Endothelial, platelet, and macrophage microparticle levels do not change acutely following transcatheter aortic valve replacement

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorMARCHINI, Julio F.
dc.contributor.authorMIYAKAWA, Ayumi Aurea
dc.contributor.authorTARASOUTCHI, Flavio
dc.contributor.authorKRIEGER, Jose Eduardo
dc.contributor.authorLEMOS, Pedro
dc.contributor.authorCROCE, Kevin
dc.date.accessioned2016-12-20T16:36:25Z
dc.date.available2016-12-20T16:36:25Z
dc.date.issued2016
dc.description.abstractBackground: Patients with severe aortic stenosis have increased levels of prothrombotic and proinflammatory microparticles (MP), and MPs actively regulate pathological processes that lead to atherothrombotic cardiovascular events. Shear stress is a validated stimulus of MP production, and abnormal shear stress in aortic stenosis increases MP release in ex-vivo studies. We hypothesized that in patients with severe aortic stenosis, percutaneous replacement of the aortic valve (TAVR) would reduce abnormal shear stress and would decrease levels of circulating MPs. Findings: The experimental protocol utilized flow cytometry (FC) and nanoparticle tracking analysis (NTA) to quantify circulating plasma MP levels in aortic stenosis patients at baseline and 5 days after TAVR. The baseline and 5 day MP counts measured by FC were 6.10.10(5) +/- 1.21.10(5) MP/mu L and 5.74.10(5) +/- 9.54.10(4) MP/mu L, respectively (p = 0.91). The baseline and 5 day MP counts measured by NTA were 9.29.10(13) +/- 1.66.10(13) MP/mu L and 3.95.10(14) +/- 3.11.10(14) MP/mu L, respectively (p = 0.91). When MPs were stratified by cell source, there was no difference in pre/post TAVR endothelial, platelet, or leukocyte MP levels. Conclusion: Levels of circulating MPs do not change acutely following TAVR therapy for aortic stenosis. Trial registered at clinicaltrials. gov NCT02193035 on July 11, 2014.
dc.description.indexMEDLINE
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (BR)
dc.identifier.citationJOURNAL OF NEGATIVE RESULTS IN BIOMEDICINE, v.15, article ID 7, 5p, 2016
dc.identifier.doi10.1186/s12952-016-0051-2
dc.identifier.issn1477-5751
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/17030
dc.language.isoeng
dc.publisherBIOMED CENTRAL LTD
dc.relation.ispartofJournal of Negative Results in Biomedicine
dc.rightsopenAccess
dc.rights.holderCopyright BIOMED CENTRAL LTD
dc.subjectSevere aortic stenosis
dc.subjectMicroparticles
dc.subjectNanoparticle-tracking analysis
dc.subjectFlow cytometry
dc.subject.othernanoparticle tracking analysis
dc.subject.othercirculating microparticles
dc.subject.otheractivation
dc.subject.othervesicles
dc.subject.otherexosomes
dc.subject.othercells
dc.subject.wosMedicine, Research & Experimental
dc.titleEndothelial, platelet, and macrophage microparticle levels do not change acutely following transcatheter aortic valve replacement
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.affiliation.countryEstados Unidos
hcfmusp.affiliation.countryisous
hcfmusp.author.externalCROCE, Kevin:Harvard Med Sch, Brigham & Womens Hosp, Dept Med, Div Cardiovasc, Boston, MA USA
hcfmusp.citation.scopus7
hcfmusp.contributor.author-fmusphcJULIO FLAVIO MEIRELLES MARCHINI
hcfmusp.contributor.author-fmusphcAYUMI AUREA MIYAKAWA YAMAGUCHI
hcfmusp.contributor.author-fmusphcFLAVIO TARASOUTCHI
hcfmusp.contributor.author-fmusphcJOSE EDUARDO KRIEGER
hcfmusp.contributor.author-fmusphcPEDRO ALVES LEMOS NETO
hcfmusp.description.articlenumber7
hcfmusp.description.volume15
hcfmusp.origemWOS
hcfmusp.origem.pubmed27063005
hcfmusp.origem.scopus2-s2.0-84964547525
hcfmusp.origem.wosWOS:000386477500001
hcfmusp.publisher.cityLONDON
hcfmusp.publisher.countryENGLAND
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