An outcome analysis and long-term viability of cryopreserved cultured epidermal allografts. Assessment of the conservation of transplantable human skin allografts

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorSCHIOZER, Wandir Antonio
dc.contributor.authorGEMPERLI, Rolf
dc.contributor.authorMUEHLBAUER, Wolfgang
dc.contributor.authorMUNHOZ, Alexandre Mendonca
dc.contributor.authorFERREIRA, Marcus Castro
dc.date.accessioned2014-04-25T21:49:51Z
dc.date.available2014-04-25T21:49:51Z
dc.date.issued2013
dc.description.abstractPURPOSE: To assess the viability of cultured epithelium and preserved by freezing for periods varying from one month to one year. METHODS: Samples of cultured epithelium were incubated in cryoprotectant medium (Group A), packed in aluminum envelopes and packed in polystyrene boxes. The boxes were subjected to a temperature of-70 degrees C. After freezing for a period of time ranging from one to 12 months, cultured epithelial samples were assessed for their viability by vital staining (Trypan blue) and metabolic analysis based on glucose consumption and lactate production. Samples of not frozen cultured epithelium (Group B) were also tested for viability and the results obtained were used as comparison parameter for the variation of viability. RESULTS: Statistical analysis between the group A and B indicate that the mean age of the donors (p=0.51) and the culture time (p=1.18) showed no statistical difference. In 30 days we obtained 37% of the original viability of cultured epithelium, 25% at six months and one year, less than 15%. This trend was confirmed statistically with a reduction of approximately 1.8% of the original viability epithelium cultured every 30 days of storage. In the analysis by lactate production, similar results were observed. In the analysis by the glucose consumption results were not significant. The viability indices show statistically significant difference between the group A and B (p<0.0001). CONCLUSIONS: Although cryopreserved cultured epithelium showed significant reduction of viability, all samples remained viable. It was also found that the viability of cryopreserved cultured epithelial decreased as a function of storage time.
dc.description.indexMEDLINE
dc.description.sponsorshipCoordination of Improvement for Higher Academic Staff (CAPES)
dc.identifier.citationACTA CIRURGICA BRASILEIRA, v.28, n.12, p.824-832, 2013
dc.identifier.doi10.1590/S0102-86502013001200004
dc.identifier.eissn1678-2674
dc.identifier.issn0102-8650
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/5052
dc.language.isoeng
dc.publisherACTA CIRURGICA BRASILEIRA
dc.relation.ispartofActa Cirurgica Brasileira
dc.rightsopenAccess
dc.rights.holderCopyright ACTA CIRURGICA BRASILEIRA
dc.subjectTissue Culture Techniques
dc.subjectTissue Banks
dc.subjectCryopreservation
dc.subjectBurns
dc.subject.othersplit-thickness skin
dc.subject.otherburn wounds
dc.subject.otherfresh
dc.subject.otherkeratinocytes
dc.subject.otherepithelium
dc.subject.othercells
dc.subject.other-70-degrees-c
dc.subject.othermetabolism
dc.subject.otherstorage
dc.subject.otherulcers
dc.subject.wosSurgery
dc.titleAn outcome analysis and long-term viability of cryopreserved cultured epidermal allografts. Assessment of the conservation of transplantable human skin allografts
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.affiliation.countryAlemanha
hcfmusp.affiliation.countryisode
hcfmusp.author.externalMUEHLBAUER, Wolfgang:Burn Ctr Klinikum Bogenhausen, Munich, Germany
hcfmusp.author.externalMUNHOZ, Alexandre Mendonca:Sirio Libanes Hosp, Postgrad Program Surg, Sao Paulo, Brazil
hcfmusp.citation.scopus13
hcfmusp.contributor.author-fmusphcWANDIR ANTONIO SCHIOZER
hcfmusp.contributor.author-fmusphcROLF GEMPERLI
hcfmusp.contributor.author-fmusphcMARCUS CASTRO FERREIRA
hcfmusp.description.beginpage824
hcfmusp.description.endpage832
hcfmusp.description.issue12
hcfmusp.description.volume28
hcfmusp.origemWOS
hcfmusp.origem.pubmed24316855
hcfmusp.origem.scieloSCIELO:S0102-86502013001200004
hcfmusp.origem.scopus2-s2.0-84890069613
hcfmusp.origem.wosWOS:000328649900004
hcfmusp.publisher.citySAO PAULO
hcfmusp.publisher.countryBRAZIL
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hcfmusp.remissive.sponsorshipCAPES
hcfmusp.scopus.lastupdate2024-05-10
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