S-nitroso-N-acetylcysteine attenuates liver fibrosis in experimental nonalcoholic steatohepatitis
Carregando...
Citações na Scopus
23
Tipo de produção
article
Data de publicação
2013
Título da Revista
ISSN da Revista
Título do Volume
Editora
DOVE MEDICAL PRESS LTD
Autores
OLIVEIRA, Marcelo G. de
COGLIATI, Bruno
SOUZA, Gabriela F. P. de
RABELO, Fabiola
Citação
DRUG DESIGN DEVELOPMENT AND THERAPY, v.7, p.553-563, 2013
Resumo
S-Nitroso-N-acetylcysteine (SNAC) is a water soluble primary S-nitrosothiol capable of transferring and releasing nitric oxide and inducing several biochemical activities, including modulation of hepatic stellate cell activation. In this study, we evaluated the antifibrotic activity of SNAC in an animal model of nonalcoholic steatohepatitis (NASH) induced in Sprague-Dawley rats fed with a choline-deficient, high trans fat diet and exposed to diethylnitrosamine for 8 weeks. The rats were divided into three groups: SNAC, which received oral SNAC solution daily; NASH, which received the vehicle; and control, which received standard diet and vehicle. Genes related to fibrosis (matrix metalloproteinases [MMP]-13, -9, and -2), transforming growth factor beta-1 [TGF beta-1], collagen-1 alpha, and tissue inhibitors of metalloproteinase [TIMP-1 and -2] and oxidative stress (heat-shock proteins [HSP]-60 and -90) were evaluated. SNAC led to a 34.4% reduction in the collagen occupied area associated with upregulation of MMP-13 and -9 and downregulation of HSP-60, TIMP-2, TGF beta-1, and collagen-1 alpha. These results indicate that oral SNAC administration may represent a potential antifibrotic treatment for NASH.
Palavras-chave
S-nitroso-N-acetylcysteine, fibrogenesis, NASH, diethylnitrosamine
Referências
- Aitio ML, 2006, BRIT J CLIN PHARMACO, V61, P5, DOI 10.1111/j.1365-2125.2005.02523.x
- Argo CK, 2009, CLIN LIVER DIS, V13, P511, DOI 10.1016/j.cld.2009.07.005
- Baumgardner JN, 2008, J NUTR, V138, P1872
- Benyon RC, 2001, SEMIN LIVER DIS, V21, P373, DOI 10.1055/s-2001-17552
- Byrne CD, 2009, CLIN SCI, V116, P539, DOI 10.1042/CS20080253
- Cogliati Bruno, 2010, Arq Gastroenterol, V47, P79, DOI 10.1590/S0004-28032010000100014
- de Lima VM, 2008, J HEPATOL, V49, P1055, DOI 10.1016/j.jhep.2008.07.024
- de Oliveira CPMS, 2006, J HEPATOL, V45, P725, DOI 10.1016/j.jhep.2006.05.017
- de Oliveira MG, 2002, J PHYS CHEM A, V106, P8963, DOI 10.1021/jp025756u
- Ekstedt M, 2006, HEPATOLOGY, V44, P865, DOI 10.1002/hep.21327
- Friedman SL, 2008, GASTROENTEROLOGY, V134, P1655, DOI 10.1053/j.gastro.2008.03.003
- Galicia-Moreno M, 2009, EUR J GASTROEN HEPAT, V21, P908, DOI 10.1097/MEG.0b013e32831f1f3a
- Gaston B, 1999, BBA-BIOENERGETICS, V1411, P323, DOI 10.1016/S0005-2728(99)00023-7
- Hemmann S, 2007, J HEPATOL, V46, P955, DOI 10.1016/j.jhep.2007.02.003
- Hernandez-Gea V, 2011, ANNU REV PATHOL-MECH, V6, P425, DOI 10.1146/annurev-pathol-011110-130246
- Ignarro LJ, 2002, CIRC RES, V90, P21, DOI 10.1161/hh0102.102330
- Iredale JP, 1998, J CLIN INVEST, V102, P538, DOI 10.1172/JCI1018
- Issa R, 2004, GASTROENTEROLOGY, V126, P1795, DOI 10.1053/j.gastro.2004.03.009
- Issa Razao, 2003, FASEB Journal, V17, P47
- JAMES J, 1990, LIVER, V10, P1
- Knittel T, 2000, HISTOCHEM CELL BIOL, V113, P443
- Lewis JR, 2010, DIGEST DIS SCI, V55, P560, DOI 10.1007/s10620-009-1081-0
- Livak KJ, 2001, METHODS, V25, P402, DOI 10.1006/meth.2001.1262
- Martinez V, 2002, J PHARMACOL EXP THER, V301, P611, DOI 10.1124/jpet.301.2.611
- Okazaki Isao, 2001, Keio Journal of Medicine, V50, P58
- Oliveira CP, 2006, WORLD J GASTROENTERO, V12, P1905
- Oliveira CP, 2008, J AM COLL NUTR, V27, P299
- Oliveira CPMS, 2007, BIOCHEM PHARMACOL, V74, P290, DOI 10.1016/j.bcp.2007.04.013
- Ramachandran P, 2009, ANN HEPATOL, V8, P283
- Ricardo KFS, 2002, NITRIC OXIDE-BIOL CH, V7, P57, DOI 10.1016/S1089-8603(02)00009-5
- Roderfeld M, 2007, Z GASTROENTEROL, V45, P25, DOI 10.1055/s-2006-927388
- Roderfeld M, 2006, FASEB J, V20, P444, DOI 10.1096/fj.05-4828com
- RUBBO H, 1994, J BIOL CHEM, V269, P26066
- Sherman M, 2010, SEMIN LIVER DIS, V30, P3, DOI 10.1055/s-0030-1247128
- Simplicio FI, 2010, J BRAZIL CHEM SOC, V21, P1885, DOI 10.1590/S0103-50532010001000013
- Stefano JT, 2011, NITRIC OXIDE-BIOL CH, V25, P360, DOI 10.1016/j.niox.2011.07.001
- Timerghazin QK, 2007, ORG LETT, V9, P3049, DOI 10.1021/ol0711016
- Ueberham E, 2003, HEPATOLOGY, V37, P1067, DOI 10.1053/jhep.2003.50196
- Vercelino R, 2010, J MOL MED-JMM, V88, P401, DOI 10.1007/s00109-009-0577-6
- Wang ZP, 2000, J BIOL CHEM, V275, P26411, DOI 10.1074/jbc.M001270200
- Williams DLH, 2003, ORG BIOMOL CHEM, V1, P441, DOI 10.1039/b209748f
- Zhou XY, 2004, J BIOL CHEM, V279, P23996, DOI 10.1074/jbc.M311668200