CLARICE ROSA OLIVO
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
LIM/20 - Laboratório de Terapêutica Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/20 - Laboratório de Terapêutica Experimental, Hospital das Clínicas, Faculdade de Medicina
12 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 12
- A possible association between fructose consumption and pulmonary emphysema (vol 9, 9344, 2019)(2020) SUEHIRO, Camila Liyoko; TOLEDO-ARRUDA, Alessandra Choqueta de; VIEIRA, Rodolfo de Paula; ALMEIDA, Francine Maria de; OLIVO, Clarice Rosa; MARTINS, Milton de Arruda; LIN, Chin Jia
- Dehydrodieugenol improved lung inflammation in an asthma model by inhibiting the STAT3/SOCS3 and MAPK pathways(2020) SANTANA, Fernanda P. R.; SILVA, Rafael C. da; PONCI, Vitor; PINHEIRO, Aruana J. M. C. R.; OLIVO, Clarice R.; CAPERUTO, Luciana C.; ARANTES-COSTA, Fernanda M.; CLAUDIO, Samuel R.; RIBEIRO, Daniel A.; TIBERIO, Iolanda F. L. C.; LIMA-NETO, Lidio G.; LAGO, Joao Henrique G.; PRADO, Carla M.Background: Eugenol, a common phenylpropanoid derivative found in different plant species, has well-described anti-inflammatory effects associated with the development of occupational hypersensitive asthma. Dehydrodieugenol, a dimeric eugenol derivative, exhibits anti-inflammatory and antioxidant activities and can be found in the Brazilian plant species Nectandra leucantha (Lauraceae). The biological effects of dehydrodieugenol on lung inflammation remain unclear. Purpose: This study aimed to investigate the effects of eugenol and dehydrodieugenol isolated from N. leucantha in an experimental model of asthma. Methods: In the present work, the toxic effects of eugenol and dehydrodieugenol on RAW 264.7 cells and their oxidant and inflammatory effects before lipopolysaccharide (LPS) exposure were tested. Then, male BALB/c mice were sensitized with ovalbumin through a 29-day protocol and treated with vehicle, eugenol, dehydrodieugenol or dexamethasone for eight days beginning on the 22nd day until the end of the protocol. Lung function; the inflammatory profile; and the protein expression of ERK1/2, JNK, p38, VAChT, STAT3, and SOCS3 in the lung were evaluated by immunoblotting. Results: Eugenol and dehydrodieugenol were nontoxic to cells. Both compounds inhibited NO release and the gene expression of IL-1 beta and IL-6 in LPS-stimulated RAW 264.7 cells. In OVA-sensitized animals, dehydrodieugenol reduced lung inflammatory cell numbers and the lung concentrations of IL-4, IL-13, IL-17, and IL-10. These anti-inflammatory effects were associated with inhibition of the JNK, p38 and ERK1/2, VAChT and STAT3/SOCS3 pathways. Moreover, treatment with dehydrodieugenol effectively attenuated airway hyperresponsiveness. Conclusion: The obtained data demonstrate, for the first time, that dehydrodieugenol was more effective than eugenol in counteracting allergic airway inflammation in mice, especially its inhibition of the JNK, p38 and ERK1/2, components of MAPK pathway. Therefore, dehydrodieugenol can be considered a prototype for the development of new and effective agents for the treatment of asthmatic patients.
- Inflammatory response to Streptococcus pneumoniae was attenuated by moderate aerobic exercise training(2020) CASTRO, Thamyres; LAIA, Roseane M.; MENDONCA, Luana D.; TRINDADE, Pietro Rocha; SOUZA, Jessica C.; SARAIVA-ROMANHOLO, Beatriz M.; OLIVEIRA, Maria Leonor S.; MIYAGI, Eliane N.; PRADO, Carla M.; TIBERIO, Iolanda F. C. L.; OLIVO, Clarice Rosa
- The deleterious effects of smoking in bone mineralization and fibrillar matrix composition(2020) BARBOSA, Alexandre Povoa; LOURENCO, Juliana Dias; JUNQUEIRA, Jader Joel Machado; FRANCA, Silva Larissa Emidio de; MARTINS, Janaina S.; OLIVEIRA JUNIOR, Manoel Carneiro; BEGALLI, Isadora; VELOSA, Ana Paula Pereira; OLIVO, Clarice Rosa; BASTOS, Thiago Bernardes; JORGETTI, Vanda; PAULA, Vieira Rodolfo de; TEODORO, Walcy Rosolia; LOPES, Fernanda D. T. Q. S.Introduction: This study aimed to verify the effects of cigarette smoke exposure in bone mineralization and fibrillar matrix composition as well as in bone healing after tibial fracture induction. Methods: C57Bl/6 Mice were assigned according to exposure and surgery: C room air; F room air and tibia open osteotomy; CS cigarette smoke; FCS cigarette smoke and tibia open osteotomy. In order to study fracture healing we performed, under anesthesia, a bone injury through a tibial shaft osteotomy. Bone samples were obtained to evaluate bone histomorphometry, trabecular morphology and volume, trabecular collagen types composition and presence of inflammatory cytokines and growth factors. Results: CS exposure significantly reduced the thickness of bone trabeculae associated with decrease in mineralizing surface and mineral deposition rate, leading a lower bone formation rate and longer mineralization time. Resorption surface and osteoclastic surface were greater in the CS group, attesting increased resorptive action. There was a decrease in type I collagen deposition and genes expression in the CS and FCS groups compared to C group and in contrast there was an increase in type V collagen deposition and genes expression in the CS, FC and FSC groups compared to C group. Also, CS exposure induced a decrease in bone forming cytokines and an increase in inflammatory associated cytokines, and these changes were intensified under fracture conditions. Conclusion: Cigarette smoke exposure alters bone matrix composition and worsens bone mineralization, leading to bone fragility by increasing collagen V synthesis and deposition and impairing collagen I fibril forming and assembling. And these deleterious effects contributed to the worsening in fracture healing after tibia osteotomy.
