Sensitization Prevalence, Antibody Cross-Reactivity and Immunogenic Peptide Profile of Api g 2, the Non-Specific Lipid Transfer Protein 1 of Celery

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorGADERMAIER, Gabriele
dc.contributor.authorHAUSER, Michael
dc.contributor.authorEGGER, Matthias
dc.contributor.authorFERRARA, Rosetta
dc.contributor.authorBRIZA, Peter
dc.contributor.authorSANTOS, Keity Souza
dc.contributor.authorZENNARO, Danila
dc.contributor.authorGIRBL, Tamara
dc.contributor.authorZUIDMEER-JONGEJAN, Laurian
dc.contributor.authorMARI, Adriano
dc.contributor.authorFERREIRA, Fatima
dc.date.accessioned2017-11-27T16:36:08Z
dc.date.available2017-11-27T16:36:08Z
dc.date.issued2011
dc.description.abstractBackground: Celery (Apium graveolens) represents a relevant allergen source that can elicit severe reactions in the adult population. To investigate the sensitization prevalence and cross-reactivity of Api g 2 from celery stalks in a Mediterranean population and in a mouse model. Methodology: 786 non-randomized subjects from Italy were screened for IgE reactivity to rApi g 2, rArt v 3 (mugwort pollen LTP) and nPru p 3 (peach LTP) using an allergen microarray. Clinical data of 32 selected patients with reactivity to LTP under investigation were evaluated. Specific IgE titers and cross-inhibitions were performed in ELISA and allergen microarray. Balb/c mice were immunized with purified LTPs; IgG titers were determined in ELISA and mediator release was examined using RBL-2H3 cells. Simulated endolysosomal digestion was performed using microsomes obtained from human DCs. Results: IgE testing showed a sensitization prevalence of 25.6% to Api g 2, 18.6% to Art v 3, and 28.6% to Pru p 3 and frequent co-sensitization and correlating IgE-reactivity was observed. 10/32 patients suffering from LTP-related allergy reported symptoms upon consumption of celery stalks which mainly presented as OAS. Considerable IgE cross-reactivity was observed between Api g 2, Art v 3, and Pru p 3 with varying inhibition degrees of individual patients' sera. Simulating LTP mono-sensitization in a mouse model showed development of more congruent antibody specificities between Api g 2 and Art v 3. Notably, biologically relevant murine IgE cross-reactivity was restricted to the latter and diverse from Pru p 3 epitopes. Endolysosomal processing of LTP showed generation of similar clusters, which presumably represent T-cell peptides. Conclusions: Api g 2 represents a relevant celery stalk allergen in the LTP-sensitized population. The molecule displays common B cell epitopes and endolysosomal peptides that encompass T cell epitopes with pollen and plant-food derived LTP.
dc.description.indexMEDLINE
dc.description.sponsorshipChristian-Doppler Research Association, Biomay AG, Vienna, Austria
dc.description.sponsorshipItalian Ministry of Health
dc.identifier.citationPLOS ONE, v.6, n.8, article ID e24150, 11p, 2011
dc.identifier.doi10.1371/journal.pone.0024150
dc.identifier.issn1932-6203
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/23556
dc.language.isoeng
dc.publisherPUBLIC LIBRARY SCIENCE
dc.relation.ispartofPlos One
dc.rightsopenAccess
dc.rights.holderCopyright PUBLIC LIBRARY SCIENCE
dc.subject.otherpru p 3
dc.subject.othermajor peach allergen
dc.subject.othercomponent-resolved diagnosis
dc.subject.othercontrolled food challenge
dc.subject.othert-cell response
