Beneficial effects of IL-4 and IL-6 on rat neonatal target cardiac cells

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorZOGBI, Camila
dc.contributor.authorOLIVEIRA, Nathalia C.
dc.contributor.authorLEVY, Debora
dc.contributor.authorBYDLOWSKI, Sergio P.
dc.contributor.authorBASSANEZE, Vinicius
dc.contributor.authorNERI, Elida A.
dc.contributor.authorKRIEGER, Jose E.
dc.date.accessioned2020-10-15T14:30:43Z
dc.date.available2020-10-15T14:30:43Z
dc.date.issued2020
dc.description.abstractThe nature of the early post-natal immune response in rodents appears to influence cardiac regeneration even though the underlying molecules remain poorly understood. Consistent with this idea, we show now significant changes in the expression of immune and cell movement gene pathways in heart samples from 1- and 7-day-old rats with ventricle resection. We then tested whether conditioned media from adult M2 anti-inflammatory macrophages target neonatal cardiac cells to a pro-regenerative like phenotype compared to the M1 pro-inflammatory macrophages. We found that M2 compared to M1 macrophage-conditioned media upregulates neonatal cardiomyocyte proliferation, suppresses myofibroblast-induced differentiation and stimulates endothelial cell tube formation. Using a cytokine array, we selected four candidate cytokine molecules uniquely expressed in M2 macrophage-conditioned media and showed that two of them (IL-4 and IL-6) induce endothelial cell proliferation whilst IL-4 promotes proliferation in neonatal cardiomyocytes and prevents myofibroblast-induced collagen type I secretion. Altogether, we provided evidence that adult M2 macrophage-conditioned media displays a paracrine beneficial pro-regenerative response in target cardiac cells and that IL-4 and IL-6 recapitulate, at least in part, these pleiotropic effects. Further characterization of macrophage immune phenotypes and their secreted molecules may give rise to novel therapeutic approaches for post-natal cardiac repair.eng
dc.description.indexMEDLINEeng
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2013/17368-0, 15/50216-5, 2017/07024-3, 2017/17296-0]
dc.description.sponsorshipConselho Nacional de Pesquisa (CNPq)National Council for Scientific and Technological Development (CNPq) [309179/2013-0]
dc.identifier.citationSCIENTIFIC REPORTS, v.10, n.1, article ID 12350, 12p, 2020
dc.identifier.doi10.1038/s41598-020-69413-0
dc.identifier.issn2045-2322
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/37635
dc.language.isoeng
dc.publisherNATURE PUBLISHING GROUPeng
dc.relation.ispartofScientific Reports
dc.rightsopenAccesseng
dc.rights.holderCopyright NATURE PUBLISHING GROUPeng
dc.subject.otherread alignmenteng
dc.subject.othermacrophageseng
dc.subject.otherinflammationeng
dc.subject.othercontributeeng
dc.subject.otherrepaireng
dc.subject.otherhearteng
dc.subject.wosMultidisciplinary Scienceseng
dc.titleBeneficial effects of IL-4 and IL-6 on rat neonatal target cardiac cellseng
dc.typearticleeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
dspace.entity.typePublication
hcfmusp.author.externalBASSANEZE, Vinicius:Univ Sao Paulo, Heart Inst InCor, Sch Med, Lab Genet & Mol Cardiol LIM 13, Av Dr Eneas C Aguiar 44, BR-05403000 Sao Paulo, SP, Brazil
hcfmusp.citation.scopus14
hcfmusp.contributor.author-fmusphcCAMILA ZOGBI NOGUEIRA PEREZ
hcfmusp.contributor.author-fmusphcNATHALIA CORREA DE ALMEIDA OLIVEIRA
hcfmusp.contributor.author-fmusphcDEBORA LEVY
hcfmusp.contributor.author-fmusphcSERGIO PAULO BYDLOWSKI
hcfmusp.contributor.author-fmusphcELIDA ADALGISA NERI
hcfmusp.contributor.author-fmusphcJOSE EDUARDO KRIEGER
hcfmusp.description.articlenumber12350
hcfmusp.description.issue1
hcfmusp.description.volume10
hcfmusp.origemWOS
hcfmusp.origem.pubmed32704142
hcfmusp.origem.scopus2-s2.0-85088370759
hcfmusp.origem.wosWOS:000556690900057
hcfmusp.publisher.cityLONDONeng
hcfmusp.publisher.countryENGLANDeng
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