The recombinant LIC10508 is a plasma fibronectin, plasminogen, fibrinogen and C4BP-binding protein of Leptospira interrogans

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorSIQUEIRA, Gabriela H.
dc.contributor.authorTEIXEIRA, Aline F.
dc.contributor.authorFERNANDES, Luis G.
dc.contributor.authorSOUZA, Gisele O. de
dc.contributor.authorKIRCHGATTER, Karin
dc.contributor.authorROMERO, Eliete C.
dc.contributor.authorVASCONCELLOS, Silvio A.
dc.contributor.authorVIEIRA, Monica L.
dc.contributor.authorNASCIMENTO, Ana Lucia T. O.
dc.date.accessioned2016-07-18T13:11:36Z
dc.date.available2016-07-18T13:11:36Z
dc.date.issued2016
dc.description.abstractLeptospirosis is a zoonosis caused by pathogenic Leptospira spp. In this study, we report that the recombinant proteins LIC10507, LIC10508 and LIC10509 are recognized by confirmed leptospirosis serum samples at both phases of the disease. The recombinant rLIC10508 and rLIC10507 are plasminogen (PLG)-binding proteins, capable of generating plasmin in the presence of a PLG activator. The proteins bind to PLG in a dose-dependent and saturable manner, fulfilling host-ligand interaction. Furthermore, rLIC10508 interacts with fibrinogen (Fg), plasma fibronectin and C4b binding protein (C4BP). The binding of rLIC10508 to Fg decreases the fibrin clotting in a thrombin-catalyzed reaction. The incubation with 4 mu M of protein promoted 40% inhibition upon clotting formation. C4BP bound to rLIC10508 retained its cofactor activity for factor I promoting the cleavage of C4b protein, which may reduce the membrane attack complex formation. Although these proteins have high amino acid sequence similarity, rLIC10508 is the most talented of the three, a behavior that might be explained by its unique putative 3D structure, whereas structures of rLIC10507 and rLIC10509 are very similar. Plasmin generation (rLIC10507 and rLIC10508), together with decreasing fibrin clot formation (rLIC10508) and impairment of the complement system (rLIC10508) may help the bacteria to overcome host defense, facilitating the infection process.
dc.description.indexMEDLINE
dc.description.sponsorshipFAPESP [12/23913-9]
dc.description.sponsorshipCNPq [302758/2013-5, 441449/2014-0]
dc.description.sponsorshipFundacao Butantan, Brazil
dc.description.sponsorshipFAPESP (Brazil) [2014/03792-8, 2012/50523-7, 2014/18337-4, 2012/01797-7, 2012/24164-0]
dc.identifier.citationPATHOGENS AND DISEASE, v.74, n.2, article ID UNSP ftv118, 11p, 2016
dc.identifier.doi10.1093/femspd/ftv118
dc.identifier.issn2049-632X
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/14598
dc.language.isoeng
dc.publisherOXFORD UNIV PRESS
dc.relation.ispartofPathogens and Disease
dc.rightsrestrictedAccess
dc.rights.holderCopyright OXFORD UNIV PRESS
dc.subjectLeptospira
dc.subjectleptospirosis
dc.subjectplasmin
dc.subjectfibrinogen
dc.subjectC4BP
dc.subjecthost interactions
dc.subject.othercomplement regulator c4bp
dc.subject.otherin-vitro
dc.subject.otherendothelial-cells
dc.subject.otherbinding protein
dc.subject.otherimmune evasion
dc.subject.otherclot formation
dc.subject.otherpathogenesis
dc.subject.otherinfection
dc.subject.othersurface
dc.subject.otheractivation
dc.subject.wosImmunology
dc.subject.wosInfectious Diseases
dc.subject.wosMicrobiology
dc.titleThe recombinant LIC10508 is a plasma fibronectin, plasminogen, fibrinogen and C4BP-binding protein of Leptospira interrogans
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalSIQUEIRA, Gabriela H.:Inst Butantan, Ctr Biotecnol, Ave Vital Brazil 1500, BR-05503900 Sao Paulo, SP, Brazil
hcfmusp.author.externalTEIXEIRA, Aline F.:Inst Butantan, Ctr Biotecnol, Ave Vital Brazil 1500, BR-05503900 Sao Paulo, SP, Brazil; Univ Sao Paulo, ICB, Programa Posgrad Interunidades Biotecnol, Ave Prof Lineu Prestes 1730, BR-05503900 Sao Paulo, SP, Brazil
