LUDMILA RODRIGUES PINTO FERREIRA CAMARGO
Projetos de Pesquisa
Unidades Organizacionais
LIM/19 - Laboratório de Histocompatibilidade e Imunidade Celular, Hospital das Clínicas, Faculdade de Medicina
9 resultados
Resultados de Busca
Agora exibindo 1 - 9 de 9
- The Combined Deficiency of Immunoproteasome Subunits Affects Both the Magnitude and Quality of Pathogen- and Genetic Vaccination-Induced CD8(+) T Cell Responses to the Human Protozoan Parasite Trypanosoma cruzi(2016) ERSCHING, Jonatan; VASCONCELOS, Jose R.; FERREIRA, Camila P.; CAETANO, Braulia C.; MACHADO, Alexandre V.; BRUNA-ROMERO, Oscar; BARON, Monique A.; FERREIRA, Ludmila R. P.; CUNHA-NETO, Edecio; ROCK, Kenneth L.; GAZZINELLI, Ricardo T.; RODRIGUES, Maurcio M.The beta 1i, beta 2i and beta 5i immunoproteasome subunits have an important role in defining the repertoire of MHC class I-restricted epitopes. However, the impact of combined deficiency of the three immunoproteasome subunits in the development of protective immunity to intracellular pathogens has not been investigated. Here, we demonstrate that immunoproteasomes play a key role in host resistance and genetic vaccination-induced protection against the human pathogen Trypanosoma cruzi (the causative agent of Chagas disease), immunity to which is dependent on CD8(+) T cells and IFN-gamma (the classical immunoproteasome inducer). We observed that infection with T. cruzi triggers the transcription of immunoproteasome genes, both in mice and humans. Importantly, genetically vaccinated or T. cruziinfected beta 1i, beta 2i and beta 5i triple knockout (TKO) mice presented significantly lower frequencies and numbers of splenic CD8(+) effector T cells (CD8(+) CD44(high)CD62L(low)) specific for the previously characterized immunodominant (VNHRFTLV) H-2K(b)-restricted T. cruzi epitope. Not only the quantity, but also the quality of parasite-specific CD8(+) T cell responses was altered in TKO mice. Hence, the frequency of double-positive (IFN-gamma(+)/TNF+) or single-positive (IFN-gamma(+)) cells specific for the H-2K(b)-restricted immunodominant as well as subdominant T. cruzi epitopes were higher inWT mice, whereas TNF single-positive cells prevailed among CD8(+) T cells from TKO mice. Contrasting with their WT counterparts, TKO animals were also lethally susceptible to T. cruzi challenge, even after an otherwise protective vaccination with DNA and adenoviral vectors. We conclude that the immunoproteasome subunits are key determinants in host resistance to T. cruzi infection by influencing both the magnitude and quality of CD8(+) T cell responses.
conferenceObject Gene Expression Profile in Patients with Classical Hodgkin Lymphoma in Brazil: Results from a Pilot Study(2016) SILVA, Priscilla Brito; REAL, Juliana Monte; FERREIRA, Ludmila Rodrigues Pinto; ESTEVES, Gustavo Henrique; GARIBALDI JUNIOR, Joao; PENNA, Adriana M. Damasco; BRITO, Fabio Nascimento; CAVALCANTE, Egyla; BAIOCCHI, Otavio C. G.conferenceObject Impact of Treatment on IL-10, CTLA-4 and PD-L1 Gene Expression in Patients with Classical Hodgkin Lymphoma(2016) SILVA, Priscilla Brito; REAL, Juliana Monte; FERREIRA, Ludmila Rodrigues Pinto; ESTEVES, Gustavo Henrique; GARIBALDI JUNIOR, Joao; PENNA, Adriana M. Damasco; BRITO, Fabio Nascimento; CAVALCANTE, Egyla; BAIOCCHI, Otavio C. G.conferenceObject Increased Circulating Levels of miR-204 miR-101 and miR-425 in Recent Onset Type 1 Diabetes Can Be a Marker of Beta-Cell Destruction and Dysregulation of Insulin Signaling(2016) SANTOS, Aritania S.; FERREIRA, Ludmila R. P.; FUKUI, Rosa T.; CUNHA-NETO, Edecio; SILVA, Maria Elizabeth R.- Myocardial Infarction-Associated Transcript, a Long Noncoding RNA, Is Overexpressed During Dilated Cardiomyopathy Due to Chronic Chagas Disease(2016) FRADE, Amanda Farage; LAUGIER, Laurie; FERREIRA, Ludmila Rodrigues Pinto; BARON, Monique Andrade; BENVENUTI, Luiz Alberto; TEIXEIRA, Priscila Camillo; NAVARRO, Isabela Cunha; CABANTOUS, Sandrine; FERREIRA, Frederico Moraes; CANDIDO, Darlan da Silva; GAIOTTO, Fabio Antonio; BACAL, Fernando; POMERANTZEFF, Pablo; SANTOS, Ronaldo Honorato Barros; KALIL, Jorge; CUNHA-NETO, Edecio; CHEVILLARD, ChristopheLong noncoding RNAs (lncRNAs) modulate gene expression at the epigenetic, transcriptional, and posttranscriptional levels. Dysregulation of the lncRNA known as myocardial infarction-associated transcript (MIAT) has been associated with myocardial infarction. Chagas disease causes a severe inflammatory dilated chronic cardiomyopathy (CCC). We investigated the role of MIAT in CCC. A whole-transcriptome analysis of heart biopsy specimens and formalin-fixed, paraffin-embedded samples revealed that MIAT was overexpressed in patients with CCC, compared with subjects with noninflammatory dilated cardiomyopathy and controls. These results were confirmed in a mouse model. Results suggest that MIAT is a specific biomarker of CCC.
conferenceObject IMPACT OF TREATMENT ON RNA EXPRESSION OF IMMUNE PROFILE IN PATIENTS WITH CLASSICAL HODGKIN LYMPHOMA(2016) SILVA, P.; REAL, J. Monte; FERREIRA, L.; ESTEVES, G.; GARIBALDI JR., J.; BRITO, F.; PENNA, A.; CAVALCANTE, E.; BAIOCCHI, O.conferenceObject Epstein-Barr Virus Related Classical Hodgkin Lymphoma Reveals a Pro-Inflammatory RNA Expression Profile at Diagnosis(2016) SILVA, Priscilla Brito; REAL, Juliana Monte; FERREIRA, Ludmila Rodrigues Pinto; PENNA, Adriana M. Damasco; ESTEVES, Gustavo Henrique; GARIBALDI JUNIOR, Joao; BRITO, Fabio Nascimento; CAVALCANTE, Egyla; BAIOCCHI, Otavio C. G.conferenceObject Gene Networks and Canonical Pathways Analysis in Classical Hodgkin Lymphoma Patients(2016) SILVA, Priscilla Brito; REAL, Juliana Monte; BAIOCCHI, Otavio C. G.; ESTEVES, Gustavo Henrique; GARIBALDI JUNIOR, Joao; BRITO, Fabio Nascimento; PENNA, Adriana M. Damasco; CAVALCANTE, Egyla; FERREIRA, Ludmila Rodrigues PintoconferenceObject System biology approaches to identify Gene-microRNA pathophysiology related networks in Chagas disease(2016) FERREIRA, L. R. P.; LAUGIER, L.; FERREIRA, F. M.; CABANTOUS, S.; CANDIDO, D. D. S.; ROZANSKI, A.; LANNES-VIEIRA, J.; CHEVILLARD, C.; KALIL, J.; CUNHA-NETO, E.