- High Intensity Interval Training control inflammation in mice exposed to Streptococus pneumoniae(2020) CASTRO, Thamyres B. P.; LAIA, Roseane M.; MENDONCA, Luana D.; TRINDADE, Pietro Rocha; SOUZA, Jessica C.; SARAIVA-ROMANHOLO, Beatriz M.; OLIVEIRA, Maria Leonor S.; MIYAGI, Eliane N.; PRADO, Carla M.; TIBERIO, Iolanda F. C. L.; OLIVO, Clarice Rosa
- Biseugenol Exhibited Anti-Inflammatory and Anti-Asthmatic Effects in an Asthma Mouse Model of Mixed-Granulocytic Asthma(2020) PONCI, Vitor; SILVA, Rafael C.; SANTANA, Fernanda Paula R.; GRECCO, Simone S.; FORTUNATO, Celia Regina M.; OLIVEIRA, Maria A.; TAVARES-DE-LIMA, Wothan; OLIVO, Clarice R.; TIBERIO, Iolanda de Fatima L. Calvo; GOMES, Kaio S.; PRADO, Carla M.; LAGO, Joao Henrique G.In the present work, the anti-inflammatory and antiasthmatic potential of biseugenol, isolated as the main component from n-hexane extract from leaves of Nectandra leucantha and chemically prepared using oxidative coupling from eugenol, was evaluated in an experimental model of mixed-granulocytic asthma. Initially, in silico studies of biseugenol showed good predictions for drug-likeness, with adherence to Lipinski's rules of five (RO5), good Absorption, Distribution, Metabolism and Excretion (ADME) properties and no alerts for Pan-Assay Interference Compounds (PAINS), indicating adequate adherence to perform in vivo assays. Biseugenol (20 mg center dot kg(-1)) was thus administered intraperitoneally (four days of treatment) and resulted in a significant reduction in both eosinophils and neutrophils of bronchoalveolar lavage fluid in ovalbumin-sensitized mice with no statistical difference from dexamethasone (5 mg center dot kg(-1)). As for lung function parameters, biseugenol (20 mg center dot kg(-1)) significantly reduced airway and tissue damping in comparison to ovalbumin group, with similar efficacy to positive control dexamethasone. Airway hyperresponsiveness to intravenous methacholine was reduced with biseugenol but was inferior to dexamethasone in higher doses. In conclusion, biseugenol displayed antiasthmatic effects, as observed through the reduction of inflammation and airway hyperresponsiveness, with similar effects to dexamethasone, on mixed-granulocytic ovalbumin-sensitized mice.