dc.subject.otherdouble-blind
dc.subject.otherplant foods
dc.subject.otherige-binding
dc.subject.otherclinical expression
dc.subject.othermugwort pollen
dc.subject.wosMultidisciplinary Sciences
dc.titleSensitization Prevalence, Antibody Cross-Reactivity and Immunogenic Peptide Profile of Api g 2, the Non-Specific Lipid Transfer Protein 1 of Celery
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.affiliation.countryHolanda
hcfmusp.affiliation.countryItália
hcfmusp.affiliation.countryÁustria
hcfmusp.affiliation.countryisoat
hcfmusp.affiliation.countryisoit
hcfmusp.affiliation.countryisonl
hcfmusp.author.externalGADERMAIER, Gabriele:Salzburg Univ, Christian Doppler Lab Allergy Diag & Therapy, A-5020 Salzburg, Austria
hcfmusp.author.externalHAUSER, Michael:Salzburg Univ, Christian Doppler Lab Allergy Diag & Therapy, A-5020 Salzburg, Austria
hcfmusp.author.externalEGGER, Matthias:Salzburg Univ, Christian Doppler Lab Allergy Diag & Therapy, A-5020 Salzburg, Austria
hcfmusp.author.externalFERRARA, Rosetta:IDI IRCCS, Ctr Mol Allergol, Rome, Italy
hcfmusp.author.externalBRIZA, Peter:Salzburg Univ, Christian Doppler Lab Allergy Diag & Therapy, A-5020 Salzburg, Austria
hcfmusp.author.externalZENNARO, Danila:IDI IRCCS, Ctr Mol Allergol, Rome, Italy
hcfmusp.author.externalGIRBL, Tamara:Salzburg Univ, Christian Doppler Lab Allergy Diag & Therapy, A-5020 Salzburg, Austria
hcfmusp.author.externalZUIDMEER-JONGEJAN, Laurian:Univ Amsterdam, Acad Med Ctr, Lab Allergy, Dept Expt Immunol, NL-1105 AZ Amsterdam, Netherlands
hcfmusp.author.externalMARI, Adriano:IDI IRCCS, Ctr Mol Allergol, Rome, Italy
hcfmusp.author.externalFERREIRA, Fatima:Salzburg Univ, Christian Doppler Lab Allergy Diag & Therapy, A-5020 Salzburg, Austria
hcfmusp.citation.scopus53
hcfmusp.contributor.author-fmusphcKEITY SOUZA SANTOS
hcfmusp.description.articlenumbere24150
hcfmusp.description.issue8
hcfmusp.description.volume6
hcfmusp.origemWOS
hcfmusp.origem.pubmed21897872
hcfmusp.origem.pubmed21897872
hcfmusp.origem.scopus2-s2.0-80052288824
hcfmusp.origem.wosWOS:000294676900037
hcfmusp.publisher.citySAN FRANCISCO
hcfmusp.publisher.countryUSA
hcfmusp.relation.referenceGarcia-Casado G, 2003, J ALLERGY CLIN IMMUN, V112, P599, DOI 10.1037/mai.2003.1638
hcfmusp.relation.referenceEgger M, 2006, ALLERGY, V61, P461, DOI 10.1111/j.1398-9995.2006.00994.x
hcfmusp.relation.referenceTordesillas L, 2009, MOL IMMUNOL, V46, P722, DOI 10.1016/j.molimm.2008.10.018
hcfmusp.relation.referencePastorello EA, 2010, INT ARCH ALLERGY IMM, V153, P1, DOI 10.1159/000301573
hcfmusp.relation.referenceBorges JP, 2008, BIOCHEM BIOPH RES CO, V365, P685, DOI 10.1016/j.bbrc.2007.11.046
hcfmusp.relation.referenceAsero R, 2009, CLIN EXP ALLERGY, V39, P547, DOI 10.1111/j.1365-2222.2008.03167.x
hcfmusp.relation.referenceHochreiter R, 2003, EUR J IMMUNOL, V33, P1667, DOI 10.1002/eji.200323377
hcfmusp.relation.referencePacios LF, 2008, MOL IMMUNOL, V45, P2269, DOI 10.1016/j.molimm.2007.11.022
hcfmusp.relation.referenceEgger M, 2010, CURR ALLERGY ASTHM R, V10, P326, DOI 10.1007/s11882-010-0128-9
hcfmusp.relation.referencePalacin A, 2006, J ALLERGY CLIN IMMUN, V117, P1423, DOI 10.1016/j.jaci.2006.01.026
hcfmusp.relation.referenceCiardiello MA, 2010, ALLERGY, V65, P597, DOI 10.1111/j.1398-9995.2009.02277.x
hcfmusp.relation.referenceAsero R, 2009, INT ARCH ALLERGY IMM, V150, P271, DOI 10.1159/000222679
