A NEW MODEL OF LARGE-FOR-SIZE PORCINE LIVER TRANSPLANTATION WITH AORTIC CLAMPING

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorLEAL, Antonio Jose Goncalves
dc.contributor.authorBELON, Alessandro Rodrigo
dc.contributor.authorTANNURI, Ana Cristina Aoun
dc.contributor.authorGUIMARAES, Raimundo Renato Nunes
dc.contributor.authorCOELHO, Maria Cecilia Mendonca
dc.contributor.authorGONCALVES, Josiane De Oliveira
dc.contributor.authorSOKOL, Suellen Serafini
dc.contributor.authorMELO, Evandro Sobroza De
dc.contributor.authorOTOCH, Jose Pinhata
dc.contributor.authorTANNURI, Uenis
dc.date.accessioned2013-10-11T21:15:13Z
dc.date.available2013-10-11T21:15:13Z
dc.date.issued2013
dc.description.abstractOBJECTIVE: The objective of the present study was to create a swine model of large-for-size liver transplantation, without venovenous bypass but clamping of the supraceliac aorta during the hepatic phase. MATERIAL AND METHODS: Fourteen Landrace-Large white pigs (weight 17 to 20 kg) were anesthetized with continuous infusion of propofol and fentanyl, and mechanically ventilated. They underwent orthotopic liver transplantation(OLT) with whole liver grafts and were divided randomly into two experimental groups, according to donor size. Regular size group (NS-n=7): donors weight were similar to the receptors (17–20 kg). Large-for-size group (LFS-n=7): donors weight was nearly two times the receptor ′ s (40–50 kg). Blood for serum levels of aspartate aminotransferase (AST) and hepatic tissue for histological examination and quantification of Bax (a proapoptotic protein) gene expression though real time PCR were sampled from the recipient at baseline, 1 and 3 h after portal reperfusion. RESULTS: In NS group, one death was related to hemodynamic instability just after aortic release and another due to bleeding by laceration on graft surface. In LFS 1,3 group, the two deaths were associated to hemodynamic instability just after aortic release. Table 1 shows the changes in AST levels over the experiment in both groups. Table 2 presents the histological results. Table 3 shows the results of Bax gene expression for the NS and LFS groups. CONCLUSION: This large-animal model is straightforward, reproducible, and clinically relevant. It provides the appropriate size and anatomy that resemble humans for the development and practice of new surgical techniques.
dc.description.conferencedateNOV 08-09, 2012
dc.description.conferencelocalSao Paulo, BRAZIL
dc.description.conferencename1st Brazilian Symposium of Pediatric Transplantation
dc.description.indexMEDLINE
dc.identifier.citationPEDIATRIC TRANSPLANTATION, v.17, n.2, p.192-192, 2013
dc.identifier.doi10.1111/petr.12037
dc.identifier.issn1397-3142
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/2678
dc.language.isoeng
dc.publisherWILEY-BLACKWELL
dc.relation.ispartofPediatric Transplantation
dc.rightsrestrictedAccess
dc.rights.holderCopyright WILEY-BLACKWELL
dc.subject.wosPediatrics
dc.subject.wosTransplantation
dc.titleA NEW MODEL OF LARGE-FOR-SIZE PORCINE LIVER TRANSPLANTATION WITH AORTIC CLAMPING
dc.typeconferenceObject
dc.type.categorymeeting abstract
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.contributor.author-fmusphcANTONIO JOSE GONCALVES E LEAL
hcfmusp.contributor.author-fmusphcALESSANDRO RODRIGO BELON
hcfmusp.contributor.author-fmusphcANA CRISTINA AOUN TANNURI
hcfmusp.contributor.author-fmusphcRAIMUNDO RENATO NUNES GUIMARAES
hcfmusp.contributor.author-fmusphcMARIA CECILIA DE MENDONCA COELHO
hcfmusp.contributor.author-fmusphcJOSIANE DE OLIVEIRA GONCALVES
hcfmusp.contributor.author-fmusphcSUELLEN SERAFINI
hcfmusp.contributor.author-fmusphcEVANDRO SOBROZA DE MELLO
hcfmusp.contributor.author-fmusphcJOSE PINHATA OTOCH
hcfmusp.contributor.author-fmusphcUENIS TANNURI
hcfmusp.description.beginpage192
hcfmusp.description.endpage192
hcfmusp.description.issue2
hcfmusp.description.volume17
hcfmusp.origemWOS
hcfmusp.origem.wosWOS:000315467000022
hcfmusp.publisher.cityHOBOKEN
hcfmusp.publisher.countryUSA
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Comunicações em Eventos - FM/MCG
Comunicações em Eventos - FM/MPE
Comunicações em Eventos - FM/MPT
Comunicações em Eventos - HC/ICESP
Comunicações em Eventos - HC/ICHC
Comunicações em Eventos - HC/ICr
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