VERA DEMARCHI AIELLO
Projetos de Pesquisa
Unidades Organizacionais
Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina
7 resultados
Resultados de Busca
Agora exibindo 1 - 7 de 7
- A ecocardiografia no laboratorio cardiovascular: um guia para a pesquisa com animais(2014) ABDUCH, Maria Cristina Donadio; ASSAD, Renato Samy; MATHIAS JR, Wilson; AIELLO, Vera DemarchiThe feasibility and potential for the morphological and hemodynamic investigation of the heart has been increasing the use of the echocardiography in the research setting. Additionally, the development of new technologies, like the real time 3D echocardiography and speckle tracking, demands validation throughout experimental studies before being instituted in the clinical setting. This paper aims to provide information concerning the particularities of the echocardiographic examination in quadruped mammals, targeting the experimental research.
conferenceObject Reversible Pulmonary Trunk Banding. XI: Myocardial Vascular Endothelial Growth Factor Expression of Young Goats Submitted to Rapid Ventricle Retraining(2016) ASSAD, Renato S.; ROCHA, Eduardo A.; MENICONI, Tiago A.; ABDUCH, Maria Cristina D.; MOREIRA, Luiz Felipe P.; AIELLO, Vera D.; JATENE, Fabio B.- Reversible pulmonary trunk banding: Myocardial vascular endothelial growth factor expression in young goats submitted to ventricular retraining(2020) ASSAD, Renato S.; V, Eduardo A. Rocha; AIELLO, Vera D.; MENICONI, Tiago A.; ABDUCH, Maria C. D.; THOMAZ, Petronio G.; JATENE, Marcelo B.; MOREIRA, Luiz F. P.Background Ventricle retraining has been extensively studied by our laboratory. Previous studies have demonstrated that intermittent overload causes a more efficient ventricular hypertrophy. The adaptive mechanisms involved in the ventricle retraining are not completely established. This study assessed vascular endothelial growth factor (VEGF) expression in the ventricles of goats submitted to systolic overload. Methods Twenty-one young goats were divided into 3 groups (7 animals each): control, 96-hour continuous systolic overload, and intermittent systolic overload (four 12-hour periods of systolic overload paired with 12-hour resting period). During the 96-hour protocol, systolic overload was adjusted to achieve a right ventricular (RV) / aortic pressure ratio of 0.7. Hemodynamic evaluations were performed daily before and after systolic overload. Echocardiograms were obtained preoperatively and at protocol end to measure cardiac masses thickness. At study end, the animals were killed for morphologic evaluation and immunohistochemical assessment of VEGF expression. Results RV-trained groups developed hypertrophy of RV and septal masses, confirmed by increased weight and thickness, as expected. In the study groups, there was a small but significantly increased water content of the RV and septum compared with those in the control group (p<0.002). VEGF expression in the RV myocardium was greater in the intermittent group (2.89% +/- 0.41%) than in the continuous (1.80% +/- 0.19%) and control (1.43% +/- 0.18%) groups (p<0.023). Conclusions Intermittent systolic overload promotes greater upregulation of VEGF expression in the sub-pulmonary ventricle, an adaptation that provides a mechanism for increased myocardial perfusion during the rapid myocardial hypertrophy of young goats.
