Cell death mechanisms in Leishmania amazonensis triggered by methylene blue-mediated antiparasitic photodynamic therapy

Página do item simplificado
dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorAURELIANO, Debora P.
dc.contributor.authorLINDOSO, Jose Angelo Lauletta
dc.contributor.authorSOARES, Sandra Regina de Castro
dc.contributor.authorTAKAKURA, Cleusa Fumika Hirata
dc.contributor.authorPEREIRA, Thiago Martini
dc.contributor.authorRIBEIRO, Martha Simoes
dc.date.accessioned2018-11-21T17:06:23Z
dc.date.available2018-11-21T17:06:23Z
dc.date.issued2018
dc.description.abstractAntiparasitic photodynamic therapy (ApPDT) is an emerging approach to manage cutaneous leishmaniasis (CL) since no side effects, contraindications and parasite resistance have been reported. In addition, methylene blue (MB) is a suitable photosensitizer to mediate ApPDT on CL. In this study we aimed to look for the best parameters to eradicate Leishmania amazonensis and investigated the cell death pathways involved in MB-mediated ApPDT. MB uptake by parasites was determined using different MB concentrations (50, 100, 250 and 500 mu M) and incubation times (10, 30 and 60 min). L. amazonensis promastigotes were cultured and submitted to ApPDT using different concentrations of MB (50, 100 and 250 mu M) combined to a red LED emitting at 645 +/- 10 nm. The pre-irradiation time was 10 min. Two optical powers (100 mW and 250 mW) were tested and cells were exposed to 60 and 300 s of MB-mediated ApPDT delivering energies of 6, 15, 30 and 75 J and fluences of 21.2, 53.1, 106.2 and 265.4 J/cm(2), respectively. Following ApPDT, cells were prepared for flow cytometry and transmission electron microscopy to unravel the mechanisms of cell death. Our results showed the lowest MB concentration (50 mu M) and the lowest optical power (100 mW) promoted the highest percentage of cell decrease. ApPDT caused alterations on cell membrane permeability as well depolarization of mitochondrial membrane potential. We also observed ultrastructural changes of the parasites such as cell shrinkage, intense vacuolization of the cytoplasm, enlargement of mitochondrion-kinetoplast complex, and small blebs on parasite flagella and cell membrane after MB-mediated ApPDT. Taken together, our findings ratify that ApPDT parameters play a pivotal role in cell susceptibility and suggest that apoptosis is involved in parasite death regardless MB-mediated ApPDT protocol.
dc.description.indexMEDLINE
dc.description.sponsorshipInstitute of Photonics from the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [INCT-573916/2008]
dc.description.sponsorshipCNPq
dc.identifier.citationPHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY, v.23, p.1-8, 2018
dc.identifier.doi10.1016/j.pdpdt.2018.05.005
dc.identifier.eissn1873-1597
dc.identifier.issn1572-1000
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/29571
dc.language.isoeng
dc.publisherELSEVIER SCIENCE BV
dc.relation.ispartofPhotodiagnosis and Photodynamic Therapy
dc.rightsrestrictedAccess
dc.rights.holderCopyright ELSEVIER SCIENCE BV
dc.subjectApoptosis
dc.subjectFlow cytometry
dc.subjectMembrane fluidity
dc.subjectMembrane potential
dc.subjectMicroscopy
dc.subjectPhotodynamic therapy
dc.subjectCutaneous leishmaniasis
dc.subject.othermucocutaneous leishmaniasis
dc.subject.otherinfantum promastigotes
dc.subject.othervitro
dc.subject.otherbraziliensis
dc.subject.otherefficiency
dc.subject.otherapoptosis
dc.subject.otherphotosensitizer
dc.subject.otherderivatives
dc.subject.wosOncology
dc.titleCell death mechanisms in Leishmania amazonensis triggered by methylene blue-mediated antiparasitic photodynamic therapy
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalAURELIANO, Debora P.:IPEN CNEN SP, Ctr Lasers & Applicat, Sao Paulo, SP, Brazil
hcfmusp.author.externalSOARES, Sandra Regina de Castro:Inst Infectol Emilio Ribas SES SP, Sao Paulo, SP, Brazil
hcfmusp.author.externalPEREIRA, Thiago Martini:Univ Fed Sao Paulo UNIFESP, Dept Ciencia & Tecnol, Sao Jose Dos Campos, SP, Brazil
hcfmusp.author.externalRIBEIRO, Martha Simoes:IPEN CNEN SP, Ctr Lasers & Applicat, Sao Paulo, SP, Brazil
hcfmusp.citation.scopus26
hcfmusp.contributor.author-fmusphcJOSE ANGELO LAULETTA LINDOSO
hcfmusp.contributor.author-fmusphcCLEUSA FUMICA HIRATA TAKAKURA
hcfmusp.description.beginpage1
