Harvest Technique Does Affect the Quality of Osteochondral Grafts: Histologic Evaluation Comparing Commercial Standards versus Scalpel Blade Technique

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorMERKELY, Gergo
dc.contributor.authorLEITE, Chilan B. G.
dc.contributor.authorFARINA, Evan M.
dc.contributor.authorGOMOLL, Andreas H.
dc.contributor.authorLATTERMANN, Christian
dc.date.accessioned2023-08-16T18:03:23Z
dc.date.available2023-08-16T18:03:23Z
dc.date.issued2023
dc.description.abstractObjective While the percentage of viable cells is a major determinant of graft performance during osteochondral allograft (OCA) transplantation, the baseline chondrocyte viability at the periphery of osteochondral plugs is defined at the time of harvest. In this laboratory study, we aimed to determine the optimal technique for OCA plug harvest by evaluating commercial standard techniques compared to sharp blade harvest technique. Design Osteochondral explants were harvested from bovine and human samples using 3 different techniques: (1) standard OATS manual punch device (Osteochondral Autograft Transplant System OATS; Arthrex, Naples, FL), (2) powered trephine device, and (3) fresh scalpel blade. Chondrocyte viability and the dead area at the periphery of the tissue were evaluated by LIVE/DEAD staining. Safranin-O and fast-green were performed for structural evaluation. Results For both bovine and human samples, the dead area at the periphery of the explant was significantly smaller after scalpel blade preparation compared to harvest with OATS (P < 0.001) and powered trephine devices (P < 0.001). In addition, while powered device had a smaller remaining dead area compared to the OATS device (P < 0.001), there was significantly greater tissue loss and peripheral contour change for plugs harvested with the powered trephine device. Conclusion Our study demonstrated that OCA plugs harvested with OATS and powered device lead to a significant mechanical injury at the periphery of the explants compared to a scalpel. We propose that the optimal technique for OCA harvest utilizes a combined approach incorporating a scalpel blade/circular scalpel to prepare the chondral surface and a powered trephine to prepare the osseous surface.eng
dc.description.indexMEDLINE
dc.description.indexPubMed
dc.description.indexWoS
dc.description.indexScopus
dc.description.sponsorshipJRF Ortho Investigator Initiated Grant
dc.identifier.citationCARTILAGE, v.14, n.3, p.329-337, 2023
dc.identifier.doi10.1177/19476035221141420
dc.identifier.eissn1947-6043
dc.identifier.issn1947-6035
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/54854
dc.language.isoeng
dc.publisherSAGE PUBLICATIONS INCeng
dc.relation.ispartofCartilage
dc.rightsrestrictedAccesseng
dc.rights.holderCopyright SAGE PUBLICATIONS INCeng
dc.subjectcartilageeng
dc.subjectosteochondraleng
dc.subjectchondrocyte viabilityeng
dc.subjectharvest techniqueeng
dc.subject.otherarticular-cartilageeng
dc.subject.othertransplantationeng
dc.subject.otherchondrocyteeng
dc.subject.wosOrthopedicseng
dc.titleHarvest Technique Does Affect the Quality of Osteochondral Grafts: Histologic Evaluation Comparing Commercial Standards versus Scalpel Blade Techniqueeng
dc.typearticleeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
dspace.entity.typePublication
hcfmusp.affiliation.countryEstados Unidos
hcfmusp.affiliation.countryisous
hcfmusp.author.externalMERKELY, Gergo:Harvard Med Sch, Brigham & Womens Hosp, Ctr Cartilage Repair, Dept Orthopaed Surg,Div Sports Med, Boston, MA USA; Harvard Med Sch, Brigham & Womens Hosp, Ctr Cartilage repair, Dept Orthopaed Surg,Div Sports Med, 75 Francis St, Boston, MA 02115 USA
hcfmusp.author.externalFARINA, Evan M.:Harvard Med Sch, Brigham & Womens Hosp, Ctr Cartilage Repair, Dept Orthopaed Surg,Div Sports Med, Boston, MA USA
hcfmusp.author.externalGOMOLL, Andreas H.:Hosp Special Surg, New York, NY USA
hcfmusp.author.externalLATTERMANN, Christian:Harvard Med Sch, Brigham & Womens Hosp, Ctr Cartilage Repair, Dept Orthopaed Surg,Div Sports Med, Boston, MA USA
hcfmusp.citation.scopus1
hcfmusp.contributor.author-fmusphcCHILAN BOU GHOSSON LEITE
hcfmusp.description.beginpage329
hcfmusp.description.endpage337
hcfmusp.description.issue3
hcfmusp.description.volume14
hcfmusp.origemWOS
hcfmusp.origem.pubmed36484337
hcfmusp.origem.scopus2-s2.0-85144356048
hcfmusp.origem.wosWOS:000895513500001
hcfmusp.publisher.cityTHOUSAND OAKSeng
hcfmusp.publisher.countryUSAeng
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hcfmusp.scopus.lastupdate2024-05-17
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