JOEL CLAUDIO HEIMANN
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Clínica Médica, Faculdade de Medicina - Docente
LIM/16 - Laboratório de Fisiopatologia Renal, Hospital das Clínicas, Faculdade de Medicina - Líder
LIM/16 - Laboratório de Fisiopatologia Renal, Hospital das Clínicas, Faculdade de Medicina - Líder
17 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 17
conferenceObject Myocardial AT1 gene expression is increased in a model of left ventricular hypertrophy induced by high salt intake(2013) PEREIRA, Rafael Canavel; KATAYAMA, Isis Akemi; HEIMANN, Joel ClaudioObjective: To evaluate gene expression of the renin-angiotensin system components in a model of left ventricular hypertrophy induced by high salt intake demonstrated in a previous study. Methods: Wistar rats were fed normal (NS1.3% NaCl) or high (HS 8%) salt diet since weaning. From the 7th week of age, two HS subgroups received hydralazine or losartan (HZ 15 and LOS 20mg/kg/day). Angiotensinogen, renin, ACE, ACE2, AT1 and AT2 gene expression in left ventricle were measured. Data are reported as mean±SEM. Means in row with superscripts without a common letter differ, P<0.05. Conclusion: High salt intake increases AT1 gene expression, however it is prevented by losartan treatment.conferenceObject Effect of an angiotensin II type 2 receptor agonist on myocardial hypertrophy and fibrosis induced by high salt diet(2017) DOPONA, Ellen Priscila Brito; ROCHA, Veronica Favoni; OLIVEIRA, Ivone Braga de; FURUKAWA, Luzia Naoko Shinohara; HEIMANN, Joel ClaudioconferenceObject Small litter size at birth is associated with insulin resistance and low energy expenditure in adulthood(2013) TAVARES, Neuziane Kloos Amorim; AGUIAR, Fernanda de Jesus; FURUKAWA, Luzia Naoko; HEIMANN, Joel ClaudioObjective: To evaluate the effect of litter size standardization at birth on adult metabolic parameters.Methods: Female Wistar rats became pregnant at 12 weeks of age. Offspring were divided into control(C), G8(8) and G4(4 newborns/litter) groups. At 12 weeks of age, food intake (FI,g/day), glucose (G,mg/dL), insulin (I,pM), cholesterol (Chol,mg/dL), triacylglycerols (TAG,mg/dL) levels, white and brown adipose tissue (BAT) masses (g/100g) adiposity index (AI,% body weight) were determined. Conclusion: Results suggest that changes in litter size is associated with energy balance, carbohydrate and lipid disorders, insulin resistance and alterations in blood pressure in adult male and female.conferenceObject Mitochondria function and structure in brown adipose tissue is influenced by maternal low salt intake during the perinatal period(2012) LOPES, Karen Lucasechi; SANTOS, Elisabete Alcantara Dos; FURUKAWA, Luzia Naoko Shinohara; HEIMANN, Joel Claudio- Isolated total RNA and protein are preserved after thawing for more than twenty-four hours(2012) OLIVEIRA, Ivone Braga de; RAMOS, Debora Rothstein; LOPES, Karen Lucasechi; SOUZA, Regiane Machado de; HEIMANN, Joel Claudio; FURUKAWA, Luzia Naoko ShinoharaOBJECTIVE: The preservation of biological samples at a low temperature is important for later biochemical and/or histological analyses. However, the molecular viability of thawed samples has not been studied sufficiently in depth. The present study was undertaken to evaluate the viability of intact tissues, tissue homogenates, and isolated total RNA after defrosting for more than twenty-four hours. METHODS: The molecular viability of the thawed samples (n = 82) was assessed using the A260/A280 ratio, the RNA concentration, the RNA integrity, the level of intact mRNA determined by reverse transcriptase polymerase chain reaction, the protein level determined by Western blotting, and an examination of the histological structure. RESULTS: The integrity of the total RNA was not preserved in the thawed intact tissue, but the RNA integrity and level of mRNA were perfectly preserved in isolated defrosted samples of total RNA. Additionally, the level of beta-actin protein was preserved in both thawed intact tissue and homogenates. CONCLUSION: Isolated total RNA does not undergo degradation due to thawing for at least 24 hours, and it is recommended to isolate the total RNA as soon as possible after tissue collection. Moreover, the protein level is preserved in defrosted tissues.
