Identification of bioactive peptides from a Brazilian kefir sample, and their anti-Alzheimer potential in Drosophila melanogaster

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorMALTA, S. M.
dc.contributor.authorBATISTA, L. L.
dc.contributor.authorSILVA, H. C. G.
dc.contributor.authorFRANCO, R. R.
dc.contributor.authorSILVA, M. H.
dc.contributor.authorRODRIGUES, T. S.
dc.contributor.authorCORREIA, L. I. V.
dc.contributor.authorMARTINS, M. M.
dc.contributor.authorVENTURINI, G.
dc.contributor.authorESPINDOLA, F. S.
dc.contributor.authorSILVA, M. V. da
dc.contributor.authorUEIRA-VIEIRA, C.
dc.date.accessioned2023-10-04T15:11:34Z
dc.date.available2023-10-04T15:11:34Z
dc.date.issued2022
dc.description.abstractAlzheimer’s disease (AD) is the most common form of dementia in the elderly, affecting cognitive, intellectual, and motor functions. Different hypotheses explain AD’s mechanism, such as the amyloidogenic hypothesis. Moreover, this disease is multifactorial, and several studies have shown that gut dysbiosis and oxidative stress influence its pathogenesis. Knowing that kefir is a probiotic used in therapies to restore dysbiosis and that the bioactive peptides present in it have antioxidant properties, we explored its biotechnological potential as a source of molecules capable of modulating the amyloidogenic pathway and reducing oxidative stress, contributing to the treatment of AD. For that, we used Drosophila melanogaster model for AD (AD-like flies). Identification of bioactive peptides in the kefir sample was made by proteomic and peptidomic analyses, followed by in vitro evaluation of antioxidant and acetylcholinesterase inhibition potential. Flies were treated and their motor performance, brain morphology, and oxidative stress evaluated. Finally, we performed molecular docking between the peptides found and the main pathology-related proteins in the flies. The results showed that the fraction with the higher peptide concentration was positive for the parameters evaluated. In conclusion, these results revealed these kefir peptide-rich fractions have therapeutic potential for AD. © 2022, The Author(s).eng
dc.description.indexMEDLINE
dc.description.indexPubMed
dc.description.indexScopus
dc.description.sponsorshipBiotechnology Institute of the University of Uberlândia
dc.description.sponsorshipConselho Nacional Científico e Tecnológico
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior, CAPES
dc.description.sponsorshipUniversidade Federal de Uberlândia, UFU
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico, CNPq, (303667/2021-4, 312812/2021-3)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais, FAPEMIG, (APQ-02766-17, PPM-00503-18)
dc.identifier.citationSCIENTIFIC REPORTS, v.12, n.1, article ID 11065, p, 2022
dc.identifier.doi10.1038/s41598-022-15297-1
dc.identifier.issn2045-2322
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/55914
dc.language.isoeng
dc.publisherNATURE RESEARCHeng
dc.relation.ispartofScientific Reports
dc.rightsopenAccesseng
dc.rights.holderCopyright NATURE RESEARCHeng
dc.subject.otheracetylcholinesteraseeng
dc.subject.otheralzheimer diseaseeng
dc.subject.otheramyloid beta-peptideseng
dc.subject.otheranimalseng
dc.subject.otherantioxidantseng
dc.subject.otherbrazileng
dc.subject.otherdrosophila melanogastereng
dc.subject.otherdysbiosiseng
dc.subject.otherkefireng
dc.subject.othermolecular docking simulationeng
dc.subject.otherpeptideseng
dc.subject.otherproteomicseng
dc.subject.otheracetylcholinesteraseeng
dc.subject.otheramyloid beta proteineng
dc.subject.otherantioxidanteng
dc.subject.otherkefireng
dc.subject.otherpeptideeng
dc.subject.otheralzheimer diseaseeng
dc.subject.otheranimaleng
dc.subject.otherbrazileng
dc.subject.otherchemistryeng
dc.subject.otherdrosophila melanogastereng
dc.subject.otherdysbiosiseng
dc.subject.othermetabolismeng
dc.subject.othermolecular dockingeng
dc.subject.otherproteomicseng
dc.titleIdentification of bioactive peptides from a Brazilian kefir sample, and their anti-Alzheimer potential in Drosophila melanogastereng
dc.typearticleeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
dspace.entity.typePublication
hcfmusp.author.externalMALTA, S. M.:Institute of Biotechnology, Federal University of Uberlândia, MG, Uberlândia, Brazil, Laboratory of Genetics, Institute of Biotechnology, Federal University of Uberlândia, Acre Street, 2E building, room 230, MG, Uberlândia, 38405-319, Brazil
hcfmusp.author.externalBATISTA, L. L.:Institute of Biotechnology, Federal University of Uberlândia, MG, Uberlândia, Brazil
hcfmusp.author.externalSILVA, H. C. G.:Institute of Biotechnology, Federal University of Uberlândia, MG, Uberlândia, Brazil
hcfmusp.author.externalFRANCO, R. R.:Institute of Biotechnology, Federal University of Uberlândia, MG, Uberlândia, Brazil
hcfmusp.author.externalSILVA, M. H.:Institute of Biotechnology, Federal University of Uberlândia, MG, Uberlândia, Brazil
hcfmusp.author.externalRODRIGUES, T. S.:Institute of Biotechnology, Federal University of Uberlândia, MG, Uberlândia, Brazil
hcfmusp.author.externalCORREIA, L. I. V.:Institute of Biotechnology, Federal University of Uberlândia, MG, Uberlândia, Brazil
hcfmusp.author.externalMARTINS, M. M.:Institute of Biotechnology, Federal University of Uberlândia, MG, Uberlândia, Brazil
hcfmusp.author.externalESPINDOLA, F. S.:Institute of Biotechnology, Federal University of Uberlândia, MG, Uberlândia, Brazil
hcfmusp.author.externalSILVA, M. V. da:Pró-Reitoria de Pesquisa e Pós-Graduação, Universidade Federal de Uberlândia, MG, Uberlândia, Brazil
hcfmusp.author.externalUEIRA-VIEIRA, C.:Institute of Biotechnology, Federal University of Uberlândia, MG, Uberlândia, Brazil, Laboratory of Genetics, Institute of Biotechnology, Federal University of Uberlândia, Acre Street, 2E building, room 230, MG, Uberlândia, 38405-319, Brazil
hcfmusp.citation.scopus16
hcfmusp.contributor.author-fmusphcGABRIELA VENTURINI DA SILVA
hcfmusp.description.articlenumber11065
hcfmusp.description.issue1
hcfmusp.description.volume12
hcfmusp.origemSCOPUS
hcfmusp.origem.pubmed35773306
hcfmusp.origem.scopus2-s2.0-85133134836
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