hcfmusp.author.externalFERNANDES, Luis G.:Inst Butantan, Ctr Biotecnol, Ave Vital Brazil 1500, BR-05503900 Sao Paulo, SP, Brazil; Univ Sao Paulo, ICB, Programa Posgrad Interunidades Biotecnol, Ave Prof Lineu Prestes 1730, BR-05503900 Sao Paulo, SP, Brazil
hcfmusp.author.externalSOUZA, Gisele O. de:Univ Sao Paulo, Fac Med Vet & Zootecnia, Lab Zoonoses Bacterianas VPS, Ave Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo, SP, Brazil
hcfmusp.author.externalROMERO, Eliete C.:Adolfo Lutz Inst, Ctr Bacteriol, Ave Dr Arnaldo 355, BR-01246902 Sao Paulo, SP, Brazil
hcfmusp.author.externalVASCONCELLOS, Silvio A.:Univ Sao Paulo, Fac Med Vet & Zootecnia, Lab Zoonoses Bacterianas VPS, Ave Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo, SP, Brazil
hcfmusp.author.externalVIEIRA, Monica L.:Inst Butantan, Ctr Biotecnol, Ave Vital Brazil 1500, BR-05503900 Sao Paulo, SP, Brazil
hcfmusp.author.externalNASCIMENTO, Ana Lucia T. O.:Inst Butantan, Ctr Biotecnol, Ave Vital Brazil 1500, BR-05503900 Sao Paulo, SP, Brazil; Univ Sao Paulo, ICB, Programa Posgrad Interunidades Biotecnol, Ave Prof Lineu Prestes 1730, BR-05503900 Sao Paulo, SP, Brazil
hcfmusp.citation.scopus12
hcfmusp.contributor.author-fmusphcKARIN KIRCHGATTER
hcfmusp.description.articlenumberUNSP ftv118
hcfmusp.description.issue2
hcfmusp.description.volume74
hcfmusp.origemWOS
hcfmusp.origem.pubmed26657108
hcfmusp.origem.scopus2-s2.0-84992107193
hcfmusp.origem.wosWOS:000371929200007
hcfmusp.publisher.cityOXFORD
hcfmusp.publisher.countryENGLAND
hcfmusp.relation.referenceAdler B, 2011, VET MICROBIOL, V153, P73, DOI 10.1016/j.vetmic.2011.02.055
hcfmusp.relation.referenceLiu YY, 2007, MED MICROBIOL IMMUN, V196, P233, DOI 10.1007/s00430-007-0047-0
hcfmusp.relation.referenceVieira ML, 2011, MICROB PATHOGENESIS, V51, P360, DOI 10.1016/j.micpath.2011.06.008
hcfmusp.relation.referenceMendes RS, 2011, INFECT IMMUN, V79, P4657, DOI 10.1128/IAI.05583-11
hcfmusp.relation.referenceNicodemo AC, 1997, AM J TROP MED HYG, V56, P181
hcfmusp.relation.referencePlank R, 2000, MICROBES INFECT, V2, P1265, DOI 10.1016/S1286-4579(00)01280-6
hcfmusp.relation.referenceTHOMAS DD, 1990, INFECT IMMUN, V58, P581
hcfmusp.relation.referenceFernandes LGV, 2014, MICROBIOL-SGM, V160, P149, DOI 10.1099/mic.0.072074-0
hcfmusp.relation.referenceBharti AR, 2003, LANCET INFECT DIS, V3, P757, DOI 10.1016/S1473-3099(03)00830-2
hcfmusp.relation.referenceVerma A, 2006, INFECT IMMUN, V74, P2659, DOI 10.1128/IAI.74.5.2659-2666.2006
hcfmusp.relation.referenceJuncker AS, 2003, PROTEIN SCI, V12, P1652, DOI 10.1110/ps.0303703
hcfmusp.relation.referenceSiqueira GH, 2013, AM J TROP MED HYG, V89, P1103, DOI 10.4269/ajtmh.13-0205
hcfmusp.relation.referenceBarbosa AS, 2010, INFECT IMMUN, V78, P3207, DOI 10.1128/IAI.00279-10
hcfmusp.relation.referenceVieira ML, 2012, J BIOMED BIOTECHNOL, DOI 10.1155/2012/758513
hcfmusp.relation.referenceCINCO M, 1983, ZBL BAKT-INT J MED M, V254, P261
hcfmusp.relation.referenceLetunic I, 2015, NUCLEIC ACIDS RES, V43, pD257, DOI 10.1093/nar/gku949
hcfmusp.relation.referenceZhang L, 2012, MOL MICROBIOL, V83, P1006, DOI 10.1111/j.1365-2958.2012.07985.x
hcfmusp.relation.referenceHartskeerl RA, 2011, CLIN MICROBIOL INFEC, V17, P494, DOI 10.1111/j.1469-0691.2011.03474.x
hcfmusp.relation.referenceMeri T, 2005, MICROB PATHOGENESIS, V39, P139, DOI 10.1016/j.micpath.2005.07.003
hcfmusp.relation.referenceFernandes LGV, 2012, INFECT IMMUN, V80, P3679, DOI 10.1128/IAI.00474-12
hcfmusp.relation.referenceKoenigs A, 2013, J BIOL CHEM, V288, P25229, DOI 10.1074/jbc.M112.413872
hcfmusp.relation.referenceVieira ML, 2013, INFECT IMMUN, V81, P1764, DOI 10.1128/IAI.00020-13
