DARLAN DA SILVA CANDIDO
Projetos de Pesquisa
Unidades Organizacionais
LIM/19 - Laboratório de Histocompatibilidade e Imunidade Celular, Hospital das Clínicas, Faculdade de Medicina
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conferenceObject CIRCULATING MIRNAS PROFILE AS POTENTIAL SIGNATURE OF BENZNIDAZOLE TREATMENT TOXICITY IN CHAGAS PATIENTS(2017) CANDIDO, Darlan da Silva; CUNHA-NETO, Edecio; RIGAUD, Vagner O.; OLIVEIRA, Lea C. de; MOREIRA, Carlos Henrique V.; JUNIOR, Nelson G.; SOUZA, Marcela de; SABINO, Ester C.; FERREIRA, Ludmila R.- Satisfaction of Chagas disease patients attended at a pharmaceutical care service in the State of Ceara, Brazil(2018) COSTA, Alanna Carla da; CANDIDO, Darlan da Silva; FIDALGO, Arduina Sofia Ortet de Barros Vasconcelos; SILVA FILHO, Jose Damiao da; VIANA, Carlos Eduardo Menezes; LIMA, Monise Anne; ANDRADE, Monica Coelho; OLIVEIRA, Maria de FatimaIn 2005, a pharmaceutical care service was created in the State of Ceara to provide pharmacotherapeutic follow-up for individuals infected with Trypanosoma cruzi (Chagas Disease). After 10 years of operation, an evaluation was conducted to assess the degree of satisfaction of patients treated under the service. This prospective study used a questionnaire comprising the following sections: socioeconomic data; infrastructure, facilities and operations; pharmaceutical care; and importance of the service. Seventy patients of both sexes and over 18 years of age were interviewed between August 2014 and May 2015. As for infrastructure, location and operation, the average grades show a high level of patient satisfaction. Regarding pharmaceutical care, most patients reported being satisfied and considered ""being well treated"" to be the most important aspect during treatment. In addition, all patients (100%) rated the service as very important and would recommend it to other individuals. Overall, the study showed a high level of patient satisfaction with the service. There is, however, still much to work to be done on this service in order to promote greater access and qualified care to fully achieve a humanized model focused on patient needs.
- Myocardial Infarction-Associated Transcript, a Long Noncoding RNA, Is Overexpressed During Dilated Cardiomyopathy Due to Chronic Chagas Disease(2016) FRADE, Amanda Farage; LAUGIER, Laurie; FERREIRA, Ludmila Rodrigues Pinto; BARON, Monique Andrade; BENVENUTI, Luiz Alberto; TEIXEIRA, Priscila Camillo; NAVARRO, Isabela Cunha; CABANTOUS, Sandrine; FERREIRA, Frederico Moraes; CANDIDO, Darlan da Silva; GAIOTTO, Fabio Antonio; BACAL, Fernando; POMERANTZEFF, Pablo; SANTOS, Ronaldo Honorato Barros; KALIL, Jorge; CUNHA-NETO, Edecio; CHEVILLARD, ChristopheLong noncoding RNAs (lncRNAs) modulate gene expression at the epigenetic, transcriptional, and posttranscriptional levels. Dysregulation of the lncRNA known as myocardial infarction-associated transcript (MIAT) has been associated with myocardial infarction. Chagas disease causes a severe inflammatory dilated chronic cardiomyopathy (CCC). We investigated the role of MIAT in CCC. A whole-transcriptome analysis of heart biopsy specimens and formalin-fixed, paraffin-embedded samples revealed that MIAT was overexpressed in patients with CCC, compared with subjects with noninflammatory dilated cardiomyopathy and controls. These results were confirmed in a mouse model. Results suggest that MIAT is a specific biomarker of CCC.
- Pharmacotherapeutic follow-up of patients with Chagas disease using in use of benznidazole: drug-related problems and pharmaceutical interventions(2017) CORREIA, Joao Paulo Ramalho; COSTA, Alanna Carla da; ROCHA, Eduardo Arrais; QUIDUTE, Ana Rosa Pinto; CANDIDO, Darlan da Silva; PONCIANO, Angela Maria de Souza; FONTELES, Marta Maria de Franca; OLIVEIRA, Maria de FatimaIntroduction: Benznidazole (BNZ) is a drug available for the etiological treatment of Chagas disease. However, this drug is toxic and has a limited effectiveness on the chronic phase of this disease, often leading to poor treatment adherence. Methods: This is a descriptive and exploratory study conducted at the Pharmaceutical Care Service for Chagas disease patients of the Federal University of Ceara. Drug-related problems (DRPs) and pharmaceutical interventions (PIs) were classified according to the Second Consensus of Granada. Results: The average age of patients with Chagas disease was 62 years, with the majority residing in the Ceara countryside (86.7%), and having low education levels (63.3% with elementary school education). Regarding family income, most patients belonged to a household that earned <= 1-2 times the minimum wage per month. Approximately 73% of these patients complied with the BNZ treatment, and nearly 7% underwent therapy interruption after medical evaluation. A total of 189 DRPs were identified, of which 51.9% (n=98) were classified as potential, and 48.1% (n= 91) as actual. The most frequent DRPs were related to safety (qualitative safety; n=70; 37%), necessity (non-adherence; n=52; 27.5%), and effectiveness (qualitative effectiveness/non-optimal drug selection; n=45; 23.8%). Among the 216 PIs conducted, the majority were related to patient education (n=168; 77.8%) and pharmacological strategy (n=42; 19.4%). Conclusions: This study indicates the need for pharmacotherapeutic monitoring in patients with Chagas because of the high number of therapeutic interventions, DRPs (approximately 3 DRPs/patient), BNZ adherence, and polypharmacy.
- Integration of miRNA and gene expression profiles suggest a role for miRNAs in the pathobiological processes of acute Trypanosoma cruzi infection(2017) FERREIRA, Ludmila Rodrigues Pinto; FERREIRA, Frederico Moraes; LAUGIER, Laurie; CABANTOUS, Sandrine; NAVARRO, Isabela Cunha; CANDIDO, Darlan da Silva; RIGAUD, Vagner Carvalho; REAL, Juliana Monte; PEREIRA, Glaucia Vilar; PEREIRA, Isabela Resende; RUIVO, Leonardo; PANDEY, Ramendra Pati; SAVOIA, Marilda; KALIL, Jorge; LANNES-VIEIRA, Joseli; NAKAYA, Helder; CHEVILLARD, Christophe; CUNHA-NETO, EdecioChagas disease, caused by the parasite Trypanosoma cruzi, is endemic in Latin America. Its acute phase is associated with high parasitism, myocarditis and profound myocardial gene expression changes. A chronic phase ensues where 30% develop severe heart lesions. Mouse models of T. cruzi infection have been used to study heart damage in Chagas disease. The aim of this study was to provide an interactome between miRNAs and their targetome in Chagas heart disease by integrating gene and microRNA expression profiling data from hearts of T. cruzi infected mice. Gene expression profiling revealed enrichment in biological processes and pathways associated with immune response and metabolism. Pathways, functional and upstream regulator analysis of the intersections between predicted targets of differentially expressed microRNAs and differentially expressed mRNAs revealed enrichment in biological processes and pathways such as IFN gamma, TNF alpha, NF-kappa B signaling signatures, CTL-mediated apoptosis, mitochondrial dysfunction, and Nrf2-modulated antioxidative responses. We also observed enrichment in other key heart disease-related processes like myocarditis, fibrosis, hypertrophy and arrhythmia. Our correlation study suggests that miRNAs may be implicated in the pathophysiological processes taking place the hearts of acutely T. cruzi-infected mice.