Development of a Novel Large Animal Model to Evaluate Human Dental Pulp Stem Cells for Articular Cartilage Treatment

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorFERNANDES, Tiago Lazzaretti
dc.contributor.authorSHIMOMURA, Kazunori
dc.contributor.authorASPERTI, Andre
dc.contributor.authorPINHEIRO, Carla Cristina Gomes
dc.contributor.authorCAETANO, Heloisa Vasconcellos Amaral
dc.contributor.authorOLIVEIRA, Claudia Regina G. C. M.
dc.contributor.authorNAKAMURA, Norimasa
dc.contributor.authorHERNANDEZ, Arnaldo Jose
dc.contributor.authorBUENO, Daniela Franco
dc.date.accessioned2018-11-21T17:05:28Z
dc.date.available2018-11-21T17:05:28Z
dc.date.issued2018
dc.description.abstractChondral lesion is a pathology with high prevalence, reaching as much as 63% of general population and 36% among athletes. The ability of human Dental Pulp Stem Cells (DPSCs) to differentiate into chondroblasts in vitro suggests that this stem cell type may be useful for tissue bioengineering. However, we have yet to identify a study of large animal models in which DPSCs were used to repair articular cartilage. Therefore, this study aimed to describe a novel treatment for cartilage lesion with DPSCs on a large animal model. Mesenchymal stem cells (MSC) were obtained from deciduous teeth and characterized by flow cytometry. DPSCs were cultured and added to a collagen type I/III biomaterial composite scaffold. Brazilian miniature pig (BR-1) was used. A 6-mm diameter, full-thickness chondral defect was created in each posterior medial condyle. The defects were covered with scaffold alone or scaffold + DPSCs on the contralateral side. Animals were euthanized 6 weeks post-surgery. Cartilage defects were analyzed macroscopically and histology according to modified O'Driscoll scoring system. Flow cytometry confirmed characterization of DPSCs as MSCs. Macroscopic and histological findings suggested that this time period was reasonable for evaluating cartilage repair. To our knowledge, this study provides the first description of an animal model using DPSCs to study the differentiation of hyaline articular cartilage in vivo. The animals tolerated the procedure well and did not show clinical or histological rejection of the DPSCs, reinforcing the feasibility of this descriptive miniature pig model for pre-clinical studies.
dc.description.indexMEDLINE
dc.description.sponsorshipISAKOS Young Investigators Research Mentoring Program 2016
dc.identifier.citationSTEM CELL REVIEWS AND REPORTS, v.14, n.5, p.734-743, 2018
dc.identifier.doi10.1007/s12015-018-9820-2
dc.identifier.eissn1558-6804
dc.identifier.issn1550-8943
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/29522
dc.language.isoeng
dc.publisherHUMANA PRESS INC
dc.relation.ispartofStem Cell Reviews and Reports
dc.rightsopenAccess
dc.rights.holderCopyright HUMANA PRESS INC
dc.subjectMesenchymal stem cells
dc.subjectTissue engineering
dc.subjectHuman dental pulp stem cells
dc.subjectArticular cartilage
dc.subjectHyaline cartilage
dc.subjectpre-clinical study
dc.subjectlarge animal model
dc.subjectminiature pig
dc.subject.otherfull-thickness defects
dc.subject.othersubchondral bone
dc.subject.otherminipig model
dc.subject.otherrepair
dc.subject.otherdifferentiation
dc.subject.otherreconstruction
dc.subject.othergeneration
dc.subject.otherknee
dc.subject.wosCell & Tissue Engineering
dc.subject.wosCell Biology
dc.subject.wosMedicine, Research & Experimental
dc.titleDevelopment of a Novel Large Animal Model to Evaluate Human Dental Pulp Stem Cells for Articular Cartilage Treatment
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.affiliation.countryJapão
hcfmusp.affiliation.countryisojp
hcfmusp.author.externalSHIMOMURA, Kazunori:Osaka Univ, Grad Sch Med, Dept Orthoped, 2-2 Yamadaoka, Suita, Osaka 5650871, Japan
hcfmusp.author.externalPINHEIRO, Carla Cristina Gomes:Hosp Sirio Libanes, 115 Rua Dona Adma Jafet, BR-01308050 Sao Paulo, SP, Brazil
hcfmusp.author.externalCAETANO, Heloisa Vasconcellos Amaral:Hosp Sirio Libanes, 115 Rua Dona Adma Jafet, BR-01308050 Sao Paulo, SP, Brazil
hcfmusp.author.externalNAKAMURA, Norimasa:Osaka Univ, Ctr Adv Med Engn & Informat, 2-2 Yamadaoka, Suita, Osaka 5650871, Japan
hcfmusp.author.externalBUENO, Daniela Franco:Hosp Sirio Libanes, 115 Rua Dona Adma Jafet, BR-01308050 Sao Paulo, SP, Brazil
hcfmusp.citation.scopus32
hcfmusp.contributor.author-fmusphcTIAGO LAZZARETTI FERNANDES
hcfmusp.contributor.author-fmusphcANDRE MARANGONI ASPERTI
hcfmusp.contributor.author-fmusphcCLAUDIA REGINA GOMES CARDIM MENDES DE OLIVEIRA
hcfmusp.contributor.author-fmusphcARNALDO JOSE HERNANDEZ
hcfmusp.description.beginpage734
hcfmusp.description.endpage743
hcfmusp.description.issue5
hcfmusp.description.volume14
hcfmusp.origemWOS
hcfmusp.origem.pubmed29728886
hcfmusp.origem.scopus2-s2.0-85046438359
hcfmusp.origem.wosWOS:000443477500012
hcfmusp.publisher.cityTOTOWA
hcfmusp.publisher.countryUSA
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