conferenceObject Lysine Specific Demethylase-1 Deficiency Accelerates the Development of Hypertension and Renal Damage During Long Term Sodium Intake(2019) KATAYAMA, Isis Akemi; CULLINANE, Danielle L.; WILLIAMS, Gordon H.; HEIMANN, Joel Claudio; POJOGA, Luminita H.- The effects of individually ventilated cages on the respiratory systems of male and female Wistar rats from birth until adulthood(2017) MARCHESI, Guilherme DAprile; SOTO, Sonia de Fatima; CASTRO, Isac de; RODRIGUES, Thiago Guimaraes; MORIYA, Henrique Takachi; ALMEIDA, Francine Maria de; PAZETTI, Rogerio; HEIMANN, Joel Claudio; FURUKAWA, Luzia Naoko ShinoharaOBJECTIVE: To evaluate the respiratory systems of male and female rats maintained in individually ventilated cages (IVCs) from birth until adulthood. METHODS : Female Wistar rats were housed in individually ventilated cages or conventional cages (CCs) and mated with male Wistar rats. After birth and weaning, the male offspring were separated from the females and kept in cages of the same type until 12 weeks of age. RESULTS: The level of food consumption was lower in male offspring (IVC=171.7 +/- 9; CC=193.1 +/- 20) than in female offspring (IVC=100.6 +/- 7; CC=123.4 +/- 0.4), whereas the water intake was higher in female offspring (IVC=149.8 +/- 11; CC=99.2 +/- 0) than in male offspring (IVC=302.5 +/- 25; CC=249.7 +/- 22) at 11 weeks of age when housed in IVCs. The cage temperature was higher in individually ventilated cages than in conventional cages for both male (IVCs=25.9 +/- 0.5; CCs=22.95 +/- 0.3) and female (IVCs=26.2 +/- 0.3; CCs=23.1 +/- 0.3) offspring. The respiratory resistance (IVC=68.8 +/- 2.8; CC=50.6 +/- 3.0) and elastance (IVC=42.0 +/- 3.9; CC=32.4 +/- 2.0) at 300 mm/kg were higher in the female offspring housed in ventilated cages. The ciliary beat values were lower in both the male (IVCs=13.4 +/- 0.2; CC=15 +/- 0.4) and female (IVC=13.5 +/- 0.4; CC=15.9 +/- 0.6) offspring housed in individually ventilated cages than in those housed in conventional cages. The total cell (IVC=117.5 +/- 9.7; CC=285.0 +/- 22.8), neutrophil (IVC=13.1 +/- 4.8; CC=75.6 +/- 4.1) and macrophage (IVC=95.2 +/- 11.8; CC=170.0 +/- 18.8) counts in the bronchoalveolar lavage fluid were lower in the female offspring housed in individually ventilated cages than in those housed in conventional cages. CONCLUSIONS : The environmental conditions that exist in individually ventilated cages should be considered when interpreting the results of studies involving laboratory animals. In this study, we observed gender dimorphism in both the water consumption and respiratory mechanics of rats kept in ventilated cages.
conferenceObject Male Mice Heterozygous LSD1 Knockout Gene Prevented the Effect of High Sodium Intake on Renal Fibrosis and AT1 Conformational Status(2019) NASCIMENTO, Mariana Moura; KATAYAMA, Isis Akemi; POJOGA, Luminita H.; HEIMANN, Joel Claudio- Inhalation of fine particulate matter during pregnancy increased IL-4 cytokine levels in the fetal portion of the placenta(2015) MELO, Juliana Oliveira de; SOTO, Sonia Fatima; KATAYAMA, Isis Akemi; WENCESLAU, Camilla Ferreira; PIRES, Amanda Gonalves; VERAS, Mariana Matera; FURUKAWA, Luzia N. S.; CASTRO, Isac de; SALDIVA, Paulo Hilario Nascimento; HEIMANN, Joel ClaudioThis study aimed to verify the development of placental and systemic inflammation in rats exposed to fine particulate matter before or during pregnancy. Wistar rats were exposed to filtered air ( control) or to a load of 600 mu g/m(3) of fine particles in the air. The gene expression of IL-1 beta, IL-4, IL-6, IL-10, INF-gamma, TNF-alpha and Toll-like receptor 4 in the placenta was evaluated. The serum and placental concentrations of IL-1 beta, IL-4, IL-6, IL-10, INF-gamma and TNF-alpha were measured. The total and differential blood leukocyte and blood platelet count was assessed. Compared to control animals, IL-4 content was elevated in the fetal portion of the placenta in rats exposed to air pollution before and during pregnancy. Increased IL-4 suggests that a placental inflammatory reaction may have occurred in response to exposure to fine particulate matter and that this cytokine was responsible, among possibly others factors, for resolution of the inflammatory reaction.
conferenceObject Glucose metabolism and DNA methylation of offspring are altered by maternal sodium restriction(2013) SIQUEIRA, Flavia Ramos; PhuongSon Nguyen; KOBZIK, Lester; OLIVEIRA, Ivone Braga de; HEIMANN, Joel Claudio; FURUKAWA, Luzia Naoko ShinoharaObjective: To evaluate glucose metabolism of adult offspring of dams fed low sodium diet during pregnancy. Methods: Female Wistar rats (n= 6 – 8/group) were fed low (LS- 0.15%) or normal (NS-1.3% NaCl) salt diet since the 1st day of gestation until delivery or LS during 1st (LS10) or 2 nd half of gestation (LS20). Body weight (BW), circulating glucose, insulin, leptin and C-peptide levels, glucose (GTT) and insulin tolerance test (ITT) and leptin receptor (LR) methylation were measured in male (M) and female (F) offspring at 12 weeks of age Results (mean±SEM, p< 0.05): BW, at birth (g, n=14 – 27/group) (M: NS = 6.5±0.1, LS = 5.5±0.1, LS10 = 6±0.1, LS20 = 4.8±0.1, F: NS = 6±0.2, LS = 5±0.1, LS10 = 6±0.1, LS20 = 4±0.1) was lower in LS, LS10 and LS20 than in NS, while at adulthood it was not different. Glucose was higher in LS (M=115±3; F=110±4) than in the other groups (M: NS=103±1; LS10=100±2; LS20=96±2; F:NS=101±2; LS10=100±2; 108±2). C-peptide was higher in LS (M=1117±152; F=509±27) than in NS (M=375±41; F=233±63) and lower in LS20 (M=97±15; F=76±9) compared to LS10 (M=528±112; F=378±45).Glucose was higher in LS M and F at 5 minutes of the GTT compared to NS offspring. Leptin, insulin, and ITT were not different. But LR methylation was increased in LS20. Conclusion: Sodium restriction during pregnancy leads to low birth weight and to modifications of glucose metabolism and LR methylation.