Immunodominant antibody responses directed to SARS-CoV-2 hotspot mutation sites and risk of immune escape

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorOLIVEIRA, Jamille Ramos
dc.contributor.authorRUIZ, Cesar Manuel Remuzgo
dc.contributor.authorMACHADO, Rafael Rahal Guaragna
dc.contributor.authorMAGAWA, Jhosiene Yukari
dc.contributor.authorDAHER, Isabela Pazotti
dc.contributor.authorURBANSKI, Alysson Henrique
dc.contributor.authorSCHMITZ, Gabriela Justamante Haendel
dc.contributor.authorARCURI, Helen Andrade
dc.contributor.authorFERREIRA, Marcelo Alves
dc.contributor.authorSASAHARA, Greyce Luri
dc.contributor.authorMEDEIROS, Giuliana Xavier de
dc.contributor.authorJR, Roberto Carlos Vieira Silva
dc.contributor.authorDURIGON, Edison Luiz
dc.contributor.authorBOSCARDIN, Silvia Beatriz
dc.contributor.authorROSA, Daniela Santoro
dc.contributor.authorSCHECHTMAN, Deborah
dc.contributor.authorNAKAYA, Helder. I. I.
dc.contributor.authorCUNHA-NETO, Edecio
dc.contributor.authorGADERMAIER, Gabriele
dc.contributor.authorKALIL, Jorge
dc.contributor.authorCOELHO, Veronica
dc.contributor.authorSANTOS, Keity Souza
dc.contributor.groupauthorCovid 19 SP Brazil Team
dc.date.accessioned2023-04-14T17:21:54Z
dc.date.available2023-04-14T17:21:54Z
dc.date.issued2023
dc.description.abstractIntroductionConsidering the likely need for the development of novel effective vaccines adapted to emerging relevant CoV-2 variants, the increasing knowledge of epitope recognition profile among convalescents and afterwards vaccinated with identification of immunodominant regions may provide important information. MethodsWe used an RBD peptide microarray to identify IgG and IgA binding regions in serum of 71 COVID-19 convalescents and 18 vaccinated individuals. ResultsWe found a set of immunodominant RBD antibody epitopes, each recognized by more than 30% of the tested cohort, that differ among the two different groups and are within conserved regions among betacoronavirus. Of those, only one peptide, P44 (S415-429), recognized by 68% of convalescents, presented IgG and IgA antibody reactivity that positively correlated with nAb titers, suggesting that this is a relevant RBD region and a potential target of IgG/IgA neutralizing activity. DiscussionThis peptide is localized within the area of contact with ACE-2 and harbors the mutation hotspot site K417 present in gamma (K417T), beta (K417N), and omicron (K417N) variants of concern. The epitope profile of vaccinated individuals differed from convalescents, with a more diverse repertoire of immunodominant peptides, recognized by more than 30% of the cohort. Noteworthy, immunodominant regions of recognition by vaccinated coincide with mutation sites at Omicron BA.1, an important variant emerging after massive vaccination. Together, our data show that immune pressure induced by dominant antibody responses may favor hotspot mutation sites and the selection of variants capable of evading humoral response.eng
dc.description.indexMEDLINE
dc.description.indexPubMed
dc.description.indexWoS
dc.description.indexScopus
dc.identifier.citationFRONTIERS IN IMMUNOLOGY, v.13, article ID 1010105, 15p, 2023
dc.identifier.doi10.3389/fimmu.2022.1010105
