ANNA LAURA VIACAVA AMERICO
Projetos de Pesquisa
Unidades Organizacionais
LIM/13 - Laboratório de Genética e Cardiologia Molecular, Hospital das Clínicas, Faculdade de Medicina
16 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 16
- Aerobic Physical Exercise Improves Exercise Tolerance and Fasting Glycemia Independent of Body Weight Change in Obese Females(2021) BOSCHETTI, Daniela; MULLER, Cynthia R.; AMERICO, Anna Laura V.; VECCHIATTO, Bruno; MARTUCCI, Luiz Felipe; PEREIRA, Renata O.; OLIVEIRA, Claudia P.; FIORINO, Patricia; EVANGELISTA, Fabiana S.; AZEVEDO-MARTINS, Anna KareninaObesity is associated with increased risk of several chronic diseases and the loss of disease-free years, which has increased the focus of much research for the discovery of therapy to combat it. Under healthy conditions, women tend to store more fat in subcutaneous deposits. However, this sexual dimorphism tends to be lost in the presence of comorbidities, such as type 2 diabetes mellitus (T2DM). Aerobic physical exercise (APE) has been applied in the management of obesity, however, is still necessary to better understand the effects of APE in obese female. Thus, we investigated the effect of APE on body weight, adiposity, exercise tolerance and glucose metabolism in female ob/ob mice. Eight-weeks-old female wild-type C57BL/6J and leptin-deficient ob/ob mice (Lep(ob)) were distributed into three groups: wild-type sedentary group (Wt; n = 6), leptin-deficient sedentary group (Lep(ob)S; n = 5) and leptin-deficient trained group (Lep(ob)T; n = 8). The Lep(ob)T mice were subjected to 8 weeks of aerobic physical exercise (APE) at 60% of the maximum velocity achieved in the running capacity test. The APE had no effect in attenuating body weight gain, and did not reduce subcutaneous and retroperitoneal white adipose tissue (SC-WAT and RP-WAT, respectively) and interscapular brown adipose tissue (iBAT) weights. The APE neither improved glucose intolerance nor insulin resistance in the Lep(ob)T group. Also, the APE did not reduce the diameter or the area of RP-WAT adipocytes, but the APE reduced the diameter and the area of SC-WAT adipocytes, which was associated with lower fasting glycemia and islet/pancreas area ratio in the Lep(ob)T group. In addition, the APE increased exercise tolerance and this response was also associated with lower fasting glycemia in the Lep(ob)T group. In conclusion, starting APE at a later age with a more severe degree of obesity did not attenuate the excessive body weight gain, however the APE promoted benefits that can improve the female health, and for this reason it should be recommended as a non-pharmacological therapy for obesity.
- Aerobic exercise training prevents kidney lipid deposition in mice fed a cafeteria diet(2018) MULLER, C. R.; V, A. L. Americo; FIORINO, P.; EVANGELISTA, F. S.Aim: The objective of this study was to investigate the potential of aerobic exercise training (AET) to prevent kidney lipid accumulation and the contribution of renal metabolism to mediate this response. Main methods: Male C57BL/6J mice were assigned into groups CHOW-SED (chow diet, sedentary; n = 13), CHOW-TR (chow diet, trained; n = 13), CAF-SED (cafeteria diet, sedentary; n = 13) and CAF-TR (cafeteria diet, trained; n = 13). AET consisted in running sessions of 60 min at 60% of maximal speed conducted five days per week for eight weeks. Key findings: AET prevented weight gain in both trained groups. Food intake was not different among groups, however water intake, urine output, urine potassium and osmolarity were reduced in CAF-SED and CAF-TR groups. Kidney lipid deposition increased in CAF-SED (4.12 +/- 0.5%/area) compared with CHOW-SED (1.7 +/- 0.54%/area), and the AET prevented this increase in the CAF-TR group (2.1 +/- 0.5%/area). The Bowman's capsule area decreased in CAF-SED and CAF-TR groups while the Bowman' space reduced in CAF-SED compared to CHOW-SED group, which was prevented by AET in the CAF-TF group. We observed a 27% increase in the p-AMPK expression in CAF-TR compared to CHOW-SED group without differences in the SIRT-1, PGC1-alpha, ACC and p-ACC. beta-HAD activity increased in CAF-SED (43.9 +/- 4.57 nmol.min(-1).ug(-1)) and CAF-TR (44.7 +/- 2.6 nmol.min(-1).ug(-1)) groups compared to CHOW-SED (35.1 +/- 2.9 nmol.min(-1).ug(-1)) e CHOW-TR (36.6 +/- 2.7 nmol.min(-1).ug(-)1). Significance: AET prevented kidney lipid accumulation induced by cafeteria diet and this response was not associated with changes in the renal metabolic activity that favors lipid oxidation.
