Engraftment of human adipose derived stem cells delivered in a hyaluronic acid preparation in mice

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorDIETRICH, Isa
dc.contributor.authorCOCHET, Olivia
dc.contributor.authorVILLAGEOIS, Phi
dc.contributor.authorRODRIGUES, Consuelo Junqueira
dc.date.accessioned2013-10-02T19:31:12Z
dc.date.available2013-10-02T19:31:12Z
dc.date.issued2012
dc.description.abstractPURPOSE: To evaluate the implant of human adipose derived stem cells (ADSC) delivered in hyaluronic acid gel (HA), injected in the subcutaneous of athymic mice. METHODS: Control implants -HA plus culture media was injected in the subcutaneous of the left sub scapular area of 12 athymic mice. ADSC implants: HA plus ADSC suspended in culture media was injected in the subcutaneous, at the contra lateral area, of the same animals. With eight weeks, animals were sacrificed and the recovered implants were processed for extraction of genomic DNA, and histological study by hematoxilin-eosin staining and immunufluorescence using anti human vimentin and anti von Willebrand factor antibodies. RESULTS: Controls: Not visualized at the injection site. An amorphous substance was observed in hematoxilin-eosin stained sections. Human vimentin and anti von Willebrand factor were not detected. No human DNA was detected. ADSC implants - A plug was visible at the site of injection. Fusiform cells were observed in sections stained by hematoxilin-eosin and both human vimentin and anti von Willebrand factor were detected by immunofluorescence. The presence of human DNA was confirmed. CONCLUSION: The delivery of human adipose derived stem cells in preparations of hyaluronic acid assured cells engraftment at the site of injection.
dc.description.abstractOBJETIVO: Avaliar o implante de células tronco do tecido adiposo humano (CTTAH) em gel de ácido hialurônico (AH), injetados no tecido subcutâneo de camundongos atímicos. MÉTODOS: Implantes controle - HA com meio de cultura foram injetados no tecido subcutâneo da região infraescapular esquerda de 12 camundongos atímicos. Implantes de CTTAH: HA com CTTAH suspensas em meio de cultura foi injetado no subcutâneo da região contra lateral, dos mesmos animais. Com oito semanas, os animais foram sacrificados e os implantes recuperados foram processados para extração de DNA genômico, estudo histológico por coloração por hematoxilina eosina e imnuoflurescência utilizando anticorpos anti vimentina humana e anti fator de von Willebrand. RESULTADOS: Controles - implantes não visualizados no local da injeção. Uma substância amorfa foi observada nos cortes corados por hematoxilina eosina. Vimentina humana e fator anti von Willebrand não foram identificados. DNA humano não foi detectado. Implantes de CTTAH - Uma massa era visível no local da injeção. Células fusiformes foram observadas nos corte corados com hematoxilina eosina. Tanto vimentina humana quanto fator de von Willebrand foram identificados pela imunofluorescência. A presença de DNA humano foi confirmada. CONCLUSÃO: O implante de células tronco do tecido adiposo humano em veículo de ácido hialurônico gel assegurou a manutenção das células no local do implante.
dc.description.indexMEDLINE
dc.description.sponsorshipCo-ordination of Improvement for Higher Academic Staff (CAPES)
dc.identifier.citationACTA CIRURGICA BRASILEIRA, v.27, n.4, p.283-289, 2012
dc.identifier.doi10.1590/S0102-86502012000400001
dc.identifier.issn0102-8650
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/2560
dc.language.isoeng
dc.publisherACTA CIRURGICA BRASILEIRA
dc.relation.ispartofActa Cirurgica Brasileira
dc.rightsopenAccess
dc.rights.holderCopyright ACTA CIRURGICA BRASILEIRA
dc.subjectAdult Stem Cells
dc.subjectTissue Engineering
dc.subjectCell Transplantation
dc.subjectAdipose Tissue
dc.subjectHyaluronic Acid
dc.subjectBiocompatible Materials
dc.subjectMice
dc.subjectCélulas-Tronco Adultas
dc.subjectEngenharia Tecidual
dc.subjectTransplante de Células
dc.subjectTecido Adiposo
dc.subjectÁcido Hialurônico
dc.subjectMateriais Biocompatíveis
dc.subjectCamundongos
dc.subject.otherendothelial growth-factor
dc.subject.otherangiogenesis in-vitro
dc.subject.otherextracellular-matrix
dc.subject.othertissue
dc.subject.othermarrow
dc.subject.otherrepair
dc.subject.otherdamage
dc.subject.othermodel
dc.subject.othervivo
dc.subject.wosSurgery
dc.titleEngraftment of human adipose derived stem cells delivered in a hyaluronic acid preparation in mice
dc.title.alternativeImplante de células tronco do tecido adiposo humano numa preparação de ácido hialurônico em camundongos
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.affiliation.countryFrança
hcfmusp.affiliation.countryisofr
hcfmusp.author.externalDIETRICH, Isa:Univ Nice Sophia Antipolis, Inst Dev Biol & Canc, CNRS, Unite Mixte Rech UMR 6543, Nice, France
hcfmusp.author.externalCOCHET, Olivia:Univ Nice Sophia Antipolis, Inst Dev Biol & Canc, CNRS, Unite Mixte Rech UMR 6543, Nice, France
hcfmusp.author.externalVILLAGEOIS, Phi:Univ Nice Sophia Antipolis, Inst Dev Biol & Canc, CNRS, Unite Mixte Rech UMR 6543, Nice, France
hcfmusp.citation.scopus5
hcfmusp.contributor.author-fmusphcCONSUELO JUNQUEIRA RODRIGUES
hcfmusp.description.beginpage283
hcfmusp.description.endpage289
hcfmusp.description.issue4
hcfmusp.description.volume27
hcfmusp.origemWOS
hcfmusp.origem.pubmed22534801
hcfmusp.origem.scieloSCIELO:S0102-86502012000400001
hcfmusp.origem.scopus2-s2.0-84860708410
hcfmusp.origem.wosWOS:000303198700001
hcfmusp.publisher.citySAO PAULO
hcfmusp.publisher.countryBRAZIL
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hcfmusp.remissive.sponsorshipCAPES
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