dc.identifier.issn1664-3224
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/52850
dc.language.isoeng
dc.publisherFRONTIERS MEDIA SAeng
dc.relation.ispartofFrontiers in Immunology
dc.rightsopenAccesseng
dc.rights.holderCopyright FRONTIERS MEDIA SAeng
dc.subjectlinear antibody epitopeseng
dc.subjectpeptide arrayeng
dc.subjectRBDeng
dc.subjectimmune pressureeng
dc.subjectsarbecoviruseng
dc.subjectbetacoronaviruseng
dc.subject.otherneutralizing antibodyeng
dc.subject.otherreceptor-bindingeng
dc.subject.wosImmunologyeng
dc.titleImmunodominant antibody responses directed to SARS-CoV-2 hotspot mutation sites and risk of immune escapeeng
dc.typearticleeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
dspace.entity.typePublication
hcfmusp.author.externalMACHADO, Rafael Rahal Guaragna:Univ Sao Paulo, Dept Microbiol, Inst Ciencias Biomed, Sao Paulo, SP, Brazil
hcfmusp.author.externalURBANSKI, Alysson Henrique:Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, Sao Paulo, SP, Brazil
hcfmusp.author.externalARCURI, Helen Andrade:Univ Estadual Paulista, Ctr Estudos Insetos Sociais, Dept Biol, Inst Biociencias Rio Claro, Rio Claro, SP, Brazil
hcfmusp.author.externalDURIGON, Edison Luiz:Univ Sao Paulo, Dept Microbiol, Inst Ciencias Biomed, Sao Paulo, SP, Brazil; Plataforma Cient Pasteur USP, Sao Paulo, SP, Brazil
hcfmusp.author.externalBOSCARDIN, Silvia Beatriz:Univ Sao Paulo, Dept Parasitol, Inst Ciencias Biomed, Sao Paulo, SP, Brazil
hcfmusp.author.externalROSA, Daniela Santoro:Univ Sao Paulo, HCFMUSP Sao Paulo, Hosp Clin,Fac Med, Inst Coracao InCor,Lab Imunol LIM19, Sao Paulo, Brazil; Univ Fed Sao Paulo UNIFESP EPM, Dept Microbiol Imunol & Parasitol, Sao Paulo, SP, Brazil
hcfmusp.author.externalSCHECHTMAN, Deborah:Univ Sao Paulo, Dept Bioquim, Inst Quim, Sao Paulo, SP, Brazil
hcfmusp.author.externalNAKAYA, Helder. I. I.:Plataforma Cient Pasteur USP, Sao Paulo, SP, Brazil; Hosp Israelita Albert Einstein, Sao Paulo, SP, Brazil
hcfmusp.author.externalGADERMAIER, Gabriele:Paris Lodron Univ Salzburg, Dept Biosci & Med Biol, Salzburg, Brazil
hcfmusp.citation.scopus5
hcfmusp.contributor.author-fmusphcJAMILLE RAMOS DE OLIVEIRA
hcfmusp.contributor.author-fmusphcCESAR MANUEL REMUZGO RUIZ
hcfmusp.contributor.author-fmusphcJHOSIENE YUKARI MAGAWA
hcfmusp.contributor.author-fmusphcISABELA PAZOTTI DAHER
hcfmusp.contributor.author-fmusphcGABRIELA JUSTAMANTE HANDEL SCHMITZ
hcfmusp.contributor.author-fmusphcMARCELO ALVES FERREIRA
hcfmusp.contributor.author-fmusphcGREYCE LURI SASAHARA
hcfmusp.contributor.author-fmusphcGIULIANA XAVIER DE MEDEIROS
hcfmusp.contributor.author-fmusphcROBERTO CARLOS VIEIRA DA SILVA JUNIOR
hcfmusp.contributor.author-fmusphcEDECIO CUNHA NETO
hcfmusp.contributor.author-fmusphcJORGE ELIAS KALIL FILHO
hcfmusp.contributor.author-fmusphcVERONICA PORTO CARREIRO DE VASCONCELLOS COELHO
hcfmusp.contributor.author-fmusphcKEITY SOUZA SANTOS
hcfmusp.description.articlenumber1010105
hcfmusp.description.volume13
hcfmusp.origemWOS
hcfmusp.origem.pubmed36685521
hcfmusp.origem.scopus2-s2.0-85146594091
hcfmusp.origem.wosWOS:000913464000001
hcfmusp.publisher.cityLAUSANNEeng
hcfmusp.publisher.countrySWITZERLANDeng
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