hcfmusp.relation.referenceScala E, 2010, CLIN EXP ALLERGY, V40, P911, DOI 10.1111/j.1365-2222.2010.03470.x
hcfmusp.relation.referenceRuckrich T, 2006, BIOL CHEM, V387, P1503, DOI 10.1515/BC.2006.188
hcfmusp.relation.referenceGadermaier G, 2009, MOL IMMUNOL, V46, P1919, DOI 10.1016/j.molimm.2009.03.021
hcfmusp.relation.referenceBREITENEDER H, 1995, EUR J BIOCHEM, V233, P484, DOI 10.1111/j.1432-1033.1995.484_2.x
hcfmusp.relation.referenceAsero R, 2006, CLIN EXP ALLERGY, V36, P658, DOI 10.1111/j.1365-2222.2006.02477.x
hcfmusp.relation.referenceBarber D, 2009, CLIN EXP ALLERGY, V39, P1764, DOI 10.1111/j.1365-2222.2009.03351.x
hcfmusp.relation.referenceBallmer-Weber BK, 2000, J ALLERGY CLIN IMMUN, V106, P373, DOI 10.1067/mai.2000.107196
hcfmusp.relation.referenceBallmer-Weber BK, 2002, ALLERGY, V57, P228, DOI 10.1034/j.1398-9995.2002.1o3319.x
hcfmusp.relation.referenceBauermeister K, 2009, J ALLERGY CLIN IMMUN, V124, P1273, DOI 10.1016/j.jaci.2009.07.033
hcfmusp.relation.referenceBublin M, 2003, FASEB J, V17, P1697, DOI 10.1096/fj.02-0872fje
hcfmusp.relation.referenceBublin M, 2008, MOL NUTR FOOD RES, V52, pS241, DOI 10.1002/mnfr.200700270
hcfmusp.relation.referenceCAVATORTA V, 2010, MOL NUTR FOOD RES
hcfmusp.relation.referenceEgger M, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0017278
hcfmusp.relation.referenceFernandez-Rivas M, 2006, J ALLERGY CLIN IMMUN, V118, P481, DOI 10.1016/j.jaci.2006.05.012
hcfmusp.relation.referenceFigueroa J, 2005, ALLERGY, V60, P48, DOI 10.1111/j.1398-9995.2005.00644.x
hcfmusp.relation.referenceFotisch K, 1999, INT ARCH ALLERGY IMM, V120, P30, DOI 10.1159/000024217
hcfmusp.relation.referenceGadermaier G, 2011, MOL NUTR FOOD RES, V55, P568, DOI 10.1002/mnfr.201000443
hcfmusp.relation.referenceGaier S, 2008, MOL NUTR FOOD RES, V52, pS220, DOI 10.1002/mnfr.200700274
hcfmusp.relation.referenceGarcia-Selles FJ, 2002, INT ARCH ALLERGY IMM, V128, P115, DOI 10.1159/000059401
hcfmusp.relation.referenceHoffmann-Sommergruber K, 1999, J ALLERGY CLIN IMMUN, V104, P478, DOI 10.1016/S0091-6749(99)70397-0
hcfmusp.relation.referenceJankiewicz A, 1996, INT ARCH ALLERGY IMM, V111, P268
hcfmusp.relation.referenceLombardero M, 2004, CLIN EXP ALLERGY, V34, P1415, DOI 10.1111/j.1365-2222.2004.02053.x
hcfmusp.relation.referenceLuttkopf D, 2000, J ALLERGY CLIN IMMUN, V106, P390, DOI 10.1067/mai.2000.108711
hcfmusp.relation.referenceMUTSCHLECHNER S, 2010, J ALLERGY CLIN IMMUN
hcfmusp.relation.referencePastorello EA, 2002, J ALLERGY CLIN IMMUN, V110, P310, DOI 10.1067/mai.2002.125830
hcfmusp.relation.referenceScheurer S, 2000, CLIN EXP ALLERGY, V30, P962, DOI 10.1046/j.1365-2222.2000.00821.x
hcfmusp.relation.referenceSchulten V, 2011, ALLERGY, V66, P1005, DOI 10.1111/j.1398-9995.2011.02567.x
hcfmusp.relation.referenceSchulten V, 2009, J ALLERGY CLIN IMMUN, V124, P100, DOI 10.1016/j.jaci.2009.02.010
hcfmusp.relation.referenceToda M., 2011, J ALLERGY CLIN IMMUN
hcfmusp.relation.referenceVALLIER P, 1992, CLIN EXP ALLERGY, V22, P774, DOI 10.1111/j.1365-2222.1992.tb02818.x
hcfmusp.relation.referenceWijesinha-Bettoni R, 2010, BIOCHEMISTRY-US, V49, P2130, DOI 10.1021/bi901939z
hcfmusp.relation.referenceZuidmeer L, 2007, CURR OPIN ALLERGY CL, V7, P269, DOI 10.1097/ACI.0b013e32814a5401
hcfmusp.relation.referenceZuidmeer L, 2005, INT ARCH ALLERGY IMM, V137, P273, DOI 10.1159/000086420
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