BRUNO TADEU MARTINS DE OLIVEIRA
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
LIM/20 - Laboratório de Terapêutica Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/20 - Laboratório de Terapêutica Experimental, Hospital das Clínicas, Faculdade de Medicina
8 resultados
Resultados de Busca
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conferenceObject Plant proteinase from Bauhinia bauhinioides Kallikrein inhibitor (BbKI) attenuates mechanics, inflammation and remodelling induced by elastase in mice(2012) OLIVEIRA, Bruno Martins; ALMEIDA-REIS, Rafael; THEODORO, Osmar A.; OLIVA, Leandro V.; RODRIGUES, Daniel Flisch; PINHEIRO, Nathalia; OLIVA, Maria L. V.; PRADO, Carla M.; MARTINS, Milton M.; TIBERIO, Iolanda F. L. C.conferenceObject Plant proteinase inhibitor from Enterolobium contortisiliquum (EcTI) attenuates elastase-induced pulmonary inflammatory, remodeling and mechanical alterations in mice(2012) THEODORO-JUNIOR, Osmar A.; MARQUES, Ricardo Henrique; ALMEIDA-REIS, Rafael; MARTINS-OLIVEIRA, Bruno T.; OLIVA, Leandro V.; LOPES, Tales; PRADO, Carla M.; OLIVA, Maria L. V.; MARTINS, Milton; TIBERIO, Iolanda F. L. C.- Plant Proteinase Inhibitor BbCI Modulates Lung Inflammatory Responses and Mechanic and Remodeling Alterations Induced by Elastase in Mice(2017) ALMEIDA-REIS, Rafael; THEODORO-JUNIOR, Osmar A.; OLIVEIRA, Bruno T. M.; OLIVA, Leandro V.; TOLEDO-ARRUDA, Alessandra C.; BONTURI, Camila R.; BRITO, Marlon V.; LOPES, Fernanda D. T. Q. S.; PRADO, Carla M.; FLORENCIO, Ariana C.; MARTINS, Milton A.; OWEN, Caroline A.; LEICK, Edna A.; OLIVA, Maria L. V.; TIBERIO, Iolanda F. L. C.Background. Proteinases play a key role in emphysema. Bauhinia bauhinioides cruzipain inhibitor (BbCI) is a serine-cysteine proteinase inhibitor. We evaluated BbCI treatment in elastase-induced pulmonary alterations. Methods. C57BL/6 mice received intratracheal elastase (ELA group) or saline (SAL group). One group of mice was treated with BbCI (days 1, 15, and 21 after elastase instillation, ELABC group). Controls received saline and BbCI (SALBC group). After 28 days, we evaluated respiratory mechanics, exhaled nitric oxide, and bronchoalveolar lavage fluid. In lung tissue we measured airspace enlargement, quantified neutrophils, TNF alpha-, MMP-9-, MMP-12-, TIMP-1-, iNOS-, and eNOS-positive cells, 8-iso-PGF2 alpha, collagen,and elastic fibers in alveolar septa and airways. MUC-5-positive cells were quantified only in airways. Results. BbCI reduced elastase-induced changes in pulmonary mechanics, airspace enlargement and elastase-induced increases in total cells, and neutrophils in BALF. BbCI reduced macrophages and neutrophils positive cells in alveolar septa and neutrophils and TNF alpha-positive cells in airways. BbCI attenuated elastic and collagen fibers, MMP-9- and MMP-12-positive cells, and isoprostane and iNOS-positive cells in alveolar septa and airways. BbCI reduced MUC5ac-positive cells in airways. Conclusions. BbCI improved lung mechanics and reduced lung inflammation and airspace enlargement and increased oxidative stress levels induced by elastase. BbCI may have therapeutic potential in chronic obstructive pulmonary disease.
- A plant proteinase inhibitor from Crataeva tapia (CrataBL) attenuates elastase-induced pulmonary inflammatory, remodeling, and mechanical alterations in mice(2015) OLIVA, Leandro V.; ALMEIDA-REIS, Rafael; THEODORO-JUNIOR, Osmar; OLIVEIRA, Bruno M.; LEICK, Edna A.; PRADO, Carla M.; BRITO, Marlon V.; CORREIA, Maria Tereza dos Santos; PAIVA, Patricia M. G.; MARTINS, Milton A.; OLIVA, Maria Luiza V.; TIBERIO, Iolanda F. L. C.Chronic obstructive pulmonary disease (COPD) can lead to chronic and obstructive bronchitis and emphysema resulting in decreased bronchial lumen size. This study evaluated the effect of CrataBL, a protein isolated from the bark of Crataeva tapia, on lung mechanics, inflammation, and remodeling after elastase-induced pulmonary alterations in mice. The use of CrataBL led to decreased mechanical alterations, alveolar septum disruption (Lm), number of macrophage and neutrophil cells in the BALF, and TNF-alpha, MMP-9, MMP-12, TIMP-1, eNOS, and iNOS positive cells in the airways and alveolar walls compared to the animals in the ELA group. Moreover, a reduction in MUC-5-positive cells in the airway walls was observed. In conclusion, CrataBL attenuates changes in lung mechanics, inflammation, extracellular lung remodeling, and oxidative stress responses induced by the administration of elastase and decreased the volume fraction of isoprostane, collagen, and elastic fibers in the airways and alveolar walls compared to the animals in the ELA groups. Therefore, CrataBL is a potential therapeutic tool in the treatment of COPD.