- When the description of physiology adds to the morphological diagnosis and guides the analysis of clinical and surgical management(2022) AIELLO, Vera Demarchi; ASSAD, Renato Samy
conferenceObject Remote Ischemic Preconditioning Does Not Prevent Placental Dysfunction Induced by Extrcorporeal Circulation in Fetal Lambs(2019) ASSAD, Renato S.; GUEDES, Marcelo G.; AIELLO, Vera D.; THOMAZ, Petronio G.; ZANONI, Fernando L.; ARASHIRO, Ana Cristina A.; SILVA, Ana Paula N. da; SILVA, Raphael S. Coutinho e; FALOPPA, Ana Cristina B.; PINTO, Marcelo V.; JATENE, Marcelo B.; MOREIRA, Luiz Felipe P.- Reversible Pulmonary Trunk Banding VIII: Intermittent Overload Causes Harmless Hypertrophy in Adult Goat(2013) MIANA, Leonardo A.; ASSAD, Renato S.; ABDUCH, Maria C. D.; SILVA, Gustavo J. J.; NOGUEIRA, Ananda R.; AIELLO, Vera D.; MOREIRA, Luiz Felipe P.Background. Traditional pulmonary artery banding (PAB) is not always suitable for mature subpulmonary ventricle retraining. We sought to assess in detail the myocardial morphologic adaptations of two different protocols for inducing right ventricular (RV) hypertrophy in an adult animal model. Methods. Eighteen adult goats were distributed into three groups: sham (no systolic overload), traditional (continuous systolic overload), and intermittent (daily 12-hour systolic overload). Systolic overload was adjusted to achieve a 0.7 RV-to-aortic pressure ratio. All animals underwent weekly echocardiographic studies, and hemodynamic evaluations were performed 3 times a week. After 4 weeks, the animals were humanely killed for morphologic assessment. Results. A 37.2% increase was observed in the RV wall thickness of the intermittent group (p < 0.05), but no significant echocardiographic changes were observed in the other two groups. The intermittent and traditional groups had a 55.7% and 36.7% increase in RV mass, respectively, compared with the sham group (p < 0.05). No differences were observed in myocardial water content of the three groups (p = 0.27). RV myocardial fiber and nuclei diameters were increased in the intermittent group compared with the sham group (p < 0.05). The area of collagen deposition in the RV interstitium was increased 98% in traditional group compared with the sham group (p < 0.05). No significant cellular proliferation occurred in any group. Conclusions. This study suggests that a more effective and harmless hypertrophy can be achieved in adult animals using intermittent PAB compared with the traditional approach. (Ann Thorac Surg 2013;95:1422-8) (c) 2013 by The Society of Thoracic Surgeons
- Ischemic preconditioning does not prevent placental dysfunction induced by fetal cardiac bypass(2022) ASSAD, Renato S.; GUEDES, Marcelo G. A.; AIELLO, Vera D.; THOMAZ, Petronio G.; ZANONI, Fernando L.; SAITO, Mauricio; SILVA, Ana Paula N. da; SILVA, Raphael dos S. Coutinho e; V, Marcelo Pinto; JATENE, Marcelo B.; MOREIRA, Luiz Felipe P.Background Remote ischemic preconditioning (rIPC) has been applied to attenuate tissue injury. We tested the hypothesis that rIPC applied to fetal lambs undergoing cardiac bypass (CB) reduces fetal systemic inflammation and placental dysfunction. Methods Eighteen fetal lambs were divided into three groups: sham, CB control, and CB rIPC. CB rIPC fetuses had a hindlimb tourniquet applied to occlude blood flow for four cycles of a 5-min period, followed by a 2-min reperfusion period. Both study groups underwent 30 min of normothermic CB. Fetal inflammatory markers, gas exchange, and placental and fetal lung morphological changes were assessed. Results The CB rIPC group achieved higher bypass flow rates (p < .001). After CB start, both study groups developed significant decreases in PaO2, mixed acidosis, and increased lactate levels (p < .0004). No significant differences in tissular edema were observed on fetal lungs and placenta (p > .391). Expression of Toll-like receptor 4 and intercellular adhesion molecule-1 in the placenta and fetal lungs did not differ among the three groups, as well as with vascular cell adhesion molecule-1 (VCAM-1) of fetal lungs (p > .225). Placental VCAM-1 expression was lower in the rIPC group (p < .05). Fetal interleukin-1 (IL-1) and thromboxane A2 (TXA2) levels were lower at 60 min post-CB in the CB rIPC group (p < .05). There were no significant differences in tumor necrosis factor-alpha, prostaglandin E2, IL-6, and IL-10 plasma levels of the three groups at 60-min post-bypass (p > .133). Conclusion Although rIPC allowed increased blood flow during fetal CB and decreased IL-1 and TXA2 levels and placental VCAM-1, it did not prevent placental dysfunction in fetal lambs undergoing CB.