- Effects of Aerobic Exercise Protocol on Genes Related to Insulin Resistance and Inflammation in the Pancreas of ob/ob Mice with NAFLD(2020) SILVA, Lucas Lucena Simoes e; FERNANDES, Matheus Santos de Sousa; KUBRUSLY, Marcia Saldanha; MULLER, Cynthia Rodrigues; AMERICO, Anna Laura Viacava; STEFANO, Jose Tadeu; EVANGELISTA, Fabiana Sant'Anna; OLIVEIRA, Claudia Pinto; JUKEMURA, JosePurpose: To evaluate the effect of 8 weeks of aerobic training on insulin resistance and inflammatory response in obese mice (ob/ob) with NAFLD. Materials and Methods: Male ob/ob mice were randomly divided into sedentary (n=7) and trained (n=7) groups. Aerobic training consisted of 5 weekly sessions, 60 min per session at 60% of the maximum speed of the running test. Hepatic and pancreatic samples were collected to evaluate histological features and gene expression associated with insulin resistance and inflammatory response after 8-week experiment protocol. RNA was performed by TRIzol (R). PCR experiments were performed using the Rotor-Gene RG-3000. Parametric data were assessed by t-test, one-way ANOVA and Bonferroni test for multiple comparisons. Non-parametric data were assessed by the Mann-Whitney tests with Dunn's post-test of multiple comparisons. Histological analysis was assessed by chi-square test with Fisher's exact test. Significant variables were considered when p<0.05. All the analyses were performed by GraphPad Prism V6.0 software (GraphPad Software Inc.). Results: Reductions in bodyweight (p = 0.008), weight evolution (p = 0.03), food intake (p <0.0001) and fat content were observed in trained group. Moreover, the trained group showed better results in peak velocity (p=0.03) physical effort tolerance (p=0.006) and distance (p=0.01). Gene expression showed differences in IL-10 (p=0.03) and GLUT-2 (p=0.03) in hepatic analysis, between groups. Pancreatic gene expression showed difference between groups in IRS-2 (p=0.004), GLUT-2 (p=0.03) and IL-10 (p=0.008) analysis. Also, the trained group showed lower values for interlobular fat and inflammatory infiltrate in histological analysis when compared to sedentary animals. Conclusion: An 8-week physical training protocol was able to attenuate bodyweight gain, food intake and generate positive effects on gene expression related to insulin resistance and inflammation in both liver and pancreas of ob/ob mice.
- Oxidative phenotype induced by aerobic physical training prevents the obesity-linked insulin resistance without changes in gastrocnemius muscle ACE2-Angiotensin(1-7)-Mas axis(2021) VECCHIATTO, Bruno; SILVA, Rafael C. da; HIGA, Talita S.; MULLER, Cynthia R.; V, Anna Laura Americo; FORTUNATO-LIMA, Vanessa C.; FERREIRA, Marilia M.; MARTUCCI, Luiz Felipe; FONSECA-ALANIZ, Miriam H.; EVANGELISTA, Fabiana S.Background We investigate the effect of aerobic physical training (APT) on muscle morphofunctional markers and Angiotensin Converting Enzyme 2/Angiotensin 1-7/Mas receptor (ACE2/Ang 1-7/Mas) axis in an obesity-linked insulin resistance (IR) animal model induced by cafeteria diet (CAF). Methods Male C57BL/6J mice were assigned into groups CHOW-SED (chow diet, sedentary; n = 10), CHOW-TR (chow diet, trained; n = 10), CAF-SED (n = 10) and CAF-TR (n = 10). APT consisted in running sessions of 60 min at 60% of maximal speed, 5 days per week for 8 weeks. Results Trained groups had lower body weight and adiposity compared with sedentary groups. CAF-TR improved the glucose and insulin tolerance tests compared with CAF-SED group (AUC = 28.896 +/- 1589 vs. 35.200 +/- 1076 mg dL(-1) 120 min(-1); kITT = 4.1 +/- 0.27 vs. 2.5 +/- 0.28% min(-1), respectively). CHOW-TR and CAF-TR groups increased exercise tolerance, running intensity at which VO2 max was reached, the expression of p-AMPK, p-ACC and PGC1-alpha proteins compared with CHOW-SED and CAF-SED. Mithocondrial protein expression of Mfn1, Mfn2 and Drp1 did not change. Lipid deposition reduced in CAF-TR compared with CAF-SED group (3.71 vs. 5.53%/area), but fiber typing, glycogen content, ACE2 activity, Ang 1-7 concentration and Mas receptor expression did not change. Conclusions The APT prevents obesity-linked IR by modifying the skeletal muscle phenotype to one more oxidative independent of changes in the muscle ACE2/Ang 1-7/Mas axis.
