JOSE ERNESTO VIDAL BERMUDEZ
Projetos de Pesquisa
Unidades Organizacionais
P ICHC, Hospital das Clínicas, Faculdade de Medicina - Médico
LIM/49 - Laboratório de Protozoologia, Hospital das Clínicas, Faculdade de Medicina - Líder
LIM/49 - Laboratório de Protozoologia, Hospital das Clínicas, Faculdade de Medicina - Líder
8 resultados
Resultados de Busca
Agora exibindo 1 - 8 de 8
- BRAIN ABSCESS DUE TO Staphylococcus aureus OF CRYPTOGENIC SOURCE IN AN HIV-1 INFECTED PATIENT IN USE OF ANTIRETROVIRAL THERAPY(2016) OLIVEIRA, Anna Paula Romero de; PAPPALARDO, Mara Cristina; DANTAS, Daniel; LINS, Diogo; VIDAL, Jose ErnestoThe spectrum of neurological complications associated with human immunodeficiency virus type 1 (HIV-1) infection is broad. The most frequent etiologies include primary diseases (caused by HIV itself) or secondary diseases (opportunistic infections or neoplasms). Despite these conditions, HIV-infected patients are susceptible to other infections observed in patients without HIV infection. Here we report a rare case of a brain abscess caused by Staphylococcus aureus in an HIV-infected patient. After drainage of the abscess and treatment with oxacilin, the patient had a favorable outcome. This case reinforces the importance of a timely neurosurgical procedure that supported adequate management of an unusual cause of expansive brain lesions in HIV-1 infected patients.
- Asymptomatic cryptococcal antigen prevalence detected by lateral flow assay in hospitalised HIV-infected patients in Sao Paulo, Brazil(2016) VIDAL, Jose E.; TONIOLO, Carolina; PAULINO, Adriana; COLOMBO, Arnaldo; MARTINS, Marilena dos Anjos; MEIRA, Cristina da Silva; PEREIRA-CHIOCCOLA, Vera Lucia; FIGUEIREDO-MELLO, Claudia; BARROS, Tiago; DUARTE, Jequelie; FONSECA, Fernanda; CUNHA, Mirella Alves; MENDES, Clara; RIBERO, Taiana; LAZERA, Marcia dos Santos; RAJASINGHAM, Radha; BOULWARE, David R.objective To determine the prevalence of asymptomatic cryptococcal antigen (CRAG) using lateral flow assay (LFA) in hospitalised HIV-infected patients with CD4 counts < 200 cells/mu l. methods Hospitalised HIV-infected patients were prospectively recruited at Instituto de Infectologia Emilio Ribas, a tertiary referral hospital to HIV-infected patients serving the Sao Paulo State, Brazil. All patients were > 18 years old without prior cryptococcal meningitis, without clinical suspicion of cryptococcal meningitis, regardless of antiretroviral (ART) status, and with CD4 counts < 200 cells/mu l. Serum CRAG was tested by LFA in all patients, and whole blood CRAG was tested by LFA in positive cases. results We enrolled 163 participants of whom 61% were men. The duration of HIV diagnosis was a median of 8 (range, 1-29) years. 26% were antiretroviral (ART)-naive, and 74% were ARTexperienced. The median CD4 cell count was 25 (range, 1-192) cells/mu l. Five patients (3.1%; 95% CI, 1.0-7.0%) were asymptomatic CRAG-positive. Positive results cases were cross-verified by performing LFA in whole blood. conclusions 3.1% of HIV-infected inpatients with CD4 < 200 cells/ll without symptomatic meningitis had cryptococcal antigenemia in Sao Paulo, suggesting that routine CRAG screening may be beneficial in similar settings in South America. Our study reveals another targeted population for CRAG screening: hospitalised HIV-infected patients with CD4 < 200 cells/mu l, regardless of ART status. Whole blood CRAG LFA screening seems to be a simple strategy to prevention of symptomatic meningitis.
