JOEL CLAUDIO HEIMANN
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Clínica Médica, Faculdade de Medicina - Docente
LIM/16 - Laboratório de Fisiopatologia Renal, Hospital das Clínicas, Faculdade de Medicina - Líder
LIM/16 - Laboratório de Fisiopatologia Renal, Hospital das Clínicas, Faculdade de Medicina - Líder
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conferenceObject Myocardial AT1 gene expression is increased in a model of left ventricular hypertrophy induced by high salt intake(2013) PEREIRA, Rafael Canavel; KATAYAMA, Isis Akemi; HEIMANN, Joel ClaudioObjective: To evaluate gene expression of the renin-angiotensin system components in a model of left ventricular hypertrophy induced by high salt intake demonstrated in a previous study. Methods: Wistar rats were fed normal (NS1.3% NaCl) or high (HS 8%) salt diet since weaning. From the 7th week of age, two HS subgroups received hydralazine or losartan (HZ 15 and LOS 20mg/kg/day). Angiotensinogen, renin, ACE, ACE2, AT1 and AT2 gene expression in left ventricle were measured. Data are reported as mean±SEM. Means in row with superscripts without a common letter differ, P<0.05. Conclusion: High salt intake increases AT1 gene expression, however it is prevented by losartan treatment.conferenceObject Small litter size at birth is associated with insulin resistance and low energy expenditure in adulthood(2013) TAVARES, Neuziane Kloos Amorim; AGUIAR, Fernanda de Jesus; FURUKAWA, Luzia Naoko; HEIMANN, Joel ClaudioObjective: To evaluate the effect of litter size standardization at birth on adult metabolic parameters.Methods: Female Wistar rats became pregnant at 12 weeks of age. Offspring were divided into control(C), G8(8) and G4(4 newborns/litter) groups. At 12 weeks of age, food intake (FI,g/day), glucose (G,mg/dL), insulin (I,pM), cholesterol (Chol,mg/dL), triacylglycerols (TAG,mg/dL) levels, white and brown adipose tissue (BAT) masses (g/100g) adiposity index (AI,% body weight) were determined. Conclusion: Results suggest that changes in litter size is associated with energy balance, carbohydrate and lipid disorders, insulin resistance and alterations in blood pressure in adult male and female.conferenceObject Small litter size at birth is associated with insulin resistance and low energy expenditure in adulthood(2013) TAVARES, N. K. A.; AGUIAR, F. J.; FURUKAWA, L. N. S.; HEIMANN, J. C.Objective: To evaluate the effect of litter size standardization at birth on adult metabolic parameters. Methods: Female Wistar rats were mated and became pregnant at 12 weeks of age. Offspring were divided into control(C), S8(8) and S4(4 newborns/litter) groups. At 12 weeks of age, food intake (FI,g/day), glucose (G,mg/dL), insulin (I,pM), cholesterol (Chol,mg/dL), triacylglycerols (TAG,mg/dL) levels, white and brown adipose tissue (BAT) masses (g/100g) adiposity index (AI,% BW) were determined. Results: (mean±SEM p<0.05, n=6to32)1p<0.05 vs C and S8; 2p<0.05 vs S8 and S4; 3p<0.05 vs C; 4p<0.05 vs S8 Conclusion: Results suggest that the litter size is associated with insulin resistance and low energy expenditure in adulthood.- Salt intake during pregnancy alters offspring's myocardial structure(2013) ALVES-RODRIGUES, E. N.; VERAS, M. M.; ROSA, K. T.; CASTRO, I. de; FURUKAWA, L. N. S.; OLIVEIRA, I. B.; SOUZA, R. M.; HEIMANN, J. C.Background and Aim: To evaluate the effects of low or high salt intake during pregnancy on left ventricle of adult male offspring. Methods and results: Low-(LS, 0.15%), normal-(NS, 1.3%) or high-salt (HS, 8% NaCl) diet was given to Wistar rats during pregnancy. During lactation all dams received NS as well as the offspring after weaning. To evaluate cardiac response to salt overload, 50% of each offspring group was fed a high-salt (hs, 4% NaCl) diet from the 21st to the 36th week of age (LShs, NShs, HShs). The remaining 50% was maintained on NS (LSns, NSns and HSns). Echocardiography was done at 20 and 30 weeks of age. Mean blood pressure (MBP), histology and left ventricular angiotensin II content (AII) were analyzed at 36 weeks of age. Interventricular septum, left ventricular posterior wall and relative wall thickness increased from the 20th to the 30th week of age only in HShs, cardiomyocyte mean volume was higher in HShs compared to NShs, LShs and HSns. AII and left ventricular fibrosis were not different among groups. Conclusions: HS during pregnancy programs adult male offspring to a blood pressure and angiotensin II independent concentric left ventricular hypertrophy, with no fibrosis, in response to a chronic high-salt intake.
