MARIA MITZI BRENTANI
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Radiologia, Faculdade de Medicina - Docente
LIM/24 - Laboratório de Oncologia Experimental, Hospital das Clínicas, Faculdade de Medicina - Líder
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/24 - Laboratório de Oncologia Experimental, Hospital das Clínicas, Faculdade de Medicina - Líder
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina
9 resultados
Resultados de Busca
Agora exibindo 1 - 9 de 9
- Claudin 4 and 7 Expression Is Not Correlated to Triple Negative Phenotype in Invasive NOS Breast Carcinomas: A Wide Series Assessed by Immunohistochemistry(2014) LOGULLO, A. F.; STIEPCICH, M. A.; NONOGAKI, S.; MALAGOLI, R.; BRENTANI, M. M.; PASINI, F. S.; SOARES, F. A.
- MicroRNAs Discriminate Familial from Sporadic Non-BRCA1/2 Breast Carcinoma Arising in Patients <= 35 Years(2014) BASTOS, Elen Pereira; BRENTANI, Helena; PASINI, Fatima Solange; SILVA, Aderbal Ruy T.; TORRES, Cesar Henrique; PUGA, Renato David; OLIVIERI, Eloisa Helena Ribeiro; PIOVEZANI, Amanda Rusiska; PEREIRA, Carlos Alberto de Braganca; MACHADO-LIMA, Ariane; CARRARO, Dirce Maria; BRENTANI, Maria MitziThe influence of genetic factors may contribute to the poor prognosis of breast cancer (BC) at a very young age. However BRCA1/2 mutations could not explain the majority of cases arising in these patients. MicroRNAs (miRs) have been implicated in biological processes associated with BC. Therefore, we investigated differences in miRs expression between tumors from young patients (<= 35 years) with sporadic or familial history and non-carriers of BRCA1/2 mutations. Thirty-six young Brazilian patients were divided into 2 groups: sporadic (NF-BC) or familial breast cancer (F-BC). Most of the samples were classified as luminal A and B and the frequency of subtypes did not differ between familial or sporadic cases. Using real time qPCR and discriminant function analysis, we identified 9 miRs whose expression levels rather than miR identity can discriminate between both patient groups. Candidate predicted targets were determined by combining results from miRWalk algorithms with mRNA expression profiles (n = 91 differently expressed genes). MiR/mRNA integrated analysis identified 91 candidate genes showing positive or negative correlation to at least 1 of the 9 miRs. Co-expression analysis of these genes with 9 miRs indicated that 49 differentially co-expressed miR-gene interactions changes in F-BC tumors as compared to those of NF-BC tumors. Out of 49, 17 (34.6%) of predicted miR-gene interactions showed an inverse correlation suggesting that miRs act as post-transcriptional regulators, whereas 14 (28.6%) miR-gene pairs tended to be co-expressed in the same direction indicating that the effects exerted by these miRs pointed to a complex level of target regulation. The remaining 18 pairs were not predicted by our criteria suggesting involvement of other regulators. MiR-mRNA co-expression analysis allowed us to identify changes in the miR-mRNA regulation that were able to distinguish tumors from familial and sporadic young BC patients non-carriers of BRCA mutations.
- Claudin 4 and 7 Expression Is Not Correlated to Triple Negative Phenotype in Invasive NOS Breast Carcinomas: A Wide Series Assessed by Immunohistochemistry(2014) LOGULLO, A. F.; STIEPCICH, M. A.; NONOGAKI, S.; MALAGOLI, R.; BRENTANI, M. M.; PASINI, F. S.; SOARES, F. A.
- Calcitriol supplementation effects on Ki67 expression and transcriptional profile of breast cancer specimens from post-menopausal patients(2014) URATA, Yuri Nagamine; LYRA, Eduardo Carneiro de; KATAYAMA, Maria Lucia Hirata; BASSO, Ricardo Alves; ASSIS, Paulo Eduardo Zuccolotto de; CARDOSO, Ana Paula Torres; ROELA, Rosimeire Aparecida; NONOGAKI, Suely; GOES, Joao Carlos Guedes Sampaio; BRENTANI, M. Mitzi; FOLGUEIRA, Maria Aparecida Azevedo KoikeBackground & aims: High concentration of 1,25(OH)(2)D-3 (50-100 nM), which cause hypercalcemia in vivo, induce the hormone transcriptional targets and exert antiproliferative effects in cultured breast cancer lineages, however, no studies investigated whether these effects might be reproduced in tumor specimens in vivo. Our aim was to evaluate the effects of calcitriol supplementation on the proliferative index (Ki67 expression) and gene expression profile of post-menopausal breast cancer samples. Methods & results: Tumor samples were collected from 33 patients, most of whom (87.5%) presenting 25(OH)D-3 insufficiency, before and after a short term calcitriol supplementation (0.50 mu g/day PO, for 30 days). Tumor dimension remained stable in ultrasound evaluations. A slight reduction in Ki67 immunoexpression was detected, however in only 10/32 post-calcitriol samples an expressively low proliferative index [Ln (%Ki67+) < 1] was achieved. Gene expression from 15 matched pre/post-supplementation samples was analyzed by microarray (U133 Plus 2.0 GeneChip, Affymetrix) and 15 genes were over-expressed in post-supplementation tumors, including FOS and EGR1, which were previously shown to be regulated by vitamin D. However, these results were not confirmed in another four breast cancer samples. Conclusions: Calcitriol supplementation is neither sufficient to expressively elicit an antiproliferative response nor to induce the hormone transcriptional signaling pathway in breast cancer specimens.