- Genotyping of Toxoplasma gondii: DNA extraction from formalin-fixed paraffin-embedded autopsy tissues from AIDS patients who died by severe disseminated toxoplasmosis(2016) SILVA, Inara Bastos da; BATISTA, Tatiana Pimental de Andrade; MARTINES, Roosecelis Brasil; KANAMURA, Cristina Takami; FERREIRA, Isabelle Martins Ribeiro; VIDAL, Jose Ernesto; PEREIRA-CHIOCCOLA, Vera LuciaThis study investigated the genetic features of Toxoplasma gondii isolated directly in autopsies of HIV-infected patients who died with severe disseminated toxoplasmosis. This retrospective analysis was conducted in a cohort of 15 HIV-infected patients with clinical and laboratory data. They had previous cerebral toxoplasmosis at least 6 months before the disseminated toxoplasmosis episode. The hypothesis was that they were infected with highly virulent parasites due to the condition in which they died. T. gondii genotyping was done directly in DNA extracted from 30 autopsy brain and lung samples (2 per patient) and mutilocus PCR-RFLP genotyping was done using 12 molecular markers. The 30 clinical samples were genotyped successfully in 8 or more loci and six suggestive genotypes were identified. One of them was Toxo DB #11, previously identified in different domestic animals and virulent in experimental animals. The other five suggestive genotypes identified in 14 patients were not described. TgHuDis1 was the most frequent and was determined in 8 patients. TgHuDis3 and TgHuDis5 were identified in two patients each. TgHuDis2 and TgHuDis4 have been identified in one patient each. These suggestive genotypes could be considered as virulent, since they caused severe tissue damage and had similar characteristics as Toxo # DB 11.
bookPart Infecção pelo Vírus da Imunodeficiência Humana (HIV) e Síndrome da Imunodeficiência Adquirida (Aids)(2016) SEGURADO, Aluísio Augusto Cotrim; FREITAS, Angela Carvalho; ATOMIYA, Angela Naomi; WüNSCH, Celia Torrens; NOVAES, Christina Terra Gallafrio; HIGASHINO, Hermes Ryoiti; BERMUDEZ, Jose Ernesto Vidal; CHAVES NETTO, Lucas; VICENTINE, Margarete Paganotti; BOULOS, Maria Ivete Castro; LOPES, Max Igor Banks Ferreira; VASCONCELOS, Ricardo de Paula; SANTOS, Sigrid de Sousa dos; MELLO, Valéria Antakly de; AVELINO-SILVA, Vivian Helena IidabookPart PRINCIPAIS DOENÇAS NEUROLÓGICAS OPORTUNISTAS EM PACIENTES INFECTADOS(2016) VIDAL, José Ernesto; BOSZCZOWSKI, Ícaro; OLIVEIRA, Augusto César Penalva de- Meningococcal meningitis: clinical and laboratorial characteristics, fatality rate and variables associated with in-hospital mortality(2016) STRELOW, Vanessa L.; MIRANDA, Erique Jose F. Peixoto de; KOLBE, Karin R.; FRAMIL, Juliana V. Souza; OLIVEIRA, Augusto Penalva de; VIDAL, Jose E.Meningococcal meningitis is a public health problem. The aim of this study was to describe the clinical characteristics of patients with meningococcal meningitis, and to identify associated factors with mortality. This was a retrospective study, between 2006 and 2011, at a referral center in Sao Paulo, Brazil. Logistic regression analysis was used to identify factors associated with mortality. We included 316 patients. The median age was 16 years (IQR: 7-27) and 60% were male. The clinical triad: fever, headache and neck stiffness was observed in 89% of the patients. The cerebrospinal triad: pleocytosis, elevated protein levels and low glucose levels was present in 79% of patients. Factors associated with mortality in the multivariate model were age above 50 years, seizures, tachycardia, hypotension and neck stiffness. The classic clinical and laboratory triads of meningococcal meningitis were variable. The fatality rate was low. Age, seizures and shock signs were independently associated with mortality.
bookPart MENINGITES BACTERIANAS PÓS-OPERATÓRIAS EM NEUROCIRURGIA(2016) BOSZCZOWSKI, Ícaro; VIDAL, José ErnestobookPart Meningites, meningoencefalites e encefalites(2016) FRAMIL, Juliana Valéria de Souza; VIDAL, José E.