conferenceObject Differentiated contribution of fat pads to the prevention of insulin resistance through aerobic exercise training(2017) AMERICO, Anna Laura Viacava; MULLER, Cynthia Rodrigues; FONSECA-ALANIZ, Miriam Helena; EVANGELISTA, Fabiana Sant'AnnaconferenceObject Exercise training prevents hypertension, dyslipidemia and renal lipid deposition in young rats fed a high-fat diet(2015) MULLER, Cynthia; AMERICO, Anna Laura; NETO, Giuseppe; EVANGELISTA, Fabiana; FARAH, Vera; FIORINO, Patricia- Potential of Aerobic Physical Training to Minimize the Effects of Leptin Deficiency in Female Mice(2020) AZEVEDO-MARTINS, Anna; BOSCHETTI, Daniela; MULLER, Cynthia; AMERICO, Anna; VECCHIATTO, Bruno; MARTUCCI, Luiz; PEREIRA, Renata; OLIVEIRA, Claudia; EVANGELISTA, Fabiana
conferenceObject Aerobic exercise training prevents renal damage in insulin resistance mice(2017) MULLER, Cynthia Rodrigues; AMERICO, Anna Laura Viacava; FIORINO, Patricia; EVANGELISTA, Fabiana Sant'Anna- Exposure to high-fat diet since post-weaning induces cardiometabolic damage in adult rats(2016) FIORINO, P.; AMERICO, A. L. V.; MULLER, C. R.; EVANGELISTA, F. S.; SANTOS, F.; LEITE, A. P. O.; FARAH, V.Aims: This study sought to investigate the metabolic, hemodynamic and autonomic responses in adult rats exposed to high-fat diet since post-weaning. Main methods: Young male Wistar rats were assigned into groups fed with standard normal diet (3% lipids; ND, n=8) or high-fat diet (30% lipids; HD, n=8) during 8 weeks. Body composition, food intake, serum triglycerides, total cholesterol, insulin, leptin and adiponectin concentrations were determined. Hemodynamic and autonomic evaluations were performed. Renin angiotensin system and nitric oxide were also studied by pharmacological blockades. Key findings: HD group showed no difference in body weight, total cholesterol, food intake in calories and insulin concentration, but visceral fat pads weight, triglycerides and leptin were higher in HD group. Moreover, HD group decreased adiponectin level, increased 12% of mean arterial pressure (MAP) and 6% of heart rate compared with ND group. Spectral analyses showed an increase in cardiovascular sympathetic modulation in HD compared with ND group. Depressor responses after losartan were higher in HD compared with ND group: -9 +/- 0.7 vs.-3 +/- 1.6 mmHg. Pressor responses after L-NAME were higher in HD compared with ND: 45 +/- 8 vs. 32 +/- 5 mmHg. Significance: High-fat diet consumption during early period of life can increase WAT mass and MAP. These alterations may be mediated by an augment in sympathetic activity associated with higher leptin and lower adiponectin levels. These cardiometabolic damages can lead to the development of hypertension and increase cardiovascular risk in adulthood.
- Disruption of beta3 adrenergic receptor increases susceptibility to DIO in mouse(2016) PREITE, Nailliw Z.; NASCIMENTO, Bruna P. P. do; MULLER, Cynthia R.; AMERICO, Anna Laura V.; HIGA, Talita S.; EVANGELISTA, Fabiana S.; LANCELLOTTI, Carmen L.; HENRIQUES, Felipe dos Santos; BATISTA JR., Miguel Luiz; BIANCO, Antonio C.; RIBEIRO, Miriam O.The brown adipose tissue (BAT) mediates adaptive changes in metabolic rate by responding to the sympathetic nervous system through beta-adrenergic receptors (AR). Here, we wished to define the role played by the AR beta(3) isoform in this process. This study focused on the AR beta(3) knockout mice (AR beta 3KO), including responsiveness to cold exposure, diet-induced obesity, intolerance to glucose, dyslipidaemia and lipolysis in white adipose tissue (WAT). AR beta 3KO mice defend core temperature during cold exposure (4 degrees C for 5h), with faster BAT thermal response to norepinephrine (NE) infusion when compared with wild-type (WT) mice. Despite normal BAT thermogenesis, AR beta 3KO mice kept on a high-fat diet (HFD; 40% fat) for 8 weeks exhibited greater susceptibility to diet-induced obesity, markedly increased epididymal adipocyte area with clear signs of inflammation. The HFD-induced glucose intolerance was similar in both groups but serum hypertriglyceridemia and hypercholesterolemia were less intense in AR beta 3KO animals when compared with WT controls. Isoproterenol-induced lipolysis in isolated white adipocytes as assessed by glycerol release was significantly impaired in AR beta 3KO animals despite normal expression of key proteins involved in lipid metabolism. In conclusion, AR beta(3) inactivation does not affect BAT thermogenesis but increases susceptibility to diet-induced obesity by dampening WAT lipolytic response to adrenergic stimulation.