Evaluation of Downstream Regulatory Element Antagonistic Modulator Gene in Human Multinodular Goiter

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorSHINZATO, Amanda
dc.contributor.authorLERARIO, Antonio M.
dc.contributor.authorLIN, Chin J.
dc.contributor.authorDANILOVIC, Debora S.
dc.contributor.authorMARUI, Suemi
dc.contributor.authorTRARBACH, Ericka B.
dc.date.accessioned2017-06-09T15:37:39Z
dc.date.available2017-06-09T15:37:39Z
dc.date.issued2015
dc.description.abstractBackground: DREAM (Downstream Regulatory Element Antagonistic Modulator) is a neuronal calcium sensor that was suggested to modulate TSH receptor activity and whose overexpression provokes an enlargement of the thyroid gland in transgenic mice. The aim of this study was to investigate somatic mutations and DREAM gene expression in human multinodular goiter (MNG). Material/Methods: DNA and RNA samples were obtained from hyperplastic thyroid glands of 60 patients (54 females) with benign MNG. DREAM mutations were evaluated by PCR and direct automatic sequencing, whereas relative quantification of mRNA was performed by real-time PCR. Over-and under-expression were defined as a 2-fold increase and decrease in comparison to normal thyroid tissue, respectively. RQ M (relative quantification mean); SD (standard deviation). Results: DREAM expression was detected in all nodules evaluated. DREAM mRNA was overexpressed in 31.7% of MNG (RQ M=6.26; SD=5.08), whereas 53.3% and 15% had either normal (RQ M=1.16; SD=0.46) or underexpression (RQ M=0.30; SD=0.10), respectively. Regarding DREAM mutations analysis, only previously described intronic polymorphisms were observed. Conclusions: We report DREAM gene expression in the hyperplastic thyroid gland of MNG patients. However, DREAM expression did not vary significantly, and was somewhat underexpressed in most patients, suggesting that DREAM upregulation does not significantly affect nodular development in human goiter.
dc.description.indexMEDLINE
dc.identifier.citationMEDICAL SCIENCE MONITOR BASIC RESEARCH, v.21, p.179-182, 2015
dc.identifier.doi10.12659/MSMBR.895096
dc.identifier.eissn2325-4394
dc.identifier.issn2325-4416
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/20177
dc.language.isoeng
dc.publisherINT SCIENTIFIC LITERATURE, INC
dc.relation.ispartofMedical Science Monitor Basic Research
dc.rightsrestrictedAccess
dc.rights.holderCopyright INT SCIENTIFIC LITERATURE, INC
dc.subjectDNA Mutational Analysis
dc.subjectGene Expression
dc.subjectGoiter, Nodular
dc.subjectKv Channel-Interacting Proteins
dc.subject.othertranscriptional repressor dream
dc.subject.otherexpression
dc.subject.wosMedicine, Research & Experimental
dc.titleEvaluation of Downstream Regulatory Element Antagonistic Modulator Gene in Human Multinodular Goiter
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.citation.scopus1
hcfmusp.contributor.author-fmusphcAMANDA SHINZATO
hcfmusp.contributor.author-fmusphcANTONIO MARCONDES LERARIO
hcfmusp.contributor.author-fmusphcCHIN JIA LIN
hcfmusp.contributor.author-fmusphcDEBORA LUCIA SEGURO DANILOVIC
hcfmusp.contributor.author-fmusphcSUEMI MARUI
hcfmusp.contributor.author-fmusphcERICKA BARBOSA TRARBACH
hcfmusp.description.beginpage179
hcfmusp.description.endpage182
hcfmusp.description.volume21
hcfmusp.origemWOS
hcfmusp.origem.pubmed26319784
hcfmusp.origem.scopus2-s2.0-85021388999
hcfmusp.origem.wosWOS:000399157000029
hcfmusp.publisher.citySMITHTOWN
hcfmusp.publisher.countryUSA
hcfmusp.relation.referenceBaczyk D, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0051837
hcfmusp.relation.referenceBrix TH, 1999, J CLIN ENDOCR METAB, V84, P3071, DOI 10.1210/jc.84.9.3071
hcfmusp.relation.referenceCarrion AM, 1999, NATURE, V398, P80
hcfmusp.relation.referenceD'Andrea B, 2005, EXP CELL RES, V305, P166, DOI 10.1016/j.yexcr.2004.12.012
hcfmusp.relation.referenceDerwahl M, 1998, THYROID CANC, P155
hcfmusp.relation.referenceKrohn K, 2005, ENDOCR REV, V26, P504, DOI 10.1210/er.2004-0005
hcfmusp.relation.referenceLedo F, 2002, EMBO J, V21, P4583, DOI 10.1093/emboj/cdf440
hcfmusp.relation.referenceLedo F, 2000, MOL CELL BIOL, V20, P9120, DOI 10.1128/MCB.20.24.9120-9126.2000
hcfmusp.relation.referenceLivak KJ, 2001, METHODS, V25, P402, DOI 10.1006/meth.2001.1262
hcfmusp.relation.referenceMatsuda M, 2006, ENDOCRINOLOGY, V147, P4608, DOI 10.1210/en.2006-0254
hcfmusp.relation.referenceMedeiros-Neto G, 2000, ENDOTEXT, P1
hcfmusp.relation.referencePinchera A., 1996, Annali Italiani di Chirurgia, V67, P317
hcfmusp.relation.referenceRivas M, 2004, J BIOL CHEM, V279, P33114, DOI 10.1074/jbc.M403526200
hcfmusp.relation.referenceRivas M, 2009, MOL ENDOCRINOL, V23, P862, DOI 10.1210/me.2008-0466
hcfmusp.relation.referenceSavignac M, 2005, EMBO J, V24, P3555, DOI 10.1038/sj.emboj.7600810
hcfmusp.relation.referenceUntergasser A, 2012, NUCLEIC ACIDS RES, V40, DOI 10.1093/nar/gks596
hcfmusp.scopus.lastupdate2024-04-12
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