JOEL CLAUDIO HEIMANN
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Clínica Médica, Faculdade de Medicina - Docente
LIM/16 - Laboratório de Fisiopatologia Renal, Hospital das Clínicas, Faculdade de Medicina - Líder
LIM/16 - Laboratório de Fisiopatologia Renal, Hospital das Clínicas, Faculdade de Medicina - Líder
42 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 42
- High maternal sodium intake alters sex-specific renal renin-angiotensin system components in newborn Wistar offspring(2016) MAIA, D. R. R.; LOPES, K. L.; HEIMANN, J. C.; FURUKAWA, L. N. S.This study aimed to evaluate the systemic and renal renin-angiotensin-aldosterone system (RAAS) at birth in male and female offspring and in mothers fed a high sodium diet (HSD) before and during gestation. Female Wistar rats were fed a HSD (8.0% NaCl) or a normal sodium diet (1.3% NaCl) from 8 weeks of age until delivery of their first litter. Maternal body weight, tail blood pressure, and food and water intake were evaluated. The litter sizes were assessed, and the body and kidney weights of the offspring were measured. Both mothers and offspring were euthanized immediately following the birth of the pups to evaluate plasma renin activity (PRA), renal renin content (RRC), renal angiotensin-converting enzyme (ACE) activity, renal angiotensin (Ang) II content, serum aldosterone (ALDO) levels, and renal cortical and medullary renin messenger RNA expression. In mothers in the HSD group, water intake and kidney mass were higher, whereas renal ACE activity, Ang II, PRA, ALDO and RRC were decreased. In the offspring of HSD-fed dams, the body and kidney mass were lower in both genders, renal ACE activity was lower in females and renal Ang II was lower in males. PRA, RRC, renin gene expression and ALDO levels did not differ between the groups of offspring. The data presented herein showed that a maternal HSD during pregnancy induces low birth weight and a sex-specific response in the RAAS in offspring.
- Maternal high-sodium intake alters the responsiveness of the renin-angiotensin system in adult offspring(2012) RAMOS, Debora R.; COSTA, Nauilo L.; JANG, Karen L. L.; OLIVEIRA, Ivone B.; SILVA, Alexandre A. da; HEIMANN, Joel C.; FURUKAWA, Luzia N. S.Aims: The goal of the current study was to evaluate the impact of maternal sodium intake during gestation on the systemic and renal renin-angiotensin-aldosterone-system (RAAS) of the adult offspring. Main methods: Female Wistar rats were fed high- (HSD-8.0% NaCl) or normal-sodium diets (NSD-1.3% NaCl) from 8 weeks of age until the delivery of their first litter. After birth, the offspring received NSD. Tail-cuff blood pressure (TcBP) was measured in the offspring between 6 and 12 weeks of age. At 12 weeks of age, the offspring were subjected to either one week of HSD or low sodium diet (LSD-0.1 6%NaCl) feeding to evaluate RAAS responsiveness or to acute saline overload to examine sodium excretory function. Plasma (PRA) and renal renin content (RRC), serum aldosterone (ALDO) levels, and renal cortical and medullary renin mRNA expression levels were evaluated at the end of the study. Key findings: TcBP was higher among dams fed HSD, but no TcBP differences were observed among the offspring. Male offspring, however, exhibited increased TcBP after one week of HSD feeding, and this effect was independent of maternal diet. Increased RAAS responsiveness to the HSD and LSD was also observed in male offspring. The baseline levels of PRA. ALDO, and cortical and medullary renin gene expression were lower but the RRC levels were higher among HSD-fed male offspring (HSDoff). Conversely, female HSDoff showed reduced sodium excretion 4 h after saline overload compared with female NSDoff. Significance: High maternal sodium intake is associated with gender-specific changes in RAAS responsiveness among adult offspring.
conferenceObject Fine particulate matter in the inspired air within the limits recommended by WHO alters placental structure(2014) SOTO, Sonia; LOPES, Karen; VERAS, Mariana; HEIMANN, JoelconferenceObject Myocardial AT1 gene expression is increased in a model of left ventricular hypertrophy induced by high salt intake(2013) PEREIRA, Rafael Canavel; KATAYAMA, Isis Akemi; HEIMANN, Joel ClaudioObjective: To evaluate gene expression of the renin-angiotensin system components in a model of left ventricular hypertrophy induced by high salt intake demonstrated in a previous study. Methods: Wistar rats were fed normal (NS1.3% NaCl) or high (HS 8%) salt diet since weaning. From the 7th week of age, two HS subgroups received hydralazine or losartan (HZ 15 and LOS 20mg/kg/day). Angiotensinogen, renin, ACE, ACE2, AT1 and AT2 gene expression in left ventricle were measured. Data are reported as mean±SEM. Means in row with superscripts without a common letter differ, P<0.05. Conclusion: High salt intake increases AT1 gene expression, however it is prevented by losartan treatment.conferenceObject Effect of an angiotensin II type 2 receptor agonist on myocardial hypertrophy and fibrosis induced by high salt diet(2017) DOPONA, Ellen Priscila Brito; ROCHA, Veronica Favoni; OLIVEIRA, Ivone Braga de; FURUKAWA, Luzia Naoko Shinohara; HEIMANN, Joel ClaudioconferenceObject Small litter size at birth is associated with insulin resistance and low energy expenditure