hcfmusp.relation.referenceSegura ER, 2005, CLIN INFECT DIS, V40, P343, DOI 10.1086/427110
hcfmusp.relation.referenceBarthel D, 2012, J IMMUNOL, V188, P379, DOI 10.4049/jimmunol.1101927
hcfmusp.relation.referenceLahteenmaki K, 2001, FEMS MICROBIOL REV, V25, P531, DOI 10.1111/j.1574-6976.2001.tb00590.x
hcfmusp.relation.referenceBarbosa AS, 2009, INFECT IMMUN, V77, P1137, DOI 10.1128/IAI.01310-08
hcfmusp.relation.referenceGomez RM, 2008, MICROB PATHOGENESIS, V45, P315, DOI 10.1016/j.micpath.2008.08.004
hcfmusp.relation.referenceFinn RD, 2006, NUCLEIC ACIDS RES, V34, pD247, DOI 10.1093/nar/gkj149
hcfmusp.relation.referenceRen SX, 2003, NATURE, V422, P888, DOI 10.1038/nature01597
hcfmusp.relation.referenceRamos CRR, 2004, BRAZ J MED BIOL RES, V37, P1103, DOI 10.1590/S0100-879X2004000800001
hcfmusp.relation.referenceVieira ML, 2009, INFECT IMMUN, V77, P4092, DOI 10.1128/IAI.00353-09
hcfmusp.relation.referenceKo AI, 2009, NAT REV MICROBIOL, V7, P736, DOI 10.1038/nrmicro2208
hcfmusp.relation.referenceNascimento ALTO, 2004, J BACTERIOL, V186, P2164, DOI 10.1128/JB.186.7.2164-2172.2004
hcfmusp.relation.referenceEvangelista KV, 2010, FUTURE MICROBIOL, V5, P1413, DOI [10.2217/fmb.10.102, 10.2217/FMB.10.102]
hcfmusp.relation.referenceLevett PN, 2001, CLIN MICROBIOL REV, V14, P296, DOI 10.1128/CMR.14.2.296-326.2001
hcfmusp.relation.referenceBulach DM, 2006, P NATL ACAD SCI USA, V103, P14560, DOI 10.1073/pnas.0603979103
hcfmusp.relation.referenceLin YP, 2009, J BIOL CHEM, V284, P19380, DOI 10.1074/jbc.M109.004531
hcfmusp.relation.referenceSouza NM, 2012, MICROB PATHOGENESIS, V53, P125, DOI 10.1016/j.micpath.2012.06.001
hcfmusp.relation.referenceGamberini M, 2005, FEMS MICROBIOL LETT, V244, P305, DOI 10.1016/j.femsle.2005.02.004
hcfmusp.relation.referenceCinco M, 2010, NEW MICROBIOL, V33, P283
hcfmusp.relation.referenceLin YP, 2011, MOL MICROBIOL, V79, P1063, DOI 10.1111/j.1365-2958.2010.07510.x
hcfmusp.relation.referenceDomingos RF, 2015, MICROBIOL-SGM, V161, P851, DOI 10.1099/mic.0.000041
hcfmusp.relation.referencePinne M, 2012, J BACTERIOL, V194, P6074, DOI 10.1128/JB.01119-12
hcfmusp.relation.referencede Carvalho J E, 1992, Rev Soc Bras Med Trop, V25, P21
hcfmusp.relation.referenceDomingos RF, 2012, BMC MICROBIOL, V12, DOI 10.1186/1471-2180-12-50
hcfmusp.relation.referenceEvangelista K, 2014, PLOS NEGLECT TROP D, V8, DOI 10.1371/journal.pntd.0002672
hcfmusp.relation.referenceEvangelista KV, 2014, PLOS NEGLECT TROP D, V8, DOI 10.1371/journal.pntd.0003215
hcfmusp.relation.referenceFaine S, 1999, LEPTOSPIRA LEPTOSPIR, P272
hcfmusp.relation.referenceFigueira CP, 2011, BMC MICROBIOL, V11, DOI 10.1186/1471-2180-11-129
hcfmusp.relation.referenceHaake DA, 2002, CLIN INFECT DIS, V34, pE40, DOI 10.1086/339942
hcfmusp.relation.referenceKoch TK, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0047638
hcfmusp.relation.referenceNascimento AL, BRAZ J MED BIOL RES, V37, P459
hcfmusp.relation.referenceNogueira SV, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0078150
hcfmusp.relation.referenceOliveira R, 2013, PLOS NEGLECT TROP D, V7, DOI 10.1371/journal.pntd.0002396
hcfmusp.relation.referencePicardeau M, 2008, PLOS ONE, V3, DOI 10.1371/journal.pone.0001607
hcfmusp.relation.referenceSievers F, 2011, MOL SYST BIOL, V7, DOI 10.1038/msb.2011.75
hcfmusp.relation.referenceTeixeira AF, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0122762
hcfmusp.relation.referenceVieira ML, 2010, PLOS ONE, V5, DOI 10.1371/journal.pone.0011259
hcfmusp.relation.referenceVieira ML, 2015, CRIT REV MICROBIOL, DOI [10.3109/1040841X.2014.972336, DOI 10.3109/1040841X.2014.972336]
hcfmusp.relation.referenceWolff DG, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0081818
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