- High intensity interval training control inflammation in a lung allergic model(2020) TRINDADE, Pietro da Rocha; CASTELO, Thais F. F.; CASTRO, Thamyres B. P.; LAIA, Roseane M.; MENDONCA, Luana D.; SOUZA, Jessica C.; SARAIVA-ROMANHOLO, Beatriz M.; PRADO, Carla M.; TIBERIO, Iolanda F. C. L.; OLIVO, Clarice Rosa
conferenceObject Treatment with Dehydrodieugenol B Ameliorates LPS-Induced Acute Lung Injury by Reducing Oxidative Stress(2020) PINHEIRO, N.; BITTENCOURT-MERNAK, M.; SILVA, R.; PONCI, V.; PINHEIRO, A.; OLIVO, C. R.; TIBERIO, I.; NETO, L.; LAGO, J.; SANTANA, F. R.; PRADO, C. M.- Effects of VAChT reduction and alpha 7nAChR stimulation by PNU-282987 in lung inflammation in a model of chronic allergic airway inflammation(2020) PINHEIRO, Nathalia M.; MIRANDA, Claudia J. C. P.; SANTANA, Fernanda R.; BITTENCOURT-MERNAK, Marcia; ARANTES-COSTA, Fernanda M.; OLIVO, Clarice; PERINI, Adenir; FESTA, Sergio; CAPERUTO, Luciana C.; TIBERIO, Iolanda F. L. C.; PRADO, Marco Antonio M.; MARTINS, Milton A.; PRADO, Vania F.; PRADO, Carla M.The cholinergic anti-inflammatory pathway has been shown to regulate lung inflammation and cytokine release in acute models of inflammation, mainly via alpha 7 nicotinic receptor (alpha 7nAChR). We aimed to evaluate the role of endogenous acetylcholine in chronic allergic airway inflammation in mice and the effects of therapeutic nAChR stimulation in this model. We first evaluated lung inflammation and remodeling on knock-down mice with 65% of vesicular acetylcholine transport (VAChT) gene reduction (KDVAChT) and wild-type(WT) controls that were subcutaneously sensitized and then inhaled with ovalbumin(OVA). We then evaluated the effects of PNU282987(0.5-to-2mg/kg),( alpha 7nAChR agonist) treatment in BALB/c male mice intraperitoneal sensitized and then inhaled with OVA. Another OVA-sensitized-group was treated with PNU-282987 plus Methyllycaconitine (MLA,1 mg/kg, alpha 7nAChR antagonist) to confirm that the effects observed by PNU were due to alpha 7nAChR. We showed that KDVAChT-OVA mice exhibit exacerbated airway inflammation when compared to WT-OVA mice. In BALB/c, PNU-282987 treatment reduced the number of eosinophils in the blood, BAL fluid, and around airways, and also decreased pulmonary levels of IL-4,IL-13,IL-17, and IgE in the serum of OVA-exposed mice. MLA pretreatment abolished all the effects of PNU-282987. Additionally, we showed that PNU-282987 inhibited STAT3phosphorylation and reduced SOCS3 expression in the lung. These data indicate that endogenous cholinergic tone is important to control allergic airway inflammation in a murine model. Moreover, alpha 7nAChR is involved in the control of eosinophilic inflammation and airway remodeling, possibly via inhibition of STAT3/SOCS3 pathways. Together these data suggest that cholinergic anti-inflammatory system mainly alpha 7nAChR should be further considered as a therapeutic target in asthma.
- Aerobic exercise training attenuates detrimental effects of cigarette smoke exposure on peripheral muscle through stimulation of the Nrf2 pathway and cytokines: a time-course study in mice(2020) TOLEDO-ARRUDA, Alessandra C.; SOUSA NETO, Ivo Vieira de; VIEIRA, Rodolfo P.; GUARNIER, Flavia A.; CALEMAN-NETO, Agostinho; SUEHIRO, Camila L.; OLIVO, Clarice R.; CECCHINI, Rubens; PRADO, Carla M.; LIN, Chin J.; DURIGAN, Joao Luiz Quaglioti; MARTINS, Milton A.Cigarette smoke (CS) exposure reduces skeletal muscle function; however, the mechanisms involved have been poorly investigated. The current study evaluated the temporal effects of aerobic exercise training on oxidant and antioxidant systems as well as inflammatory markers in skeletal muscle of mice exposed to CS. Mice were randomly allocated to control, exercise, smoke, and smoke+exercise groups and 3 time points (4, 8, and 12 weeks; n = 12 per group). Exercise training and CS exposure were performed for 30 min/day, twice a day, 5 days/week for 4, 8, and 12 weeks. Aerobic exercise improved functional capacity and attenuated the increase in the cachexia index induced by CS exposure after 12 weeks. Concomitantly, exercise training downregulated tumor necrosis factor a concentration, glutathione oxidation, and messenger RNA (mRNA) expression of Keap1 (P < 0.01) and upregulated interleukin 10 concentration, total antioxidant capacity, and mRNA expression of Nrf2, Gsr, and Txn1 (P < 0.01) in muscle. Exercise increased mRNA expression of Hmox1 compared with the control after 12 weeks (P < 0.05). There were no significant differences between smoke groups for superoxide dismutase activity and Hmox1 mRNA expression. Exercise training improved the ability of skeletal muscle to adequately upregulate key antioxidant and anti-inflammatory defenses to detoxify electrophilic compounds induced by CS exposure, and these effects were more pronounced after 12 weeks. Novelty Exercise attenuates oxidative stress in skeletal muscle from animals exposed to CS via Nrf2 and glutathione pathways. Exercise is a helpful tool to control the inflammatory balance in skeletal muscle from animals exposed to CS. These beneficial effects were evident after 12 weeks.