- A Plant Proteinase Inhibitor from Enterolobium contortisiliquum Attenuates Pulmonary Mechanics, Inflammation and Remodeling Induced by Elastase in Mice(2017) THEODORO-JUNIOR, Osmar Aparecido; RIGHETTI, Renato Fraga; ALMEIDA-REIS, Rafael; MARTINS-OLIVEIRA, Bruno Tadeu; OLIVA, Leandro Vilela; PRADO, Carla Maximo; SARAIVA-ROMANHOLO, Beatriz Mangueira; LEICK, Edna Aparecida; PINHEIRO, Nathalia Montouro; LOBO, Yara Aparecida; MARTINS, Milton de Arruda; OLIVA, Maria Luiza Vilela; TIBERIO, Iolanda de Fatima Lopes CalvoProteinase inhibitors have been associated with anti-inflammatory and antioxidant activities and may represent a potential therapeutic treatment for emphysema. Our aim was to evaluate the effects of a plant Kunitz proteinase inhibitor, Enterolobium contortisiliquum trypsin inhibitor (EcTI), on several aspects of experimental elastase-induced pulmonary inflammation in mice. C57/Bl6 mice were intratracheally administered elastase (ELA) or saline (SAL) and were treated intraperitoneally with EcTI (ELA-EcTI, SAL-EcTI) on days 1, 14 and 21. On day 28, pulmonary mechanics, exhaled nitric oxide (ENO) and number leucocytes in the bronchoalveolar lavage fluid (BALF) were evaluated. Subsequently, lung immunohistochemical staining was submitted to morphometry. EcTI treatment reduced responses of the mechanical respiratory system, number of cells in the BALF, and reduced tumor necrosis factor- (TNF-), matrix metalloproteinase-9 (MMP-9), matrix metalloproteinase-12 (MMP-12), tissue inhibitor of matrix metalloproteinase (TIMP-1), endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS)-positive cells and volume proportion of isoprostane, collagen and elastic fibers in the airways and alveolar walls compared with the ELA group. EcTI treatment reduced elastase induced pulmonary inflammation, remodeling, oxidative stress and mechanical alterations, suggesting that this inhibitor may be a potential therapeutic tool for chronic obstructive pulmonary disease (COPD) management.
conferenceObject Treatment with proteinase inhibitor, BbCI, modulates inflammatory response, mechanic alterations, and remodeling on elastase-induced emphysema in mice(2012) ALMEIDA-REIS, Rafael; OLIVEIRA, Bruno T. M.; THEODORO-JUNIOR, Osmar A.; OLIVA, Leandro V.; PINHEIRO, Nathalia; BRITO, Marlon V.; LOPES, Fernanda D. Q. T.; PRADO, Carla M.; MARTINS, Milton A.; OLIVA, Maria Luiza V.; TIBERIO, Iolanda F. L. C.conferenceObject Plant proteinase from bauhinia bauhinioides kallikrein inhibitor (BbKI) attenuates inflammation and remodelling induced by elastase in mice(2013) LEICK, Edna A.; OLIVEIRA, Bruno Tadeu Martins; ALMEIDA-REIS, Rafael; OLIVA, Leandro Vilela; THEODORO JR., Osmar A.; PRADO, Carla Maximo; MARTINS, Milton A.; OLIVA, Maria Luiza Vilela; TIBERIO, Iolanda F. L. C.conferenceObject Plant Kunitz proteinase inhibitor allenuates inflammation, oxidative stress and remodeling induced by elastase in mice(2014) LEICK, Edna K.; THEODORO-JUNIOR, Osmar A.; RIGHETTI, Renato F.; ALMEIDA-REIS, Rafael; OLIVA, Leandro; OLIVEIRA, Bruno T. Martins; PINHEIRO, Natalia M.; PRADO, Carla Maximo; OLIVA, Maria Luiza Vilela; MARTINS, Milton A.; TIBERIO, Iolanda F. L. C.