hcfmusp.description.endpage8
hcfmusp.description.volume23
hcfmusp.origemWOS
hcfmusp.origem.pubmed29751117
hcfmusp.origem.scopus2-s2.0-85047319752
hcfmusp.origem.wosWOS:000446285400001
hcfmusp.publisher.cityAMSTERDAM
hcfmusp.publisher.countryNETHERLANDS
hcfmusp.relation.referenceAureliano D. P., LEISHMANIASIS TRENDS
hcfmusp.relation.referenceBacellar IOL, 2014, PHOTOCHEM PHOTOBIOL, V90, P801, DOI 10.1111/php.12264
hcfmusp.relation.referenceDavid CV, 2009, DERMATOL THER, V22, P491, DOI 10.1111/j.1529-8019.2009.01272.x
hcfmusp.relation.referenceDoroodgar M, 2016, KOREAN J PARASITOL, V54, P9, DOI 10.3347/kjp.2016.54.1.9
hcfmusp.relation.referenceDuszenko M, 2006, TRENDS PARASITOL, V22, P536, DOI 10.1016/j.pt.2006.08.010
hcfmusp.relation.referenceGalluzzi L, 2009, CELL DEATH DIFFER, V16, P1093, DOI 10.1038/cdd.2009.44
hcfmusp.relation.referenceGoto H, 2012, INFECT DIS CLIN N AM, V26, P293, DOI 10.1016/j.idc.2012.03.001
hcfmusp.relation.referenceGrivicich A., 2007, REV BRAS CANCEROL, V53, P335
hcfmusp.relation.referenceHamblin MR, 2016, CURR OPIN MICROBIOL, V33, P67, DOI 10.1016/j.mib.2016.06.008
hcfmusp.relation.referenceHorne TK, 2017, ANTICANCER RES, V37, P2785, DOI 10.21873/anticanres.11630
hcfmusp.relation.referenceJimenez-Ruiz A, 2010, PARASITE VECTOR, V3, DOI 10.1186/1756-3305-3-104
hcfmusp.relation.referenceKhademvatan S, 2013, EXP PARASITOL, V135, P208, DOI 10.1016/j.exppara.2013.07.004
hcfmusp.relation.referenceLopes Milene Valeria, 2012, Evid Based Complement Alternat Med, V2012, P298320, DOI 10.1155/2012/298320
hcfmusp.relation.referenceLu Y, 2008, J CELL BIOCHEM, V105, P1451, DOI 10.1002/jcb.21965
hcfmusp.relation.referenceWanderley JLM, 2009, PLOS ONE, V4, DOI 10.1371/journal.pone.0005733
hcfmusp.relation.referenceMontoya A, 2015, ANTIMICROB AGENTS CH, V59, P5804, DOI 10.1128/AAC.00545-15
hcfmusp.relation.referenceNunez SC, 2014, PHOTOCH PHOTOBIO SCI, V13, P595, DOI 10.1039/c3pp50325a
hcfmusp.relation.referencePinto JG, 2017, PHOTODIAGN PHOTODYN, V18, P325, DOI 10.1016/j.pdpdt.2017.04.009
hcfmusp.relation.referencePinto JG, 2016, LASER MED SCI, V31, P883, DOI 10.1007/s10103-016-1928-5
hcfmusp.relation.referencePrates RA, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0054387
hcfmusp.relation.referencePrates RA, 2009, LASER PHYS, V19, P1038, DOI 10.1134/S1054660X09050284
hcfmusp.relation.referenceProto WR, 2013, NAT REV MICROBIOL, V11, P58, DOI 10.1038/nrmicro2929
hcfmusp.relation.referenceRodrigues IA, 2014, BIOMED RES INT, DOI 10.1155/2014/985171
hcfmusp.relation.referenceSchmidt TF, 2015, LANGMUIR, V31, P4205, DOI 10.1021/acs.langmuir.5b00166
hcfmusp.relation.referenceSchuster FL, 2002, CLIN MICROBIOL REV, V15, P374, DOI 10.1128/CMR.15.3.374-389.2002
hcfmusp.relation.referenceSilva SCSPE, 2016, EXP PARASITOL, V163, P68, DOI 10.1016/j.exppara.2016.01.007
hcfmusp.relation.referenceSouza DM, 2017, PARASITE IMMUNOL, V39, DOI 10.1111/pim.12403
hcfmusp.relation.referenceTardivo JP, 2005, PHOTODIAGN PHOTODYN, V2, P175, DOI 10.1016/S1572-1000(05)00097-9
hcfmusp.relation.referenceTixeira R, 2017, APOPTOSIS, V22, P475, DOI 10.1007/s10495-017-1345-7
hcfmusp.relation.referenceTorres-Guerrero Edoardo, 2017, F1000Res, V6, P750, DOI 10.12688/f1000research.11120.1
hcfmusp.relation.referenceUsacheva MN, 2003, J PHOTOCH PHOTOBIO B, V71, P87, DOI 10.1016/j.jphotobiol.2003.06.002
hcfmusp.relation.referenceWorld Health Organization (WHO), 2017, GLOB OBS HLTH R D PR
hcfmusp.relation.referenceYamamoto ES, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0144946
hcfmusp.relation.referenceZauli-Nascimento RC, 2010, TROP MED INT HEALTH, V15, P68, DOI 10.1111/j.1365-3156.2009.02414.x
hcfmusp.scopus.lastupdate2024-05-10
relation.isAuthorOfPublication1c8fa285-4408-4b15-9ed4-a7209ef85fc5
relation.isAuthorOfPublication240d365d-be66-44e2-a122-f56bd4a788d7
relation.isAuthorOfPublication.latestForDiscovery1c8fa285-4408-4b15-9ed4-a7209ef85fc5
Arquivos
Pacote Original
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
art_AURELIANO_Cell_death_mechanisms_in_Leishmania_amazonensis_triggered_by_2018.PDF
Tamanho:
4.14 MB
Formato:
Adobe Portable Document Format
Descrição:
publishedVersion (English)
Baixar
Coleções
Artigos e Materiais de Revistas Científicas - FM/MPT
Artigos e Materiais de Revistas Científicas - IMT
Artigos e Materiais de Revistas Científicas - LIM/38