in adulthood(2013) TAVARES, Neuziane Kloos Amorim; AGUIAR, Fernanda de Jesus; FURUKAWA, Luzia Naoko; HEIMANN, Joel ClaudioObjective: To evaluate the effect of litter size standardization at birth on adult metabolic parameters.Methods: Female Wistar rats became pregnant at 12 weeks of age. Offspring were divided into control(C), G8(8) and G4(4 newborns/litter) groups. At 12 weeks of age, food intake (FI,g/day), glucose (G,mg/dL), insulin (I,pM), cholesterol (Chol,mg/dL), triacylglycerols (TAG,mg/dL) levels, white and brown adipose tissue (BAT) masses (g/100g) adiposity index (AI,% body weight) were determined. Conclusion: Results suggest that changes in litter size is associated with energy balance, carbohydrate and lipid disorders, insulin resistance and alterations in blood pressure in adult male and female.conferenceObject Small litter size at birth is associated with insulin resistance and low energy expenditure in adulthood(2013) TAVARES, N. K. A.; AGUIAR, F. J.; FURUKAWA, L. N. S.; HEIMANN, J. C.Objective: To evaluate the effect of litter size standardization at birth on adult metabolic parameters. Methods: Female Wistar rats were mated and became pregnant at 12 weeks of age. Offspring were divided into control(C), S8(8) and S4(4 newborns/litter) groups. At 12 weeks of age, food intake (FI,g/day), glucose (G,mg/dL), insulin (I,pM), cholesterol (Chol,mg/dL), triacylglycerols (TAG,mg/dL) levels, white and brown adipose tissue (BAT) masses (g/100g) adiposity index (AI,% BW) were determined. Results: (mean±SEM p<0.05, n=6to32)1p<0.05 vs C and S8; 2p<0.05 vs S8 and S4; 3p<0.05 vs C; 4p<0.05 vs S8 Conclusion: Results suggest that the litter size is associated with insulin resistance and low energy expenditure in adulthood.conferenceObject Fine particulate matter in the air within the limits recommended by WHO alters placental structure(2015) SOTO, Sonia; MARCHESI, Guilherme; LOPES, Karen; VERAS, Mariana; HEIMANN, Joel- A novel peptide that improves metabolic parameters without adverse central nervous system effects(2017) RECKZIEGEL, Patricia; FESTUCCIA, William T.; BRITTO, Luiz R. G.; JANG, Karen L. Lopes; ROMAO, Carolina M.; HEIMANN, Joel C.; FOGACA, Manoela V.; RODRIGUES, Naielly S.; SILVA, Nicole R.; GUIMARAES, Francisco S.; EICHLER, Rosangela A. S.; GUPTA, Achla; GOMES, Ivone; DEVI, Lakshmi A.; HEIMANN, Andrea S.; FERRO, Emer S.Intracellular peptides generated by limited proteolysis are likely to function inside and outside cells and could represent new possibilities for drug development. Here, we used several conformational-sensitive antibodies targeting G-protein coupled receptors to screen for novel pharmacological active peptides. We find that one of these peptides, DITADDEPLT activates cannabinoid type 1 receptors. Single amino acid modifications identified a novel peptide, DIIADDEPLT (Pep19), with slightly better inverse agonist activity at cannabinoid type 1 receptors. Pep19 induced uncoupling protein 1 expression in both white adipose tissue and 3T3-L1 differentiated adipocytes; in the latter, Pep19 activates pERK1/2 and AKT signaling pathways. Uncoupling protein 1 expression induced by Pep19 in 3T3-L1 differentiated adipocytes is blocked by AM251, a cannabinoid type 1 receptors antagonist. Oral administration of Pep19 into diet-induced obese Wistar rats significantly reduces adiposity index, whole body weight, glucose, triacylglycerol, cholesterol and blood pressure, without altering heart rate; changes in the number and size of adipocytes were also observed. Pep19 has no central nervous system effects as suggested by the lack of brain c-Fos expression, cell toxicity, induction of the cannabinoid tetrad, depressive-and anxiety-like behaviors. Therefore, Pep19 has several advantages over previously identified peripherally active cannabinoid compounds, and could have clinical applications.
- Salt intake during pregnancy alters offspring's myocardial structure(2013) ALVES-RODRIGUES, E. N.; VERAS, M. M.; ROSA, K. T.; CASTRO, I. de; FURUKAWA, L. N. S.; OLIVEIRA, I. B.; SOUZA, R. M.; HEIMANN, J. C.Background and Aim: To evaluate the effects of low or high salt intake during pregnancy on left ventricle of adult male offspring. Methods and results: Low-(LS, 0.15%), normal-(NS, 1.3%) or high-salt (HS, 8% NaCl) diet was given to Wistar rats during pregnancy. During lactation all dams received NS as well as the offspring after weaning. To evaluate cardiac response to salt overload, 50% of each offspring group was fed a high-salt (hs, 4% NaCl) diet from the 21st to the 36th week of age (LShs, NShs, HShs). The remaining 50% was maintained on NS (LSns, NSns and HSns). Echocardiography was done at 20 and 30 weeks of age. Mean blood pressure (MBP), histology and left ventricular angiotensin II content (AII) were analyzed at 36 weeks of age. Interventricular septum, left ventricular posterior wall and relative wall thickness increased from the 20th to the 30th week of age only in HShs, cardiomyocyte mean volume was higher in HShs compared to NShs, LShs and HSns. AII and left ventricular fibrosis were not different among groups. Conclusions: HS during pregnancy programs adult male offspring to a blood pressure and angiotensin II independent concentric left ventricular hypertrophy, with no fibrosis, in response to a chronic high-salt intake.