Virulence and resistance profiles of MRSA isolates in pre- and post-liver transplantation patients using microarray

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art_HEIJDEN_Virulence_and_resistance_profiles_of_MRSA_isolates_in_2016.PDF (677.4 KB)
Citações na Scopus
7
Tipo de produção
article
Data de publicação
2016
DOI
Scopus
Web of Science
PubMed
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Título do Volume
Editora
MICROBIOLOGY SOC
Autores
Citação
JOURNAL OF MEDICAL MICROBIOLOGY, v.65, p.1060-1073, 2016
Projetos de Pesquisa
Unidades Organizacionais
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Resumo
Methicillin-resistant Staphylococcus aureus (MRSA) screening plays a great role in preventing infections in surgical patients. This study aims to evaluate clonality, virulence and resistance of MRSA in pre- and post-liver transplantation (LT) patients. Nasal and groin swabs of 190 patients were collected. PCR for virulence genes and staphylococcal cassette chromosome mec (SCCmec) types, microarray, PFGE, multilocus sequence typing and MIC were performed. MRSA carriers were detected in 20.5% (39/190) of the patients. However, only three colonized patients developed infections post-LT. Sixty-nine MRSA isolates were identified, and the most frequent SCCmec type was type II (29/69; 42.0 %). Most isolates (57/69; 82.6 %) were susceptible to trimethoprim-sulfamethoxazole (TMP/SMX) and harboured the lukD, lukE, clf and fnbA genes as determined by PCR. Five sequence types (ST) were identified among nine clones; 36.2% (25/69) isolates belonged to a predominant clone (ST105 and SCCmec type II) that was susceptible to TMP/SMX, mupirocin and chlorhexidine, which had 87.9% similarity with the New York/Japan clone. The array showed virulence difference in isolates of the same clone and patients and that colonized isolates (pre-LT patients) were less virulent than those post-LT and those infected. Therefore, despite the high frequency of MRSA colonization, infection due to MRSA was uncommon in our LT unit. MRSA isolates presented great diversity. Isolates of the same clone expressed different virulence factors by array. Colonizing isolates pre-LT expressed less virulent factors than post-LT and infecting isolates.
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https://observatorio.fm.usp.br/handle/OPI/17085
Referências
  1. Baba-Moussa L, 2008, J HOSP INFECT, V68, P32, DOI 10.1016/j.jhin.2007.10.010
  2. Bert F, 2010, LIVER TRANSPLANT, V16, P393, DOI 10.1002/lt.21991
  3. Bocher S, 2008, J CLIN MICROBIOL, V46, P3136, DOI 10.1128/JCM.00478-08
  4. Caiaffa HH, 2013, DIAGN MICR INFEC DIS, V76, P518, DOI 10.1016/j.diagmicrobio.2013.04.024
  5. Centers for Disease Control and Prevention, 2002, MMWR-MORBID MORTAL W, V51, P565
  6. Chini V, 2007, LETT APPL MICROBIOL, V45, P479, DOI 10.1111/j.1472-765X.2007.02208.x
  7. CLSI, 2014, M100S24 CLSI
  8. CLSI, 2009, METH DIL ANT SUSC TE
  9. de Oliveira LM, 2015, BMC MICROBIOL, V15, DOI 10.1186/s12866-015-0598-y
  10. Desai D, 2003, LIVER TRANSPLANT, V9, P754, DOI 10.1053/jlts.2003.50142
  11. Enright MC, 2000, J CLIN MICROBIOL, V38, P1008
  12. GARNER JS, 1988, AM J INFECT CONTROL, V16, P128, DOI 10.1016/0196-6553(88)90053-3
  13. Hashimoto M, 2007, TRANSPL P, V39, P3271, DOI 10.1016/j.transproceed.2007.09.035
  14. Hashimoto M, 2008, TRANSPL INFECT DIS, V10, P110, DOI 10.1111/j.1399-3062.2007.00253.x
  15. Ito T, 2004, ANTIMICROB AGENTS CH, V48, P2637, DOI 10.1128/AAC.48.7.2637-2651.2004
  16. Jensen SO, 2009, FUTURE MICROBIOL, V4, P565, DOI [10.2217/fmb.09.30, 10.2217/FMB.09.30]
  17. Kateete D. P., 2010, ANN CLIN MICROB ANTI, V13, P9
  18. Kaur Dardi Charan, 2014, Indian J Crit Care Med, V18, P716, DOI 10.4103/0972-5229.144013
  19. Kearns AM, 1999, J HOSP INFECT, V43, P33, DOI 10.1053/jhin.1999.0631
  20. Kim YJ, 2015, KOREAN J INTERN MED, V30, P694, DOI 10.3904/kjim.2015.30.5.694
  21. Leelaporn A, 1996, J BACTERIOL, V178, P6070
  22. Li HA, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0053341
  23. Malachowa N, 2010, CELL MOL LIFE SCI, V67, P3057, DOI 10.1007/s00018-010-0389-4
  24. Malaviolle X, 2008, J ANTIMICROB CHEMOTH, V62, P1018, DOI 10.1093/jac/dkn345
  25. McDanel JS, 2013, ANTIMICROB AGENTS CH, V57, P552, DOI [10.1128/AAC.01623-12, 10.1128/AAC.01623]
  26. McDougal LK, 2003, J CLIN MICROBIOL, V41, P5113, DOI 10.1128/JCM.41.11.5113-5120.2003
  27. McNicholas S, 2011, J CLIN MICROBIOL, V49, P4349, DOI 10.1128/JCM.05017-11
  28. Mulvey MR, 2001, J CLIN MICROBIOL, V39, P3481, DOI 10.1128/JCM.39.10.3481-3485.2001
  29. Okuma K, 2002, J CLIN MICROBIOL, V40, P4289, DOI 10.1128/JCM.40.11.4289-4294.2002
  30. Oliveira DC, 2002, ANTIMICROB AGENTS CH, V46, P2155, DOI 10.1128/AAC.46.7.2155-2161.2002
  31. Pacheco RL, 2011, CLINICS, V66, P2071, DOI 10.1590/S1807-59322011001200012
  32. Paterson DL, 2003, TRANSPLANTATION, V75, P194, DOI 10.1097/01.TP.0000040602.01701.85
  33. Romanelli RMC, 2010, BRAZ J INFECT DIS, V14, P54, DOI 10.1590/S1413-86702010000100011
  34. Santoro-Lopes G, 2005, LIVER TRANSPLANT, V11, P203, DOI 10.1002/lt.20338
  35. Schlievert PM, 2010, J ALLERGY CLIN IMMUN, V125, P39, DOI 10.1016/j.jaci.2009.10.039
  36. Singh N, 2006, INFECT CONT HOSP EP, V27, P122, DOI 10.1086/500651
  37. Takatsuki M, 2010, J HEPATO-BIL-PAN SCI, V17, P839, DOI 10.1007/s00534-010-0273-5
  38. Truong-Bolduc QC, 2005, J BACTERIOL, V187, P2395, DOI 10.1128/JB.187.7.2395-2405.2005
  39. Upton A, 2003, J ANTIMICROB CHEMOTH, V51, P613, DOI 10.1093/jac/dkg127
  40. Zhang KY, 2005, J CLIN MICROBIOL, V43, P5026, DOI 10.1128/JCM.43.10.5026-5033.2005

Coleções
Artigos e Materiais de Revistas Científicas - FM/MIP
Artigos e Materiais de Revistas Científicas - FM/MGT
Artigos e Materiais de Revistas Científicas - HC/ICESP
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/03
Artigos e Materiais de Revistas Científicas - LIM/37
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