JOSE CARLOS MARQUES DE FARIA
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Cirurgia, Faculdade de Medicina - Docente
LIM/04 - Laboratório de Microcirurgia, Hospital das Clínicas, Faculdade de Medicina
LIM/04 - Laboratório de Microcirurgia, Hospital das Clínicas, Faculdade de Medicina
2 resultados
Resultados de Busca
Agora exibindo 1 - 2 de 2
- Evaluation of the Use of Nerve Allograft Preserved in Glycerol(2021) NAKAMOTO, Joao Carlos; WATAYA, Erick Yoshio; NAKAMOTO, Hugo Alberto; SANTOS, Gustavo Bispo; RIBARIC, Ivan; HERRERA, Ana K. A.; FARIA, Jose C. M.Background: We aimed to evaluate the use of nerve allograft preserved in glycerol. We compared the efficiency of glycerol-preserved allografts with autogenous nerve grafting, cryopreserved grafts, and detergent-processed grafts in the axonal regeneration. Secondarily, we evaluated the effectiveness of each preservation method in maintaining the extracellular matrix free of cellular components. Methods: This was a prospective experimental, longitudinal, unblinded, nonrandomized, controlled animal model study. Three different allograft preservation techniques for the repair of sciatic nerve injuries were compared, including cold preservation, glycerol preservation, and detergent preservation. Functional assessment was performed, and histomorphometric analyses were further performed, which enabled the allograft structure evaluation and an estimation of the nerve regeneration efficacy based on the myelinated axons count and on their diameters. Results: After the 14(th) week, all groups were already balanced and similar (P = 0.265): all groups present near-zero SFIs, thus confirming their efficiency in promoting nerve regeneration. In the histomorphometric evaluations, all groups were equivalent, presenting a similar efficiency in nerve regeneration (P = 0.716 and P = 0.577, respectively). Similarly, histomorphometric evaluations showed a reduction in the number of axons and in their diameters, but none of them effectively eliminated all cellular debris. Comparing the groups with each other, the groups preserved in glycerol and detergent solution were similar, both presenting better results than the cooled group. Conclusion: By evaluating the presence of cell debris after the treatment using glycerol, it was found to be similar to the treatment using detergent and significantly better than the cold-preservation treatment.
- Forehead reconstruction using supraclavicular flap with microsurgical technique: Free flap and a pedicle supercharged flap(2017) ALVES, H. R. N.; FARIA, J. C. M. de; BUSNARDO, F.; CERNEA, C.; RANGEL, T.; GEMPERLI, R.Large and deep defects in foreheads are best repaired with flaps. In these types of cases, reconstructions are made through the transfer of distant flaps, due to the small amount of available local tissue. The supraclavicular island flap (SCIF) has been extensive used for head and neck reconstructions during the last two decades. However, the flap extension is limited to the reconstruction of the superior third of the face, which may increase the risk of distal flap necrosis. The authors describe two cases of forehead reconstruction with the SCIF using a microsurgical technique. © 2017 The Author(s)