ALEXANDRE LEOPOLD BUSSE
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina - Médico
LIM/66, Hospital das Clínicas, Faculdade de Medicina
LIM/66, Hospital das Clínicas, Faculdade de Medicina
6 resultados
Resultados de Busca
Agora exibindo 1 - 6 de 6
- Evaluation of the diaphragm muscle remodeling, inflammation, oxidative stress and vascularization in smokers: An autopsy study(2020) NUCCI, R.A.B.; MAIFRINO, L.B.M.; BUSSE, A.L.; SOUZA, R.R. De; PASQUALUCCI, C.A.; ANARUMA, C.A.; LEITE, R.E.P.; RODRIGUEZ, R.D.; SUEMOTO, C.K.; JACOB-FILHO, W.Background/Aims: Cigarette smoking is a key factor in systemic inflammation and oxidative stress, and it has also been associated with the loss of muscle strength and an elevated risk of pulmonary diseases. Thus, this study aimed to analyze the effects of cigarette smoking on the diaphragm muscle structure of postmortem samples. Methods: Immunohistochemical techniques were used for muscle remodeling (metalloproteinases 2 and 9), inflammation (cyclooxygenase-2), oxidative stress (8-hydroxy-2'-deoxyguanosine), and vascularization (vascular endothelial growth factor). Hematoxylin and eosin stain was used for histopathological analysis and Picrosirius stain was used to highlight the collagen fibers. Results: Cigarette smokers had an increase of diaphragm muscle remodeling, oxidative stress, inflammation, and vascularization compared to non-smokers. Conclusion: Diaphragm muscle structure may be negatively affected by cigarette smoking. © 2020 The Author(s).
- Association of Cognitive Performance with Frailty in Older Individuals with Cognitive Complaints(2022) LIN, S. M.; APOLINARIO, D.; GOMES, G. C. Vieira; TOSI, F. Cassales; MAGALDI, R. M.; BUSSE, A. L.; GIL, G.; RIBEIRO, E.; SATOMI, E.; APRAHAMIAN, I.; FILHO, W. J.; SUEMOTO, Claudia K.Objectives Frailty is a risk factor for poor cognitive performance in older adults. However, few studies have evaluated the association of cognitive performance with frailty in a low- to middle-income country (LMIC). This study aimed to investigate an association between cognitive performance and frailty in older adults with memory complaints in Brazil. Secondarily, we aim to assess an association of cognitive performance with gait speed and grip strength. Design Cross-sectional study. Setting Outpatient service from a LMIC Participants Older adults with memory complaints reported by the participants, their proxies, or their physicians. Measurements Frailty was evaluated using the Cardiovascular Health Study criteria. A neuropsychological battery evaluated memory, attention, language, visuospatial function, executive function. Linear regression analysis with adjustment for age, sex, and education was used. We also evaluated the interaction of education with frailty, grip strength, and gait speed. Results Prefrailty was associated with poor performance in the memory domain, as well as slower gait speed was associated with worse performance in memory, attention, language, and executive function. Frailty and grip strength were not associated with cognitive performance. Interactions of education with gait speed were significant for global performance, as well as for attention and visuospatial ability. Conclusion In elderly patients with memory complaints, prefrailty was associated with poor memory performance. Slowness was associated with poorer performance in some cognitive domains, mainly in participants with low education.
- Diaphragm muscle structure in the elderly: Findings from an autopsy study(2020) NUCCI, Ricardo Aparecido Baptista; SOUZA, Romeu Rodrigues de; SUEMOTO, Claudia Kimie; BUSSE, Alexandre Leopold; MAIFRINO, Laura Beatriz Mesiano; ANARUMA, Carlos Alberto; PASQUALUCCI, Carlos Augusto; JACOB-FILHO, WilsonSarcopenia is characterized as aging-related weakness and atrophy, which decreases the diaphragm force generating capacity leading to a decrease of expulsive non-ventilatory motor behaviors, which are critical for airway clearance. Thus, this study aimed to analyze the histopathology of the diaphragm in postmortem samples. Thirty individuals were included. Diaphragm samples were stained with hematoxylin and eosin for histopathological analysis. Picrosirius stain was used to highlight the collagen fibers. We observed a positive association between advancing age and histopathological findings in the diaphragm structure. We suggest that age is a key factor in increasing diaphragm muscle histopathology. However, further clinicopathological studies are needed to confirm our findings.
