Validation of ELISA with recombinant antigens in serological diagnosis of canine Leishmania infantum infection

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art_FUJIMORI_Validation_of_ELISA_with_recombinant_antigens_in_serological_2021.PDF (2.88 MB)
Citações na Scopus
5
Tipo de produção
article
Data de publicação
2021
DOI
SciELO
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
FUNDACO OSWALDO CRUZ
Autores
ALMEIDA, Arleana do Bom Parto Ferreira de
BARROUIN-MELO, Stella Maria
CORTEZ, Luiz Ricardo Paes de Barros
DUTHIE, Malcolm Scott
HIRAMOTO, Roberto Mitsuyoshi
PINHO, Flaviane Alves de
REED, Steven Gregory
SOUSA, Valeria Regia Franco
SOUZA, Nazare Fonseca
Citação
MEMORIAS DO INSTITUTO OSWALDO CRUZ, v.116, article ID e200428, 13p, 2021
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
BACKGROUND Dogs are the main peridomiciliary reservoir of Leishmania infantum thus the correct diagnosis of infection is essential for the control of the transmission and treatment as well. However, the diagnosis is based on serological assays that are not fully effective. OBJECTIVE We aimed to establish an effective serological assay for the diagnosis of L. infantum infected dogs using Leishmania-derived recombinant antigens. METHODS Leishmania derived rK39-, rK28-, rKR95-based enzyme-linked immunosorbent assay (ELISA) was standardized using symptomatic and asymptomatic L. infantum-infected dogs. Then 2,530 samples from inquiry in endemic areas for VL were evaluated and the results compared with recommended assays by the Brazilian Ministry of Health (MH algorithm). Further samples from a cohort of 30 dogs were searched. FINDINGS For rK39-, rK28- and rKR95-ELISA the sensitivity was around 97% and specificity 100%. The positivity of these three ELISA in the inquiry samples was 27-28%, around 10% higher than the assays currently in use. When cohort samples were searched, we observed likely false-negative results (> 65%) with supposedly negative samples that turned positive six months later with the assays in use (MH algorithm). MAIN CONCLUSIONS For the diagnosis of L. infantum-infected dogs, rK39-based ELISA showed better diagnostic performance than other assays in use in Brazil and worldwide.
Palavras-chave
canine visceral leishmaniasis, diagnosis, recombinant antigens, ELISA, epidemiological inquiry
URI
https://observatorio.fm.usp.br/handle/OPI/40807
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MPR
Artigos e Materiais de Revistas Científicas - IMT
Artigos e Materiais de Revistas Científicas - LIM/38
Artigos e Materiais de Revistas Científicas - ODS/07