous System Modulation Reduces Adipose and Splenic Tissue Weight in Leptin-Deficienty Ob/Ob Mice(2020) RIBEIRO, Amanda; SANTOS, Fernando dos; ARNOLD, Alexandre; BARBOSA, Maikon; JENSEN, Leonardo; IRIGOYEN, Maria Costa
conferenceObject Daunorubicin associated to lipid core nanoparticles reduces atherosclerotic lesions, inflammation and cardiotoxicity in atherosclerosis rabbit model(2020) I, C. Albuquerque; TAVARES, E. R.; GUIDO, M. G.; LOPES, N. M.; V, R. Baroni; JENSEN, L.; SILVA, B. M. O.; KALIL-FILHO, R.; TAVONI, T. M.; MARANHAO, R. C.- Wharton's jelly-derived mesenchymal stem cells attenuate sepsis-induced organ injury partially via cholinergic anti-inflammatory pathway activation(2020) CAPCHA, Jose Manuel Condor; RODRIGUES, Camila Eleuterio; MOREIRA, Roberto de Souza; SILVEIRA, Marcelo Duarte; DOURADO, Paulo; SANTOS, Fernando dos; IRIGOYEN, Maria Claudia; JENSEN, Leonardo; GARNICA, Margoth Ramos; NORONHA, Irene L.; ANDRADE, Lucia; GOMES, Samirah AbreuSepsis induces organ dysfunction due to overexpression of the inflammatory host response, resulting in cardiopulmonary and autonomic dysfunction, thus increasing the associated morbidity and mortality. Wharton's jellyderived mesenchymal stem cells (WJ-MSCs) express genes and secrete factors with anti-inflammatory properties, neurological and immunological protection, as well as improve survival in experimental sepsis. The cholinergic anti-inflammatory pathway (CAP) is mediated by alpha 7-nicotinic acetylcholine receptors (alpha 7nAChRs). which play an important role in the control of systemic inflammation. We hypothesized that WJ-MSCs attenuate sepsis-induced organ injury in the presence of an activated CAP pathway. To confirm our hypothesis, we evaluated the effects of WJ-MSCs as a treatment for cardiopulmonary injury and on neuroimmunomodulation. Male Wistar rats were randomly divided into four groups: control (sham-operated); cecal ligation and puncture (CLP) alone; CL.P+WJ-MSCs (1 x 10(6) cells, at 6 h post-CLP); and CLP+methyllycaconifine (MLA)+WJ-MSCs (5 mg/kg body wt, at 53 h post-CLP, and 1 x 10(6) cells, at 6 h post-CLP. respectively). All experiments, including the assessment of echocardiographic parameters and heart rate variability, were performed 24 h after CLP. WJ-MSC treatment attenuated diastolic dysfunction and restored baroreflex sensitivity. WJ-MSCs also increased cardiac sympathetic and cardiovagal activity. WJ-MSCs reduced leukocyte infiltration and proinflammatory cytokines, effects that were abolished by administration of a selective alpha 7nAChR antagonist (MLA). In addition, WJ-MSC treatment also diminished apoptosis in the lungs and spleen. In cardiac and splenic tissue, WJ-MSCs downregulated alpha 7nAChR expression, as well as reduced the phospho-STAT3-tototal STAT3 ratio in the spleen. WJ-MSCs appear to protect against sepsis-induced organ injury by reducing systemic inflammation, at least in part, via a mechanism that is dependent on an activated CAP.
conferenceObject Methotrexate Carried in Lipid Core Nanoparticles Prevented the Dilation and Dissection of the Aortic Arch in Mice With Marfan Syndrome by Increasing the Bioavailability of Intracellular Adenosine(2020) GUIDO, Maria Carolina; LOPES, Natalia M.; ALBUQUERQUE, Camila I.; TAVARES, Elaine R.; JENSEN, Leonardo; PEREIRA, Lygia V.; LAURINDO, Francisco R.; MARANHAO, Raul C.conferenceObject Left Ventricle Dysfunction is Prevented by the Treatment With Methotrexate Carried in Lipid Nanoparticles in Lipopolysaccharide-injected Rats(2020) LOPES, Natalia M.; GUIDO, Maria Carolina; ALBUQUERQUE, Camila; JENSEN, Leonardo; MARANHAO, Raul C.- Postprandial increase in glucagon-like peptide-1 is blunted in severe heart failure(2020) ARRUDA-JUNIOR, Daniel F.; MARTINS, Flavia L.; SALLES, Thiago Almeida; JENSEN, Leonardo; DARIOLLI, Rafael; ANTONIO, Ednei L.; SANTOS, Leonardo dos; CRAJOINAS, Renato O.; TUCCI, Paulo J. F.; GOWDAK, Luis Henrique W.; KRIEGER, Jose Eduardo; PEREIRA, Alexandre C.; GIRARDI, Adriana C.The relationship between disturbances in glucose homeostasis and heart failure (HF) progression is bidirectional. However, the mechanisms by which HF intrinsically impairs glucose homeostasis remain unknown. The present study tested the hypothesis that the bioavailability of intact glucagon-like peptide-1 (GLP-1) is affected in HF, possibly contributing to disturbed glucose homeostasis. Serum concentrations of total and intact GLP-1 and insulin were measured after an overnight fast and 15 min after the ingestion of a mixed breakfast meal in 49 non-diabetic patients with severe HF and 40 healthy control subjects. Similarly, fasting and postprandial serum concentrations of these hormones were determined in sham-operated rats, and rats with HF treated with an inhibitor of the GLP-1-degrading enzyme dipeptidyl peptidase-4 (DPP4), vildagliptin, or vehicle for 4 weeks. We found that HF patients displayed a much lower increase in postprandial intact and total GLP-1 levels than controls. The increase in postprandial intact GLP-1 in HF patients correlated negatively with serum brain natriuretic peptide levels and DPP4 activity and positively with the glomerular filtration rate. Likewise, the postprandial increases in both intact and total GLP-1 were blunted in HF rats and were restored by DPP4 inhibition. Additionally, vehicle-treated HF rats displayed glucose intolerance and hyperinsulinemia, whereas normal glucose homeostasis was observed in vildagliptin-treated HF rats. We conclude that the postprandial increase in GLP-1 is blunted in non-diabetic HF. Impaired GLP-1 bioavailability after meal intake correlates with poor prognostic factors and may contribute to the establishment of a vicious cycle between glucose disturbance and HF development and progression.
conferenceObject Treatment with methotrexate carried in lipid core nanoparticles prevents the development of diastolic dysfunction in septic rats(2020) LOPES, N. M.; GUIDO, M. C.; ALBUQUERQUE, C. L.; JENSEN, L.; MIURA, V. M.; MARINHO, L. M.; MARANHAO, R. C.