Low level laser therapy increases angiogenesis in a model of ischemic skin flap in rats mediated by VEGF, HIF-1 alpha and MMP-2

Imagem de Miniatura
Arquivos
art_MORETTI_Low_level_laser_therapy_increases_angiogenesis_in_a_2013.PDF (1004.92 KB)
Citações na Scopus
138
Tipo de produção
article
Data de publicação
2013
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
ELSEVIER SCIENCE SA
Autores
CURY, Vivian
ASSIS, Livia
BOSSINI, Paulo
CRUSCA, Jaqueline de Souza
BENATTI NETO, Carlos
FANGEL, Renan
HAMBLIN, Michael R.
PARIZOTTO, Nivaldo Antonio
Citação
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v.125, p.164-170, 2013
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
It is known that low level laser therapy is able to improve skin flap viability by increasing angiogenesis. However, the mechanism for new blood vessel formation is not completely understood. Here, we investigated the effects of 660 nm and 780 nm lasers at fluences of 30 and 40 J/cm(2) on three important mediators activated during angiogenesis. Sixty male Wistar rats were used and randomly divided into five groups with twelve animals each. Groups were distributed as follows: skin flap surgery non-irradiated group as a control; skin flap surgery irradiated with 660 nm laser at a fluence of 30 or 40 J/cm(2) and skin flap surgery irradiated with 780 nm laser at a fluence of 30 or 40 J/cm(2). The random skin flap was performed measuring 10 x 4 cm, with a plastic sheet interposed between the flap and the donor site. Laser irradiation was performed on 24 points covering the flap and surrounding skin immediately after the surgery and for 7 consecutive days thereafter. Tissues were collected, and the number of vessels, angiogenesis markers (vascular endothelial growth factor, VEGF and hypoxia inducible factor, HIF-1 alpha) and a tissue remodeling marker (matrix metalloproteinase, MMP-2) were analyzed. LLLT increased an angiogenesis, HIF-1 alpha and VEGF expression and decrease MMP-2 activity. These phenomena were dependent on the fluences, and wavelengths used. In this study we showed that LLLT may improve the healing of skin flaps by enhancing the amount of new vessels formed in the tissue. Both 660 nm and 780 nm lasers were able to modulate VEGF secretion, MMP-2 activity and HIF-1 alpha expression in a dose dependent manner.
Palavras-chave
LLLT, Angiogenesis, HIF1-alpha, VEGF, MMP-2
URI
https://observatorio.fm.usp.br/handle/OPI/3904
Referências
  1. Ankri R, 2010, LASER SURG MED, V42, P760, DOI 10.1002/lsm.20955
  2. Bossini PS, 2009, LASER MED SCI, V24, P209, DOI 10.1007/s10103-008-0551-5
  3. Botusan IR, 2008, P NATL ACAD SCI USA, V105, P19426, DOI 10.1073/pnas.0805230105
  4. Carroll VA, 2006, CANCER RES, V66, P6264, DOI 10.1158/0008-5472.CAN-05-2519
  5. Conway EM, 2001, CARDIOVASC RES, V49, P507, DOI 10.1016/S0008-6363(00)00281-9
  6. Corazza AV, 2007, PHOTOMED LASER SURG, V25, P102, DOI 10.1089/pho.2006.2011
  7. Correa F, 2007, PHOTOMED LASER SURG, V25, P245, DOI 10.1089/pho.2007.2079
  8. Cury V, 2009, PHOTOMED LASER SURG, V27, P721, DOI 10.1089/pho.2008.2383
  9. Demidova-Rice TN, 2007, LASER SURG MED, V39, P706, DOI 10.1002/lsm.20549
  10. ENWEMEKA CS, 1990, ULTRASOUND MED BIOL, V16, P801, DOI 10.1016/0301-5629(90)90044-D
  11. Fang JM, 2001, CANCER RES, V61, P5731
  12. Gu ZZ, 2002, SCIENCE, V297, P1186, DOI 10.1126/science.1073634
  13. Hamik A, 2006, ARTERIOSCL THROM VAS, V26, P1936, DOI 10.1161/01.ATV.0000232542.42968.e3
  14. KERRIGAN CL, 1983, PLAST RECONSTR SURG, V72, P766, DOI 10.1097/00006534-198312000-00003
  15. Kipshidze N, 2001, LASER SURG MED, V28, P355, DOI 10.1002/lsm.1062
  16. Lizarbe TR, 2008, FASEB J, V22, P3207, DOI 10.1096/fj.07-103804
  17. Mesquita-Ferrari R.A., 2010, LASERS MED SCI
  18. Nguyen M, 2001, INT J BIOCHEM CELL B, V33, P960, DOI 10.1016/S1357-2725(01)00007-3
  19. Paduch R, 2005, CELL BIOL INT, V29, P497, DOI 10.1016/j.cellbi.2005.01.007
  20. Pinfildi CE, 2005, LASER SURG MED, V37, P74, DOI 10.1002/lsm.20190
  21. Pinheiro Antonio Luiz Barbosa, 2004, Braz Dent J, V15 Spec No, pSI21
  22. Prado Rodrigo Paschoal, 2006, Acta Cir Bras, V21, P258, DOI 10.1590/S0102-86502006000400013
  23. Rivilis I, 2002, AM J PHYSIOL-HEART C, V283, pH1430, DOI 10.1152/ajpheart.00082.2002
  24. Skinner SM, 1996, AUST DENT J, V41, P188
  25. Srinivas V, 1998, J BIOL CHEM, V273, P18019, DOI 10.1074/jbc.273.29.18019
  26. Stetler-Stevenson W.G., 1999, J CLIN INVEST, V103, P1237
  27. Tang N, 2004, CANCER CELL, V6, P485, DOI 10.1016/j.ccr.2004.09.026
  28. Tuby H, 2006, LASER SURG MED, V38, P682, DOI 10.1002/lsm.20377
  29. Tuby H, 2009, PHOTOMED LASER SURG, V27, P227, DOI 10.1089/pho.2008.2272

Coleções
Artigos e Materiais de Revistas Científicas - FM/MCM
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/51