Analysis of an iodide radioimmunoassay for 11-deoxicortisol measurement

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art_MADEIRA_Analysis_of_an_iodide_radioimmunoassay_for_11_deoxicortisol_2014.PDF (250.32 KB)
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1
Tipo de produção
article
Data de publicação
2014
DOI
SciELO
Scopus
Web of Science
PubMed
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Editora
SBEM-SOC BRASIL ENDOCRINOLOGIA & METABOLOGIA
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Citação
ARQUIVOS BRASILEIROS DE ENDOCRINOLOGIA E METABOLOGIA, v.58, n.3, p.232-236, 2014
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Resumo
Objective: Our aim was to correlate 11-deoxycortisol levels obtained by two currently available techniques for 11-deoxycortisol measurement: radioimmunoassay, and high performance liquid chromatography followed by tandem mass spectrometry (MS/MS). The latter is the gold standard method for steroid hormone measurement. Materials and methods: We selected 88 samples and the results of these two methods were compared by Deming regression. Results: The analytical sensitivity of the RIA was 0.30 ng/mL, with inadequate linearity and inadequate precision profile (34% of the samples had a CV >= 20%). From the selected samples, 54 had measurable levels of 11-deoxycortisol in both methods and were used in the comparison. The comparison of RIA with LC-MS/MS showed an overestimation of the results by RIA. The correlation coefficient was 0.610; linear regression slope was 3.751; and the intercept was 0.145, indicating a poor correlation between the two methods. Conclusion: We concluded that 11-deoxycortisol measured by radioimmunoassay, despite a good analytical sensitivity, showed very low specificity, precluding its use as a reliable method for 11-deoxycortisol measurement.
Palavras-chave
11-deoxycortisol, radioimmunoassay, mass spectrometry, congenital adrenal hyperplasia
URI
https://observatorio.fm.usp.br/handle/OPI/7352
Referências
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MCM
Artigos e Materiais de Revistas Científicas - HC/ICESP
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/42