article 32 Citação(ões) na Scopus Transcriptional profile of fibroblasts obtained from the primary site, lymph node and bone marrow of breast cancer patients(2014) VALLE, Paulo Roberto Del; MILANI, Cintia; BRENTANI, Maria Mitzi; KATAYAMA, Maria Lucia Hirata; LYRA, Eduardo Carneiro de; CARRARO, Dirce Maria; BRENTANI, Helena; PUGA, Renato; LIMA, Leandro A.; ROZENCHAN, Patricia Bortman; NUNES, Barbara dos Santos; GOES, Joao Carlos Guedes Sampaio; FOLGUEIRA, Maria Aparecida Azevedo KoikeCancer-associated fibroblasts (CAF) influence tumor development at primary as well as in metastatic sites, but there have been no direct comparisons of the transcriptional profiles of stromal cells from different tumor sites. In this study, we used customized cDNA microarrays to compare the gene expression profile of stromal cells from primary tumor (CAF, n = 4), lymph node metastasis (N+, n = 3) and bone marrow (BM, n = 4) obtained from breast cancer patients. Biological validation was done in another 16 samples by RT-qPCR. Differences between CAF vs N+, CAF vs BM and N+ vs BM were represented by 20, 235 and 245 genes, respectively (SAM test, FDR < 0.01). Functional analysis revealed that genes related to development and morphogenesis were overrepresented. In a biological validation set, NOTCH2 was confirmed to be more expressed in N+ (vs CAF) and ADCY2, HECTD1, HNMT, LOX, MACF1, SLC1A3 and USP16 more expressed in BM (vs CAF). Only small differences were observed in the transcriptional profiles of fibroblasts from the primary tumor and lymph node of breast cancer patients, whereas greater differences were observed between bone marrow stromal cells and the other two sites. These differences may reflect the activities of distinct differentiation programs.- TGF-beta(1) expression in wound healing is acutely affected by experimental malnutrition and early enteral feeding(2014) ALVES, Claudia Cristina; TORRINHAS, Raquel Susana; GIORGI, Ricardo; BRENTANI, Maria Mitzi; LOGULLO, Angela Flavia; WAITZBERG, Dan LinetzkyMalnutrition is associated with the delay or failure of healing. We assessed the effect of experimental malnutrition and early enteral feeding with standard diet or diet supplemented with arginine and antioxidants on the levels of mRNA encoding growth factors in acute, open wound healing. Standardised cutaneous dorsal wounds and gastrostomies for enteral feeding were created in malnourished (M, n = 27) and eutrophic control (E, n = 30) Lewis male adult rats. Both M and E rats received isocaloric and isonitrogenous regimens with oral chow and saline (C), standard (S) or supplemented (A) enteral diets. On post-trauma day 7, mRNA levels of growth factor genes were analysed in wound granulation tissue by reverse transcription polymerase chain reaction (RT-PCR). M(C) rats had significantly lower transforming growth factor (TGF-(1)) mRNA levels than E(C) rats (258 +/- 083 versus 353 +/- 057, P < 001) and in comparison with M(S) and M(A) rats (466 +/- 249 and 461 +/- 211, respectively; P < 005). VEGF and KGF-7 mRNA levels were lower in M(A) rats than in E(A) rats (074 +/- 016 versus 125 +/- 066; and 107 +/- 045 versus 179 +/- 089, respectively; P 004), but did not differ from levels in E(C) and M(C) animals. In experimental open acute wound healing, previous malnutrition decreased local mRNA levels of TGF-(1) genes, which was minimised by early enteral feeding with standard or supplemented diets.
conferenceObject Association of stromal cell gene expression with response to neoadjuvant chemotherapy in locally advanced breast cancer(2014) VIEIRA, Rene Aloisio da Costa; MATTHES, Angelo Gustavo Zucca; KATAYAMA, Maria Lucia Hirata; ANDRADE, Victor Piana; ROELA, Rosimeire Aparecida; LIMA, Luiz Guilherme C. A.; MAISTRO, Simone; BRENTANI, Maria Mitzi; FOLGUEIRA, Maria A. A. Koike- Gene Expression Profiles in Breast Cancer to Identify Estrogen Receptor Target Genes(2014) NAGAI, M. A.; BRENTANI, M. M.The estrogens play important role in the homeostatic maintenance of several target tissues including those in the mammary gland, uterus, bone, cardiovascular system, and brain. Most of estrogen's action is thought to be mediated through its nuclear estrogen receptors, ERα and ERβ, which are members of the nuclear receptor superfamily that act as ligand-induced transcription factors. Acting via its receptors, estrogen also plays an essential role in the development and progression of human breast cancer. The ER and progesterone receptor (PR), which is regulated by estrogen via ER, have been used as prognostic markers in the clinical management of breast cancer patients. However, the prognosis of a patient with ER+/PR+ breast cancer can be highly variable and a significant proportion of hormone receptor positive breast cancers do not respond to endocrine therapy. The identification of estrogen receptor target genes may improve our understanding of the role played by estrogens in breast cancer making it possible to better tailor hormone treatments and improve a patient's response to hormonal therapy. In this review, we explore the literature for data regarding the identification of estrogen receptor-regulated genes in breast cancer cell lines and breast tumor biopsies using high throughput technologies such as serial analysis of gene expression (SAGE) and cDNA microarrays. © 2014 Bentham Science Publishers Ltd. Published by Elsevier Inc. All rights reserved.
- RHOA, RAC1 and PAK1 evaluation in paired stromal fibroblasts of breast cancer primary and of lymph node metastasis: Importance of these biomarkers in lymph node invasion(2014) ROZENCHAN, Patricia Bortman; MUNDIM, Fiorita G.; ROELA, Rosimeire A.; KATAYAMA, Maria L.; PASINI, Fatima S.; BRENTANI, Helena; LYRA, Eduardo C.; FOLGUEIRA, Maria A. A. K.; BRENTANI, Maria M.