Endoplasmic Reticulum Stress and Autophagy Markers in Soleus Muscle Disuse-Induced Atrophy of Rats Treated with Fish Oil

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Arquivos
art_MARZUCA-NASSR_Endoplasmic_Reticulum_Stress_and_Autophagy_Markers_in_Soleus_2021.PDF (2.63 MB)
Citações na Scopus
7
Tipo de produção
article
Data de publicação
2021
DOI
Scopus
Web of Science
Título da Revista
ISSN da Revista
Título do Volume
Editora
MDPI
Autores
MARZUCA-NASSR, Gabriel Nasri
KUWABARA, Wilson Mitsuo Tatagiba
VITZEL, Kaio Fernando
TORRES, Rosangela Pavan
MANCINI-FILHO, Jorge
ALBA-LOUREIRO, Tatiana Carolina
CURI, Rui
Citação
NUTRIENTS, v.13, n.7, article ID 2298, 15p, 2021
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Endoplasmic reticulum stress (ERS) and autophagy pathways are implicated in disuse muscle atrophy. The effects of high eicosapentaenoic (EPA) or high docosahexaenoic (DHA) fish oils on soleus muscle ERS and autophagy markers were investigated in a rat hindlimb suspension (HS) atrophy model. Adult Wistar male rats received daily by gavage supplementation (0.3 mL per 100 g b.w.) of mineral oil or high EPA or high DHA fish oils (FOs) for two weeks. Afterward, the rats were subjected to HS and the respective treatments concomitantly for an additional two-week period. After four weeks, we evaluated ERS and autophagy markers in the soleus muscle. Results were analyzed using two-way analysis of variance (ANOVA) and Bonferroni post hoc test. Gastrocnemius muscle omega-6/omega-3 fatty acids (FAs) ratio was decreased by both FOs indicating the tissue incorporation of omega-3 fatty acids. HS altered (p < 0.05) the protein content (decreasing total p38 and BiP and increasing p-JNK2/total JNK2 ratio, and caspase 3) and gene expressions (decreasing BiP and increasing IRE1 and PERK) of ERS and autophagy (decreasing Beclin and increasing LC3 and ATG14) markers in soleus. Both FOs attenuated (p < 0.05) the increase in PERK and ATG14 expressions induced by HS. Thus, both FOs could potentially attenuate ERS and autophagy in skeletal muscles undergoing atrophy.
Palavras-chave
omega-3 fatty acids, eicosapentanoic acid, docosahexaenoic acid, hindlimb suspension, skeletal muscle atrophy, unfolded protein response
URI
https://observatorio.fm.usp.br/handle/OPI/41271
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MCM
Artigos e Materiais de Revistas Científicas - LIM/29