- Smoking Intensity Increases Diaphragm Muscle Injury: A Clinicopathologic Study(2023) NUCCI, Ricardo Aparecido Baptista; BUSSE, Alexandre Leopold; SOUZA, Romeu Rodrigues de; MAIFRINO, Laura Beatriz Mesiano; PASQUALUCCI, Carlos Augusto; ANARUMA, Carlos Alberto; LEITE, Renata Elaine Paraizo; RODRIGUEZ, Roberta Diehl; SUEMOTO, Claudia Kimie; JACOB-FILHO, WilsonBackground: Studying the effects of smoking intensity is important to evaluate the risk of tobacco use on a range of illnesses, such as as sarcopenia among the elderly. Thus, this study aimed to analyze the effects of pack-years of cigarette smoking on the diaphragm muscle (DIAm) histopathology of postmortem samples. Methods: Subjects were divided into three groups: never-smoker (n = 46); less than 30 pack-years of smoking (n = 12); and more than 30 pack-years of smoking (n = 30). Diaphragm samples were stained with Picrosirius red and hematoxylin and eosin stain for general structure. Results: Participants with more than 30 pack-years of cigarette smoking had a significant increase in adipocytes, blood vessels and collagen deposit, as well as an increase in histopathological alterations. Conclusions: Pack-years of smoking was associated with DIAm injury. However, further clinicopathological studies are needed to confirm our findings.
- Characterizing spontaneously reported cognitive complaints: the development and reliability of a classification instrument(2013) APOLINARIO, Daniel; MIRANDA, Rafaela Branco; SUEMOTO, Claudia Kimie; MAGALDI, Regina Miksian; BUSSE, Alexandre Leopold; SOARES, Aline Thomaz; LOPES, Leonardo da Costa; KASAI, Juliana Yumi Tizon; SATOMI, Erika; KIKUCHI, Elina Lika; JACOB-FILHO, WilsonBackground: The characteristics and associated risks of spontaneously reported cognitive complaints have not been investigated due to the lack of a classification instrument. Methods: In phase 1, a classification system with descriptive categories and cognitive domains was developed by experts through a modified Delphi technique. In phase 2, 180 elderly patients seeking medical attention for cognitive complaints provided free reports of their cognitive difficulties and each complaint was recorded verbatim. Three observers were asked to classify each complaint into a descriptive category. Perceived cognitive function was further characterized using the Memory Complaint Questionnaire (MAC-Q). Results: The patients reported 493 spontaneous complaints, with a range of 1-6 complaints per patient and a mean of 2.7 (+/- 1.3). The proportion of complaints that could be classified into a category by each of the three observers varied from 91.9% to 95.7%. Inter-observer agreement assessed using the kappa statistic varied from 0.79 to 1 for descriptive categories and 0.83 to 0.97 for domains. Compared with the MAC-Q, spontaneously reported complaints provided complementary information by avoiding the cueing effect provoked by the questionnaire. The total number of complaints and their occurrences in specific domains were associated with important sociodemographic and clinical factors, indicating that their meaning and associated risks need to be further investigated. Conclusion: The instrument developed in this study proved to be a practical tool for classifying the majority of spontaneously reported cognitive complaints with high reliability. Further studies are needed to investigate clinical usefulness of this approach.
- Advancing cognitive assessment in telemedicine: Validity and reliability of the telephone 10-point cognitive screener(2023) RICO, Bruno Marcondes Hottum; ALIBERTI, Marlon Juliano Romero; SILVA, Natalia Oliveira Trajano da; BUSSE, Alexandre Leopold; SUEMOTO, Claudia Kimie; AVELINO-SILVA, Thiago Junqueira; MAGALDI, Regina Miksian; APOLINARIO, Daniel