conferenceObject Effects of exposure do air pollution on placental structure(2013) SOTO, Sonia de Fatima; LOPES, Karen; VERAS, Mariana Matera; HEIMANN, JoelconferenceObject Glucose metabolism and DNA methylation of offspring are altered by maternal sodium restriction(2013) SIQUEIRA, Flavia Ramos; PhuongSon Nguyen; KOBZIK, Lester; OLIVEIRA, Ivone Braga de; HEIMANN, Joel Claudio; FURUKAWA, Luzia Naoko ShinoharaObjective: To evaluate glucose metabolism of adult offspring of dams fed low sodium diet during pregnancy. Methods: Female Wistar rats (n= 6 – 8/group) were fed low (LS- 0.15%) or normal (NS-1.3% NaCl) salt diet since the 1st day of gestation until delivery or LS during 1st (LS10) or 2 nd half of gestation (LS20). Body weight (BW), circulating glucose, insulin, leptin and C-peptide levels, glucose (GTT) and insulin tolerance test (ITT) and leptin receptor (LR) methylation were measured in male (M) and female (F) offspring at 12 weeks of age Results (mean±SEM, p< 0.05): BW, at birth (g, n=14 – 27/group) (M: NS = 6.5±0.1, LS = 5.5±0.1, LS10 = 6±0.1, LS20 = 4.8±0.1, F: NS = 6±0.2, LS = 5±0.1, LS10 = 6±0.1, LS20 = 4±0.1) was lower in LS, LS10 and LS20 than in NS, while at adulthood it was not different. Glucose was higher in LS (M=115±3; F=110±4) than in the other groups (M: NS=103±1; LS10=100±2; LS20=96±2; F:NS=101±2; LS10=100±2; 108±2). C-peptide was higher in LS (M=1117±152; F=509±27) than in NS (M=375±41; F=233±63) and lower in LS20 (M=97±15; F=76±9) compared to LS10 (M=528±112; F=378±45).Glucose was higher in LS M and F at 5 minutes of the GTT compared to NS offspring. Leptin, insulin, and ITT were not different. But LR methylation was increased in LS20. Conclusion: Sodium restriction during pregnancy leads to low birth weight and to modifications of glucose metabolism and LR methylation.conferenceObject Salt induced cardiac hypertrophy is blood pressure independent and prevented by Losartan and N-acetylcysteine(2013) KATAYAMA, Isis Akemi; PEREIRA, Rafael Canavel; SHIMIZU, Maria Heloisa Massola; HEIMANN, Joel ClaudioconferenceObject Exposure to air pollution before and during pregnancy: Effect on placental structure(2013) SOTO, S. F.; VERAS, M. M.; HEIMANN, J. L.Objective: To evaluate the effects of exposure to air pollution before and during pregnancy. Methods: Female Wistar rats were exposed to filtrated (F) or polluted (P) air in an Atmospheric Particle Concentrator during 3 weeks before pregnancy. At 12 weeks of age, the animals were mated and pregnant females divided in four subgroups (FF, FP, PF and PP). At 19th day of pregnancy, placentas were collected. Placental (Pl) and deciduous (Dc) longitudinal (LD) and transversal (TD) diameters were measured. Results:(mean SEM–p<0.05 mm): 1p<0.05 vs FF; 2p<0.05 vs FF, PF, PP; 3p<0.05 vs FF; 4p<0.05 vs FF, PP; 5p<0.05 vs FF; 6p<0.05 vs PF Conclusions: Exposure to air pollution affects placental structure indicating a possible impairment of fetal nutrition.conferenceObject Low salt intake is associated with maternal obesity before and during pregnancy(2013) TRESOLDI, Michelle Soto; HEIMANN, Joel Claudio; LOPES, Karen LucasechiObjective: To evaluate body weight, adipose tissue mass, serum leptin and mean blood pressure in females fed with different amounts of sodium chloride in three moments of the perinatal period: before (B) and at the end of pregnancy (P) and at the end of lactation (L). Methods: Female Wistar rats were fed low (LS: 0.15%), normal (NS: 1.3%) or high (HS: 8%) salt diet since 8 weeks of age. Mating was induced at 12 weeks of age. The mass of white adipose tissue (WAT, g/100g), brown adipose tissue (BAT, g/100g), body weight (BW, g), serum leptin (SL, μg/mL) by radioimmunoassay, intra-arterial mean blood pressure (BP, mmHg) and adiposity index (AI=WAT/BW x 100, %) were evaluated. Results (p<0.05, values represented by mean±SEM, n=4 to 10/group): In LS fed rats, BAT and SL were lower in L (BAT: 0.11±0.011; LS: 3.6±1.1) compared with B (BAT: 0.18±0.013; LS: 7.7±1.0) and P (BAT: 0.15±0.012; SL: 7.8±1.1). Also in LS fed rats, AI was lower in L (6.1±0.5) compared with B (8.1±0.6) and P (7.8±0.5). In L, BP was higher in HS (137.1±4.9) compared with NS (119.1±2.4) and LS (111.7±7.5). The BW was lower in HS (258.0±6.0) compared with NS (329.0±26.5) and LS (306.5±19.6) group. Conclusions: The amount of dietary salt consumption during the perinatal period influences maternal energy metabolism and blood pressure.