MARCONY RODRIGUES DE SANTHIAGO
Projetos de Pesquisa
Unidades Organizacionais
LIM/33 - Laboratório de Oftalmologia, Hospital das Clínicas, Faculdade de Medicina
1 resultados
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- Corneal stromal localization of TGF beta isoforms in spontaneous persistent epithelial defects after PRK in rabbits(2024) VILLABONA-MARTINEZ, Valeria; DUTRA, Barbara A. L.; SAMPAIO, Lycia P.; SANTHIAGO, Marcony R.; WILSON, Steven E.The purpose of this study was to evaluate transforming growth factor beta (TGFI3) isoform localization in rabbit corneas with spontaneous persistent epithelial defects (PEDs) after photorefractive keratectomy (PRK). Four cryofixed corneas from a previously reported series of PEDs in rabbits that had PRK were evaluated with triplex immunohistochemistry (IHC) for TGFI33, myofibroblast marker alpha-smooth muscle actin (alpha-SMA) and mesenchymal marker vimentin. One cornea had sufficient remaining tissue for triplex IHC for TGFI31, TGFI32, or TGFI33 (each with alpha-SMA and vimentin) using isoform-specific antibodies. All three TGFI3 isoforms were detected in the subepithelial stroma at and surrounding the PED. Some of each TGFI3 isoform co-localized with alpha-SMA of myofibroblasts, which could be TGFI3 isoform autocrine production by myofibroblasts or TGFI3-1, -2, and -3 binding to these myofibroblasts.